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OP39 Anti-c-ErbB2/c-Neu (Ab-5) Mouse mAb (TA-1)

Anti-c-ErbB2/c-Neu (Ab-5), mouse monoclonal, clone TA-, recognizes the ~185 kDa c-ErbB2/c-Neu protein in SK-BR-3 cells. It is validated for FC, WB, IF, IP, and IHC on frozen sections.

Anti-c-ErbB2/c-Neu (Ab-5) Mouse mAb (TA-1) MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: Anti-HER2, Anti-c-Neu, Anti-ErbB2, Anti-Erythroblastosis Virus

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OP39
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Overview

Replacement Information

Key Spec Table

Species ReactivityHostAntibody Type
HMMonoclonal Antibody

Pricing & Availability

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OP39-100UGCN
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      		 Plastic ampoule 100 μg
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      Description
      OverviewRecognizes the ~185 kDa c-ErbB2/c-Neu protein in SK-BR-3 cells.
      Catalogue NumberOP39
      Brand Family Calbiochem®
      SynonymsAnti-HER2, Anti-c-Neu, Anti-ErbB2, Anti-Erythroblastosis Virus
      References
      ReferencesDiFiore, P.P., et al. 1987. Science 237, 178.
      Slamon, D.J., et al. 1987. Science 235, 177.
      Varley, J.M., et al. 1987. Oncogene 1, 423.
      Yamamoto, T., et al. 1986. Nature 319, 230.
      Blick, M., et al. 1984. Blood 64, 1234.
      Schwab, M., et al. 1984 Cold Spring Harbor Laboratory 2, 215.
      Bargmann, C.I., et al. 1968. Nature 319, 226.
      Product Information
      FormLiquid
      FormulationIn 50 mM PBS, 0.2% gelatin, pH 7.5.
      Negative controlHBL 100 cells
      Positive controlSK-BR-3 cells
      Preservative≤0.1% sodium azide
      Quality LevelMQ100
      Applications
      Application ReferencesOriginal Clone, Immunoprecipitation, Immunofluorescence, Flow Cytometry Montgomery, R.B., et al. 2005. Cancer Res. 65, 650. McKenzie, S.J., et al. 1989. Oncogene 4, 543. Immunoblotting Montgomery, R.B., et al. 2005. Cancer Res. 65, 650. Not for Neutralization Studies McKenzie, S.J., et al. 1989. Oncogene 4, 543.
      Key Applications Flow Cytometry
      Frozen Sections
      Immunoblotting (Western Blotting)
      Immunofluorescence
      Immunoprecipitation
      Not Neutralization Studies
      Application NotesFlow Cytometry (5 µg/ml, see application references)
      Frozen Sections (5 µg/ml)
      Immunoblotting (see application references)
      Immunofluorescence (5 µg/ml, see application references)
      Immunoprecipitation (1 µg/sample, see application references)
      Neutralization Studies (not recommended, see comments and application references)
      Application CommentsRecognizes an extracellular epitope and does not cross react with human EGF-receptor. It will not inhibit in vivo protein tyrosine kinase activity. Antibody should be titrated for optimal results in individual systems.
      Biological Information
      Immunogencells overexpressing human c-ErbB2/c-Neu
      ImmunogenHuman
      EpitopeWithin the extracellular domain
      CloneTA-1
      HostMouse
      IsotypeIgG₁
      Species Reactivity
      • Human
      Antibody TypeMonoclonal Antibody
      Concentration Label Please refer to vial label for lot-specific concentration
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Blue Ice Only
      Toxicity Standard Handling
      Storage +2°C to +8°C
      Do not freeze Yes
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      OP39-100UGCN 04055977227383

      Documentation

      Anti-c-ErbB2/c-Neu (Ab-5) Mouse mAb (TA-1) SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-c-ErbB2/c-Neu (Ab-5) Mouse mAb (TA-1) Certificates of Analysis

      TitleLot Number
      OP39

      References

      Reference overview
      DiFiore, P.P., et al. 1987. Science 237, 178.
      Slamon, D.J., et al. 1987. Science 235, 177.
      Varley, J.M., et al. 1987. Oncogene 1, 423.
      Yamamoto, T., et al. 1986. Nature 319, 230.
      Blick, M., et al. 1984. Blood 64, 1234.
      Schwab, M., et al. 1984 Cold Spring Harbor Laboratory 2, 215.
      Bargmann, C.I., et al. 1968. Nature 319, 226.

