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391334 BES, Free Acid, ULTROL® Grade - CAS 10191-18-1 - Calbiochem

Overview

Key Spec Table

CAS #Empirical Formula
10191-18-1C₆H₁₅NO₅S

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391334-100GMCN
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      391334-25GMCN
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          Description
          OverviewBES buffer is suitable for use in systems where a calcium phosphate-DNA complex formation is desirable. Chen and Okayama described an improved transfection system for the stable transformation of cells with plasmid DNA using BES buffer. The gradual formation of the calcium phosphate-DNA complex and its slow precipitation onto cells appears to be the most critical factor contributing to the high efficiency of stable transformations.
          Absorbance (100 mM, 260 nm):≤0.05.
          Catalogue Number391334
          Brand Family Calbiochem®
          SynonymsN,N-bis-(2-Hydroxyethyl)-2-aminoethanesulfonic Acid
          References
          ReferencesDe Graeve, K.G., et al. 1994. J. Appl. Bacteriol. 76, 55.
          Chen, C.A., and Okayama, H. 1988. Biotechniques 6, 632.
          Product Information
          CAS number10191-18-1
          FormWhite solid
          Hill FormulaC₆H₁₅NO₅S
          Chemical formulaC₆H₁₅NO₅S
          PKA7.12 at 20°C
          Quality LevelMQ100
          Biological Information
          Purity≥99% by titration (dry basis)
          Physicochemical Information
          Heavy metals≤1 ppm
          Safety Information according to GHS
          RTECSKI7363500
          Storage and Shipping Information
          Ship Code Ambient Temperature Only
          Toxicity Standard Handling
          Storage +15°C to +30°C
          Do not freeze Ok to freeze
          Special InstructionsFollowing reconstitution, store in the refrigerator (4°C). Stock solutions are stable for up to 2 weeks at 4°C.
          Global Trade Item Number
          Catalogue Number GTIN
          391334-100GMCN 07790788058364
          391334-25GMCN 04055977190083