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ABE332 Anti-phospho-IGF-1R (Tyr1161/Tyr1165/Tyr1166) Antibody

Anti-phospho-IGF-1R (Tyr1161/Tyr1165/Tyr1166) Antibody is an antibody against phospho-IGF-1R (Tyr1161/Tyr1165/Tyr1166) for use in WB, Cell Function Assay.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Epigenetics & Nuclear Function, Signaling Research Sub-Category: Insulin/Energy Signaling, Insulin/Energy Signaling Gene Symbol: IGF1R UniProt Number: P08069
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ABE332
200 µL  
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      Overview

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      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, R, M, Po, Xn, ChWB, Cell Function AssayRbAffinity PurifiedPolyclonal Antibody
      Description
      Catalogue NumberABE332
      DescriptionAnti-phospho-IGF-1R (Tyr1161/Tyr1165/Tyr1166) Antibody
      Alternate Names
      • Insulin-like growth factor 1 receptor
      • Insulin-like growth factor I receptor
      • IGF-I receptor
      • CD221
      • Insulin-like growth factor 1 receptor alpha chain
      • Insulin-like growth factor 1 receptor beta chain
      Background InformationThere are two kinds of IGF receptors based on their relative affinities for IGF-1 versus IGF-2. The IGF-1 receptor (IGF-1R) prefers IGF-1 over IGF-2 and weakly binds insulin. IGF-1 receptor is a disulfide-linked heterotetrameric transmembrane protein consisting of two alpha (130 kDa) and two beta (95 kDa) subunits. Both the alpha and beta subunits are encoded within a single receptor precursor cDNA. The IGF-1 receptor is therefore similar in structure to the insulin receptor. The proreceptor polypeptide is proteolytically cleaved and disulfide-linked to yield the mature heterotetrameric receptor. The IGF-1 receptor is highly expressed in all cell types and tissues and is highly overexpressed in most malignant tissues where it functions as an anti-apoptotic agent by mediating signaling pathways that enhance cell survival.
      References
      Product Information
      FormatAffinity Purified
      Control
      • IGF1 untreated and treated HEK293 cell lysate
      PresentationPurified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationAnti-phospho-IGF-1R (Tyr1161/Tyr1165/Tyr1166) Antibody is an antibody against phospho-IGF-1R (Tyr1161/Tyr1165/Tyr1166) for use in WB, Cell Function Assay.
      Key Applications
      • Western Blotting
      • Cell Function Assay
      Application NotesWestern Blot Peptide Inhibition Analysis: A representative lot detected IGF-1R in IGF1 treated HEK293 cell lysate.
      Biological Information
      ImmunogenKLH-conjugated linear peptide corresponding to human IGF-1R phosphorylated at Tyr1161/Tyr1165/Tyr1166.
      EpitopePhosphorylated Tyr1161/Tyr1165/Tyr1166.
      HostRabbit
      SpecificityThis antibody recognizes IGF-1R phosphorylated at Tyr1161/Tyr1165/Tyr1166. Please note that some sources list the phsophorylation sites as Tyr1131/Tyr1135/Tyr1136. The difference in the amino acid sequence is due to a 30 amino acid signal chain that is removed due to protein processing.
      Species Reactivity
      • Human
      • Rat
      • Mouse
      • Pig
      • Xenopus
      • Chicken
      Species Reactivity NoteDemonstrated to react with Human. Predicted to react with Rat, Mouse, Porcine, Xenopus, Chicken, and Zebra Fish based on 100% sequence homology.
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThis receptor binds insulin-like growth factor with a high affinity. It has tyrosine kinase activity. The insulin-like growth factor I receptor plays a critical role in transformation events. Cleavage of the precursor generates alpha and beta subunits. It is highly overexpressed in most malignant tissues where it functions as an anti-apoptotic agent by enhancing cell survival.
      Gene Symbol
      • IGF1R
      Modifications
      • Phosphorylation
      • Phosphorylation
      • Phosphorylation
      Purification MethodAffinity Purfied
      UniProt Number
      UniProt SummaryFUNCTION: This receptor binds insulin-like growth factor 1 (IGF1) with a high affinity and IGF2 with a lower affinity. It has a tyrosine-protein kinase activity, which is necessary for the activation of the IGF1-stimulated downstream signaling cascade. When present in a hybrid receptor with INSR, binds IGF1. Hybrid receptors composed of IGF1R and INSR isoform Long are activated with high affinity by IGF1 and with low affinity by IGF2, but are not significantly activated by insulin. On the other hand, hybrid receptors composed of IGF1R and INSR isoform Short are activated by IGF1, IGF2 and insulin. Additionally, hybrid receptors composed of IGF1R and INSR isoform Long and hybrid receptors composed of IGF1R and INSR isoform Short have similar binding characteristics, where both receptor types bind IGF1 and have a low affinity for insulin.

