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ABS219 Anti-phospho-Tie2 (Ser1119) Antibody

Anti-phospho-Tie2 (Ser1119) Antibody is an antibody against phospho-Tie2 (Ser1119) for use in WB.

Anti-phospho-Tie2 (Ser1119) Antibody MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Signaling Research Sub-Category: Kinases & Phosphatases Gene Symbol: TEK, TIE2 UniProt Number: Q02763
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ABS219
100 µL  
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      Overview

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      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, B, M, RWBRbAffinity PurifiedPolyclonal Antibody
      Description
      Catalogue NumberABS219
      DescriptionAnti-phospho-Tie2 (Ser1119) Antibody
      Alternate Names
      • Angiopoietin-1 receptor
      • Tunica interna endothelial cell kinase
      • Tyrosine-protein kinase receptor TEK
      • Tyrosine-protein kinase receptor TIE-2
      • hTIE2
      • p140 TEK
      • CD202b
      Background InformationTIE2 (tyrosine kinase with Ig and EGF homology domains 2) is expressed almost exclusively in endothelial cells in mice, rats and humans. This receptor possesses a unique extracellular domain containing two immunoglobulin-like loops separated by three epidermal growth factor-like repeats that are connected to three fibronectin type III-like repeats. The ligand for the receptor is Angiopoietin 1. Defects in TIE2 are associated with inherited venous malformations; the TIE2 signaling pathway appears to be critical for endothelial cell-smooth muscle cell communication in venous morphogenesis.
      References
      Product Information
      FormatAffinity Purified
      Control
      • HEK293T overexpressing Tie2 protein
      PresentationPurified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationAnti-phospho-Tie2 (Ser1119) Antibody is an antibody against phospho-Tie2 (Ser1119) for use in WB.
      Key Applications
      • Western Blotting
      Biological Information
      ImmunogenKLH-conjugated linear peptide corresponding to human Tie2 phosphorylated at Ser1119.
      EpitopePhosphorylated Ser1119
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostRabbit
      SpecificityThis antibody recognizes Tie2 phosphorylated at Ser1119.
      Species Reactivity
      • Human
      • Bovine
      • Mouse
      • Rat
      Species Reactivity NoteDemonstrated to react with Human. Predicted to react with Bovine, Mouse, and Rat based on 100% sequence homology.
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThe TEK receptor tyrosine kinase is expressed almost exclusively in endothelial cells in mice, rats, and humans. This receptor possesses a unique extracellular domain containing 2 immunoglobulin-like loops separated by 3 epidermal growth factor-like repeats that are connected to 3 fibronectin type III-like repeats. The ligand for the receptor is angiopoietin-1. Defects in TEK are associated with inherited venous malformations; the TEK signaling pathway appears to be critical for endothelial cell-smooth muscle cell communication in venous morphogenesis. TEK is closely related to the TIE receptor tyrosine kinase. [provided by RefSeq, Jul 2008].
      Gene Symbol
      • TEK
      • TIE2
      Modifications
      • Phosphorylation
      Purification MethodAffinity Purfied
      UniProt Number
      UniProt SummaryFUNCTION: This protein is a protein tyrosine-kinase transmembrane receptor for angiopoietin 1. It may constitute the earliest mammalian endothelial cell lineage marker. Probably regulates endothelial cell proliferation, differentiation and guides the proper patterning of endothelial cells during blood vessel formation.

      CATALYTIC ACTIVITY: ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate.

      SUBUNIT STRUCTURE: Interacts with PTPRB.

      SUBCELLULAR LOCATION: Membrane; Single-pass type I membrane protein.

      TISSUE SPECIFICITY: Predominantly expressed in endothelial cells and their progenitors, the angioblasts. Has been directly found in placenta and lung, with a lower level in umbilical vein endothelial cells, brain and kidney.

      PTM: Phosphorylated. Phosphorylation is induced by ANGPT1 and ANGPT2. ANGPT1-induced phosphorylation is impaired during hypoxia. Dephosphorylated by PTPRB.

      Autophosphorylation at Tyr-1108 is critical for coupling downstream cell migration signal transduction pathways with ANG1 stimulation in endothelial cells (By similarity).

      INVOLVEMENT IN DISEASE: Defects in TEK are a cause of dominantly inherited venous malformations (VMCM) [MIM:600195]; an error of vascular morphogenesis characterized by dilated, serpiginous channels.

      SEQUENCE SIMILARITIES: Belongs to the protein kinase superfamily. Tyr protein kinase family. Tie subfamily.

      Contains 3 EGF-like domains.

      Contains 3 fibronectin type-III domains.

      Contains 2 Ig-like C2-type (immunoglobulin-like) domains.

      Contains 1 protein kinase domain.
      Molecular Weight~160 kDa observed. Uniprot gives a calculated molecular weight of 126 kDa, but has been observed at ~160 kDa due to glycosolation. A non-modified specific band appears at ~52 kDa in some lysates.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blot in HEK293T overexpressing Tie2 protein.

      Western Blot Analysis: A 1:500 dilution detected Tie2 on 10 µg of HEK293T overexpressing Tie2 protein.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      ABS219 04053252592461

      Documentation

      Anti-phospho-Tie2 (Ser1119) Antibody SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-phospho-Tie2 (Ser1119) Antibody Certificates of Analysis

      TitleLot Number
      Anti-phospho-Tie2 (Ser1119) - 2266772 2266772
      Anti-phospho-Tie2 (Ser1119) - 2281675 2281675
      Anti-phospho-Tie2 (Ser1119) - 3198461 3198461
      Anti-phospho-Tie2 (Ser1119) - 3205951 3205951
      Anti-phospho-Tie2 (Ser1119) - 3237542 3237542
      Anti-phospho-Tie2 (Ser1119) - 3753314 3753314
      Anti-phospho-Tie2 (Ser1119) - NRG1929418 NRG1929418
      Anti-phospho-Tie2 (Ser1119) -2720124 2720124
      Anti-phospho-Tie2 (Ser1119) -2803237 2803237
      Anti-phospho-Tie2 (Ser1119) Polyclonal Antibody 3105316
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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies