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MABN833 Anti-phospho Drebrin (Ser142), clone 3C14 Antibody

Anti-phospho Drebrin (Ser142), clone 3C14 | MABN833 is an antibody against phospho Drebrin for use in Western Blotting, Immunocytochemistry, Peptide Inhibition Assay.

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Research Category: Neuroscience Research Sub-Category: Developmental Signaling Gene Symbol: Dbn1 UniProt Number: Q07266
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MABN833
100 µg  
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      Overview

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      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      R, H, MWB, ICC, PIAMPurifiedMonoclonal Antibody
      Description
      Catalogue NumberMABN833
      DescriptionAnti-phospho Drebrin (Ser142), clone 3C14 Antibody
      Alternate Names
      • Drebrin
      • Developmentally-regulated brain protein
      Background InformationDevelopmentally-regulated brain protein, also known as Drebrin, and encoded by the gene name DBN1, is a developmentally-regulated neuron-specific protein, expressed during outgrowth and maturation of dendrites, and localized within dendrites of the adult brain. Drebrin is required for actin polymerization at immunological synapses (IS) and for CXCR4 recruitment to IS. Recent studies have shown that Drebrins may play a role in cell migration, extension of neuronal processes and plasticity of dendrites. Drebrins are classified into two forms of the embryonic type (E1 and E2) and one form of the adult type (A). The time course of their appearance are different from each other. Their structures are closely related. Adult rat brain expresses only drebrin A while drebrin E1 or E2 is observed in immature animals. Drebrin E+A- signals have been observed in the cell bodies of migrating neuroblasts in the core region; however, only fibrous and punctate drebrin E+A- signals have been observed in postmigratory neuroblasts at the outer layers. Studies have demonstrated that the disappearance of Drebrin E+A- signals from the cell body coincides with the cessation of neuronal migration. The disappearance of Drebrin E from the cell body may be a molecular switch for the cessation of migration in newly generated neuroblast. Drebrin is expressed mainly in brain neurons.
      Product Information
      FormatPurified
      PresentationPurified mouse monoclonal IgG3κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationAnti-phospho Drebrin (Ser142), clone 3C14 | MABN833 is an antibody against phospho Drebrin for use in Western Blotting, Immunocytochemistry, Peptide Inhibition Assay.
      Key Applications
      • Western Blotting
      • Immunocytochemistry
      • Peptide Inhibition Assay
      Application NotesWestern Blotting Analysis: 0.5 µg/mL from a representative lot detected phospho Drebrin (Ser142) in 10 µg of rat neonatal brain tissue lysate.
      Immunocytochemistry Analysis: 20 µg/mL from a representative lot detected phospho Drebrin (Ser142) in HeLa and NIH/3T3 cells.
      Peptide Inhibition Analysis: 0.5 µg/mL from a representative lot detected phospho Drebrin (Ser142) in 10 µg of rat neonatal brain tissue lysate.
      Immunocytochemistry Analysis: A representative lot detected phospho Drebrin (Ser142) in rat embryonic cortical culture (Prof. Phillip R. Gordon-Weeks, King's College London).
      Biological Information
      ImmunogenKLH-conjugated linear peptide corresponding to rat phospho Drebrin (Ser142).
      Clone3C14
      ConcentrationPlease refer to lot specific datasheet.
      HostMouse
      IsotypeIgG3κ
      Species Reactivity
      • Rat
      • Human
      • Mouse
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • Dbn1
      Purification MethodProtein G Purified
      UniProt Number
      Molecular Weight~100 kDa observed. The calculated molecular weight is 71 kDa, however phospho Drebrin (Ser142) has been shown as a ~100 kDa band in western blots (Worth, D.C., et al. (2013). JCB. 202(5):793-806).
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in SH-SY5Y cell lysate.

      Western Blotting Analysis: 0.5 µg/mL of this antibody detected phospho Drebrin (Ser142) in 10 µg of SH-SY5Y cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µg
      Global Trade Item Number
      Catalogue Number GTIN
      MABN833 04055977123876