      CATALYTIC ACTIVITY: ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate.

      COFACTOR: Manganese.

      ENZYME REGULATION: Autophosphorylation activates the kinase activity.

      SUBUNIT STRUCTURE: Tetramer of 2 alpha and 2 beta chains linked by disulfide bonds. The alpha chains contribute to the formation of the ligand-binding domain, while the beta chain carries the kinase domain. Interacts with PIK3R1 and with the PTB/PID domains of IRS1 and SHC1 in vitro when autophosphorylated on tyrosine residues. Forms a hybrid receptor with INSR, the hybrid is a tetramer consisting of 1 alpha chain and 1 beta chain of INSR and 1 alpha chain and 1 beta chain of IGF1R. Interacts with ARRB1 and ARRB2. Interacts with GRB10. Interacts with GNB2L1/RACK1.

      SUBCELLULAR LOCATION: Membrane; Single-pass type I membrane protein.

      TISSUE SPECIFICITY: Found as a hybrid receptor with INSR in muscle, heart, kidney, adipose tissue, skeletal muscle, hepatoma, fibroblasts, spleen and placenta (at protein level). Expressed in a variety of tissues.
      Molecular Weight~95 kDa observed. IGF-1R can be cleaved into an Insulin-like growth factor 1 receptor alpha (aa 31-736) and beta (aa 741-1367) chain. Both chains are known to contain multiple potential glycosylation sites. Distinct beta-subunits are present in hybrid insulin-like-growth-factor-1 receptors in the central nervous system. (Moss, A.M., et al. (1993). Biochem J. 294( Pt 3):685-692.)
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blot in IGF1treated HEK293 cell lysate.

      Western Blot Analysis: A 1:200 dilution of this antibody detected IGF-1R in IGF1 treated HEK293 cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size200 µL
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      ABE332 04053252686139

      Documentation

      Anti-phospho-IGF-1R (Tyr1161/Tyr1165/Tyr1166) Antibody SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-phospho-IGF-1R (Tyr1161/Tyr1165/Tyr1166) Antibody Certificates of Analysis

      TitleLot Number
      Anti-phospho-IGF-1R (Tyr1161/Tyr1165/Tyr1166) 2471001
      Anti-phospho-IGF-1R (Tyr1161/Tyr1165/Tyr1166) - 2147088 2147088
      Anti-phospho-IGF-1R (Tyr1161/Tyr1165/Tyr1166) - 2379501 2379501
      Anti-phospho-IGF-1R (Tyr1161/Tyr1165/Tyr1166) - 2452160 2452160
      Anti-phospho-IGF-1R (Tyr1161/Tyr1165/Tyr1166) - 2007350 2007350
      Anti-phospho-IGF-1R (Tyr1161/Tyr1165/Tyr1166) - 2205945 2205945
      Anti-phospho-IGF-1R (Tyr1161/Tyr1165/Tyr1166) - 2341105 2341105
      Anti-phospho-IGF-1R (Tyr1161/Tyr1165/Tyr1166) - 3169882 3169882
      Anti-phospho-IGF-1R (Tyr1161/Tyr1165/Tyr1166) - 3259075 3259075
      Anti-phospho-IGF-1R (Tyr1161/Tyr1165/Tyr1166) - 3407326 3407326
      Show more

      Brochure

      Title
      New Products: Volume 3, 2012
      Product Selection Guide - Antibodies, small molecule inhibitors, kits, assays and proteins for signaling research.

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies