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AB9332-200UL Anti-Transient Receptor Potential Vanilloid 4 Antibody, pain

Detect Transient Receptor Potential Vanilloid 4 using this Anti-Transient Receptor Potential Vanilloid 4 Antibody, pain validated for use in WB.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Neuroscience Research Sub-Category: Ion Channels & Transporters Gene Symbol: TRPV4, VROAC, TRP12, VRL2, VR-OAC, OTRPC4, VRL-2, TrpV4 UniProt Number: Q9ERZ8
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AB9332-200UL
200 µL  
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      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      RWBRbAffinity PurifiedPolyclonal Antibody
      Description
      Catalogue NumberAB9332-200UL
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionAnti-Transient Receptor Potential Vanilloid 4 Antibody, pain
      Alternate Names
      • TRPV4
      References
      Product Information
      FormatAffinity Purified
      Control
      • Included free of charge with the antibody is 40 μg of control antigen (lyophilized powder). The stock solution of the antigen can be made up using 100 μL of sterile deionized water. For negative control, preincubate 1 μg of peptide with 1 μg of antibody for one hour at room temperature. Optimal concentrations must be determined by the end user.
      PresentationAffinity purified immunoglobulin. Lyophilized from phosphate buffered saline, pH 7.4, containing 1% BSA, and 0.05% sodium azide as a preservative. Reconstitute with 200 μL of sterile deionized water. Centrifuge antibody preparation before use (10,000 xg for 5 min).
      Quality LevelMQ100
      Applications
      ApplicationDetect Transient Receptor Potential Vanilloid 4 using this Anti-Transient Receptor Potential Vanilloid 4 Antibody, pain validated for use in WB.
      Key Applications
      • Western Blotting
      Application NotesWestern blot: 1:200 using ECL on ND7/23 lysate.

      Immunohistochemistry on rat brain and mouse cerebellum frozen sections.

      Dilutions should be made using a carrier protein such as BSA (1-3%)

      Optimal working dilutions must be determined by the end user.

      SUGGESTED WESTERN BLOT PROTOCOL



      1. Mix the samples (organ membranes: 50 μg/lane; transfected cells: 500,000 cells/lane) with sample-buffer X 2, and heat 10 min at 70°C.

      2. 5-50 μL applied to Minigel lane (0.75-1.5 mm width) and run at standard conditions. (60 mA for 2 1.5 mm Minigel gels, 1.4 h). It is suggested that you run 5-15% acrylamide (37.5:1 acrylamide:bisacrysmide) minigel (1.5 mm width) at 30 mA/gel ~1-1.5 hours.

      3. Transfer in semi-dry system under standard conditions (3 h 100 mA for two minigel gels)

      4. Stain the transferred bands with Chemicon BLOT-FastStain (Catalog Number 2076).

      5. Destain with deionized water.

      6. Block with 5% non-fat milk (Marvel or Carnation) in PBS, and 0.025 % sodium azide, overnight at 2-8°C. The non-fat milk should be dissolved freshly, centrifuged 10,000 rpm for

      10 min, and filtered through glass filter (Gelman Acrodisc).

      7. Incubation with first antibody 2 h at room temperature or overnight at 4°C in blocking solution. The antibody preparation should be centrifuged before use (10,000 g 5 min.). Optimal working dilutions and incubation time will need to be determined by the end user.

      8. Wash 4 x 10 min. with PBS-0.1% tween 20. From this stage, azide should be omitted.

      9. Incubation with the secondary antibody (HRP-conjugated goat anti-rabbit antibody, for example Chemicon Catalog Number AP132P, diluted appropriately) 1 h at room temperature.

      10. Wash 4 x 10 min. with PBS-0.1% tween 20.

      11. Perform ECL with commercial kits (Chemilucent, Chemicon Catalog Number 2600).
      Biological Information
      ImmunogenPurified peptide from amino acids 853-871 of rat TRVP4 (Accession number Q9ERZ8).
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostRabbit
      SpecificityRecognizes TRPV4 (Transient Receptor Potential Vanilloid 4).
      Species Reactivity
      • Rat
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThis gene encodes a member of the OSM9-like transient receptor potential channel (OTRPC) subfamily in the transient receptor potential (TRP) superfamily of ion channels. The encoded protein is a Ca2+-permeable, nonselective cation channel that is thought to be involved in the regulation of systemic osmotic pressure. Two transcript variants encoding different isoforms have been found for this gene.
      Gene Symbol
      • TRPV4
      • VROAC
      • TRP12
      • VRL2
      • VR-OAC
      • OTRPC4
      • VRL-2
      • TrpV4
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: Q9HBA0 # Non-selective calcium permeant cation channel probably involved in osmotic sensitivity and mechanosensitivity. Activation by exposure to hypotonicity within the physiological range exhibits an outward rectification. Also activated by low pH, citrate and phorbol esters. Increase of intracellular Ca(2+) potentiates currents. Channel activity seems to be regulated by a calmodulin-dependent mechanism with a negative feedback mechanism.
      SIZE: 871 amino acids; 98281 Da
      SUBUNIT: Homotetramer (Probable). Self-associates in a isoform- specific manner. Isoforms 1/A and 5/D but not isoform 2/B, 4/C and 6/E can oligomerize. Interacts with calmodulin. Interacts with Map7 and Src family Tyr protein kinases LYN, SRC, FYN, HCK, LCK and YES (By similarity).
      SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein (Probable). Note=Assembly of the putative homotetramer occurs primarily in the endoplasmic reticulum. & Isoform 1: Cell membrane. & Isoform 5: Cell membrane.
      SIMILARITY: SwissProt: Q9HBA0 ## Belongs to the transient receptor family. TrpV subfamily. & Contains 3 ANK repeats.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsMaintain lyophilized material at -20°C for up to 12 months after date of receipt. After reconstitution maintain at -20°C in undiluted aliquots for up to 6 months. Avoid repeated freeze/thaw cycles.
      Packaging Information
      Material Size200 µL
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      AB9332-200UL 04053252366635

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies