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04-263 Anti-Ubiquitinylated proteins Antibody, clone FK2

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04-263
100 µL  
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      Overview

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      AELISA, IF, IP, WBMPurifiedMonoclonal Antibody
      Description
      Catalogue Number04-263
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionAnti-Ubiquitinylated proteins Antibody, clone FK2
      Background InformationUbiquitin exists either covalently attached to another protein, or free (unanchored). When covalently bound, it is conjugated to target proteins via an isopeptide bond either as a monomer (monoubiquitin), a polymer linked via different Lys residues of the ubiquitin (polyubiquitin chains) or a linear polymer linked via the initiator Met of the ubiquitin (linear polyubiquitin chains). Polyubiquitin chains, when attached to a target protein, have different functions depending on the Lys residue of the ubiquitin that is linked: Lys-6-linked may be involved in DNA repair; Lys-11-linked is involved in ERAD (endoplasmic reticulum-associated degradation) and in cell-cycle regulation; Lys-29-linked is involved in lysosomal degradation; Lys-33-linked is involved in kinase modification; Lys-48-linked is involved in protein degradation via the proteasome; Lys-63-linked is involved in endocytosis, DNA-damage responses as well as in signaling processes leading to activation of the transcription factor NF-kappa-B. Linear polymer chains formed via attachment by the initiator Met lead to cell signaling. Ubiquitin is usually conjugated to Lys residues of target proteins, however, in rare cases, conjugation to Cys or Ser residues has been observed. When polyubiquitin is free (unanchored-polyubiquitin), it also has distinct roles, such as in activation of protein kinases, and in signaling. Similarity: Belongs to the ubiquitin family. Contains 3 ubiquitin-like domains.
      Product Information
      FormatPurified
      Presentation100 µL of purified immunoglobulin in PBS, pH 7.4 containing 50% glycerol and 0.09% sodium azide. Dilute to working strength with phosphate buffered saline pH 7.2-7.4.
      Quality LevelMQ100
      Applications
      ApplicationThis Anti-Ubiquitinylated proteins Antibody, clone FK2 has been published and validated for use in ELISA, Immunofluorescence (IF), Immunoprecipitation (IP) and Western Blotting (WB) for the detection of Ubiquitinylated proteins.
      Key Applications
      • ELISA
      • Immunofluorescence
      • Immunoprecipitation
      • Western Blotting
      Biological Information
      ImmunogenPoly-ubiquitinylated-lysozyme.
      Cloneclone FK2
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostMouse
      SpecificityRecognizes mono- and poly ubiquitinylated proteins, but not free ubiquitin.
      IsotypeIgG1
      Species Reactivity
      • All
      Antibody TypeMonoclonal Antibody
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20°C from date of shipment for up to 12 months.
      Packaging Information
      Material Size100 µL
      Global Trade Item Number
      Catalogue Number GTIN
      04-263 04053252627774