Our broad portfolio consists of multiplex panels that allow you to choose, within the panel, analytes that best meet your needs. On a separate tab you can choose the premixed cytokine format or a single plex kit.
Cell Signaling Kits & MAPmates™
Choose fixed kits that allow you to explore entire pathways or processes. Or design your own kits by choosing single plex MAPmates™, following the provided guidelines.
The following MAPmates™ should not be plexed together:
-MAPmates™ that require a different assay buffer
-Phospho-specific and total MAPmate™ pairs, e.g. total GSK3β and GSK3β (Ser 9)
-PanTyr and site-specific MAPmates™, e.g. Phospho-EGF Receptor and phospho-STAT1 (Tyr701)
-More than 1 phospho-MAPmate™ for a single target (Akt, STAT3)
-GAPDH and β-Tubulin cannot be plexed with kits or MAPmates™ containing panTyr
.
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To begin designing your MILLIPLEX® MAP kit select a species, a panel type or kit of interest.
Custom Premix Selecting "Custom Premix" option means that all of the beads you have chosen will be premixed in manufacturing before the kit is sent to you.
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Add Additional Reagents (Buffer and Detection Kit is required for use with MAPmates)
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48-602MAG
Buffer Detection Kit for Magnetic Beads
1 Kit
Space Saver Option Customers purchasing multiple kits may choose to save storage space by eliminating the kit packaging and receiving their multiplex assay components in plastic bags for more compact storage.
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505980
Sigma-AldrichAutophagy Inhibitor IV, Cpd18 - Calbiochem
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Description
Overview
A cell-permeable 3-methyladenine (3-MA) derivative that acts as an autophagy inhibitor via the inhibition of class III PI3K, and without touching class I PI3K. Shown to block 80% of starvation-induced autophagy at 1 mM (IC50 = 0.67 mM, compared with 3-MA at 6 mM). Displays much better solubility than 3-MA (5 times more soluble in water and 30 times more soluble in DMSO). Also found to reduce cell viability by 55% at 1 mM at 12 hours compared with 72% reduction for 3-MA at 10 mM.
Please note that the molecular weight for this compound is lot-specific due to variable water content.
Autophagy Inhibitor IV, Cpd18 - Calbiochem Certificates of Analysis
Title
Lot Number
505980
References
Reference overview
Wu, Y., et al, 2013. Autophagy9, 595.
Data Sheet
Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.
A cell-permeable 3-methyladenine (3-MA) derivative that acts as an autophagy inhibitor via the inhibition of class III PI3K, and without touching class I PI3K. Shown to block 80% of starvation - induced autophagy at 1 mM (IC50 = 0.67 mM, compared with 3-MA at 6 mM). Displays much better solubility than 3-MA (5 times more soluble in water and 30 times more soluble in DMSO). Also found to reduce cell viability by 55% at 1 mM at 12 hours compared with 72% reduction for 3-MA at 10 mM.
Form
White solid
Chemical formula
C₁₂H₁₇N₅
Structure formula
Purity
≥98% by HPLC
Solubility
DMSO (50 mg/ml)
Storage
Protect from light +2°C to +8°C
Do Not Freeze
Ok to freeze
Special Instructions
Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.
