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AM76 Anti-TBK1 Mouse mAb (108A429)

This Anti-TBK1 Mouse mAb (108A429) is validated for use in Immunoblotting for the detection of TBK1.

Anti-TBK1 Mouse mAb (108A429) MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
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AM76
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Overview

Replacement Information

Key Spec Table

Species ReactivityHostAntibody Type
HMMonoclonal Antibody

Pricing & Availability

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AM76-100UGCN
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      		 Plastic ampoule 100 μg
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      Description
      OverviewRecognizes the ~80 kDa TBK1 protein in HEK293 cells.
      Catalogue NumberAM76
      Brand Family Calbiochem®
      Application Data
      Detection of human TBK1 by immunoblotting. Samples: Whole cell lysates from 293 cells untransfected (lane 1) and transfected with human TBK1 cDNA (lane 2). Primary antibody: Anti-TBK1 Mouse mAb (108A429) (Cat. No. AM76) (2 µg/ml). Detection: chemiluminescence.

      Detection of TBK1 by immunoblotting. Samples: Whole cell lysates from human Daudi cells (lane 1) and mouse RAW cells (lane 2). Primary antibody: Anti-TBK1 Mouse mAb (108A429) (Cat. No. AM76) (2 µg/ml). Detection: chemiluminescence.
      References
      ReferencesPomeerantz, J.L. and Baltimore, D. 1999. EMBO J. 18, 6694.
      Didonato, J.A., et al. 1997. Nature 388, 548.
      Lee, F.S., et al. 1997. Cell 88, 213.
      Malinin, N.L., et al. 1997. Nature 385, 540.
      Mercurio, F., et al. 1997. Science 278, 860.
      Regnier, C.H., et al. 1997. Cell 90, 373.
      Verma, I. and Stevenson, J.K. 1997. Proc. Natl. Acad. Sci. USA 94, 11758.
      Verma, I.M., et al. 1995. Genes Dev. 9, 2723.
      Product Information
      FormLiquid
      FormulationIn PBS, 0.2% gelatin.
      Negative controlNormal mouse serum
      Positive controlHEK293 cells
      Preservative≤0.1% sodium azide
      Quality LevelMQ100
      Applications
      Key Applications Immunoblotting (Western Blotting)
      Application NotesImmunoblotting (2 µg/ml)
      Application CommentsAntibody should be titrated for optimal results in an individual systems.
      Biological Information
      Immunogenfull-length, recombinant, human TBK1 fused to a His•Tag® sequence
      ImmunogenHuman
      Clone108A429
      HostMouse
      IsotypeIgG₁
      Species Reactivity
      • Human
      Antibody TypeMonoclonal Antibody
      Concentration Label Please refer to vial label for lot-specific concentration
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Blue Ice Only
      Toxicity Standard Handling
      Storage -20°C
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      AM76-100UGCN 04055977228045

      Documentation

      Anti-TBK1 Mouse mAb (108A429) SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-TBK1 Mouse mAb (108A429) Certificates of Analysis

      TitleLot Number
      AM76

      References

      Reference overview
      Pomeerantz, J.L. and Baltimore, D. 1999. EMBO J. 18, 6694.
      Didonato, J.A., et al. 1997. Nature 388, 548.
      Lee, F.S., et al. 1997. Cell 88, 213.
      Malinin, N.L., et al. 1997. Nature 385, 540.
      Mercurio, F., et al. 1997. Science 278, 860.
      Regnier, C.H., et al. 1997. Cell 90, 373.
      Verma, I. and Stevenson, J.K. 1997. Proc. Natl. Acad. Sci. USA 94, 11758.
      Verma, I.M., et al. 1995. Genes Dev. 9, 2723.
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision17-August-2007 RFH
      ApplicationImmunoblotting (2 µg/ml)
      Application Data
      Detection of human TBK1 by immunoblotting. Samples: Whole cell lysates from 293 cells untransfected (lane 1) and transfected with human TBK1 cDNA (lane 2). Primary antibody: Anti-TBK1 Mouse mAb (108A429) (Cat. No. AM76) (2 µg/ml). Detection: chemiluminescence.

      Detection of TBK1 by immunoblotting. Samples: Whole cell lysates from human Daudi cells (lane 1) and mouse RAW cells (lane 2). Primary antibody: Anti-TBK1 Mouse mAb (108A429) (Cat. No. AM76) (2 µg/ml). Detection: chemiluminescence.
      DescriptionProtein G purified mouse monoclonal antibody. Recognizes the ~80 kDa TBK1 protein.
      BackgroundNuclear factor κB (NF-κB) is sequestered in cell cytoplasm by the IκB family of inhibitory proteins that mask the nuclear localization signal of NF-κB, preventing its translocation to the nucleus. Stimulus by tumor necrosis factor or other cytokines results in the phosphorylation and degradation of IκB, releasing NF-κB dimers. These dimers then translocate to the nucleus and activate target genes. IκBα synthesis is autoregulated. IκB proteins are phosphorylated by an IκB kinase made up of at least three proteins; IKK1/α, IKK2/β, and IKK3/γ. IKK 1/α and IKK2/β are phosphorylated by NF-κB-inducing kinase (NIK) and MAP kinase kinase kinase-1 (MEKK1) respectively. TANK-binding kinase (TBK1) has recently been identified as a member of the IKK family. TRAF-associated NF-κB activator (TANK) interacts with TBK1 and activates NF-κB in a kinase-dependent manner.
      HostMouse
      Immunogen speciesHuman
      Immunogenfull-length, recombinant, human TBK1 fused to a His•Tag® sequence
      Clone108A429
      IsotypeIgG₁
      Specieshuman
      Positive controlHEK293 cells
      Negative controlNormal mouse serum
      FormLiquid
      FormulationIn PBS, 0.2% gelatin.
      Concentration Label Please refer to vial label for lot-specific concentration
      Preservative≤0.1% sodium azide
      CommentsAntibody should be titrated for optimal results in an individual systems.
      Storage Avoid freeze/thaw
      -20°C
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Toxicity Standard Handling
      ReferencesPomeerantz, J.L. and Baltimore, D. 1999. EMBO J. 18, 6694.
      Didonato, J.A., et al. 1997. Nature 388, 548.
      Lee, F.S., et al. 1997. Cell 88, 213.
      Malinin, N.L., et al. 1997. Nature 385, 540.
      Mercurio, F., et al. 1997. Science 278, 860.
      Regnier, C.H., et al. 1997. Cell 90, 373.
      Verma, I. and Stevenson, J.K. 1997. Proc. Natl. Acad. Sci. USA 94, 11758.
      Verma, I.M., et al. 1995. Genes Dev. 9, 2723.