      Citations

      Title
    • R. Bruce Montgomery, et al. (2005) Endogenous Anti-HER2 Antibodies Block HER2 Phosphorylation and Signaling through Extracellular Signal-Regulated Kinase. Cancer Research 65, 650-656.
      		•
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision02-October-2007 RFH
      SynonymsAnti-HER2, Anti-c-Neu, Anti-ErbB2, Anti-Erythroblastosis Virus
      ApplicationFlow Cytometry (5 µg/ml, see application references)
      Frozen Sections (5 µg/ml)
      Immunoblotting (see application references)
      Immunofluorescence (5 µg/ml, see application references)
      Immunoprecipitation (1 µg/sample, see application references)
      Neutralization Studies (not recommended, see comments and application references)
      DescriptionPurified mouse monoclonal antibody generated by immunizing BALB/c mice with the specified immunogen and fusing spleonocytes with SP2/0 mouse myeloma cells (see application references). Recognizes the c-ErbB2/c-neu protein.
      BackgroundAmplification of the human proto-oncogene c-neu occurs in approximately 30% of breast carcinomas and the amplification maybe a useful predictor of disease prognosis. Gene amplification and the resulting overexpression of proto-oncogene encoded proteins is thought to transform cells by chronically stimulating signal transduction pathways and in model systems overexpression of human c-neu induces cellular transformation. In the rat model system, a point mutation has been located within the transmembrane domain of the c-neu oncogene.
      HostMouse
      Immunogen speciesHuman
      Immunogencells overexpressing human c-ErbB2/c-Neu
      EpitopeWithin the extracellular domain
      CloneTA-1
      IsotypeIgG₁
      Specieshuman, not mouse, not rat
      Positive controlSK-BR-3 cells
      Negative controlHBL 100 cells
      FormLiquid
      FormulationIn 50 mM PBS, 0.2% gelatin, pH 7.5.
      Concentration Label Please refer to vial label for lot-specific concentration
      Preservative≤0.1% sodium azide
      CommentsRecognizes an extracellular epitope and does not cross react with human EGF-receptor. It will not inhibit in vivo protein tyrosine kinase activity. Antibody should be titrated for optimal results in individual systems.
      Storage +2°C to +8°C
      Do Not Freeze Yes
      Toxicity Standard Handling
      ReferencesDiFiore, P.P., et al. 1987. Science 237, 178.
      Slamon, D.J., et al. 1987. Science 235, 177.
      Varley, J.M., et al. 1987. Oncogene 1, 423.
      Yamamoto, T., et al. 1986. Nature 319, 230.
      Blick, M., et al. 1984. Blood 64, 1234.
      Schwab, M., et al. 1984 Cold Spring Harbor Laboratory 2, 215.
      Bargmann, C.I., et al. 1968. Nature 319, 226.
      Citation
    • R. Bruce Montgomery, et al. (2005) Endogenous Anti-HER2 Antibodies Block HER2 Phosphorylation and Signaling through Extracellular Signal-Regulated Kinase. Cancer Research 65, 650-656.
      		•
      Application referencesOriginal Clone, Immunoprecipitation, Immunofluorescence, Flow Cytometry Montgomery, R.B., et al. 2005. Cancer Res. 65, 650. McKenzie, S.J., et al. 1989. Oncogene 4, 543. Immunoblotting Montgomery, R.B., et al. 2005. Cancer Res. 65, 650. Not for Neutralization Studies McKenzie, S.J., et al. 1989. Oncogene 4, 543.