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ABE1318 Anti-SMARCD2 (BAF60B)

Anti-SMARCD2 (BAF60B), Cat. No. ABE1318, is a highly specific rabbit polyclonal antibody that targets SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily D member 2 and has been tested for use in Immunocytochemistry, Immunohistochemistry (Paraffin), and Western Blotti

Anti-SMARCD2 (BAF60B) MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Epigenetics & Nuclear Function Gene Symbol: SMARCD2, BAF60B, PRO2451 UniProt Number: Q92925
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ABE1318
100 μg  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HICC, IH(P), WBRbAffinity PurifiedPolyclonal Antibody
      Description
      Catalogue NumberABE1318
      DescriptionAnti-SMARCD2 (BAF60B)
      Alternate Names
      • SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily D member 2
      • 60 kDa BRG-1/Brm-associated factor subunit B
      • BRG1-associated factor 60B
      • BAF60B
      Background InformationSWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily D member 2 (UniProt: Q92925; also known as 60 kDa BRG-1/Brm-associated factor subunit B, BRG1-associated factor 60B, BAF60B) is encoded by the SMARCD2 (also known as BAF60B, PRO2451) gene (Gene ID: 6603) in human. It is a member of the SWI/SNF family of proteins, whose members display helicase and ATPase activities and regulate transcription of certain genes by altering the chromatin structure around those genes. Three members of BAF60 family have been described and are designated as FAF60 A, B, and C. BAF60B is expressed in immune cells, muscle, and pancreas tissues. Two isoforms of BAF60B have been described that are produced by alternative splicing. The SWIB domain of BAF60B is localized in amino acids 307-382. BAF60B is reported to interact with multiple transcription factors and may bridge transcription factors with SWI/SNF complexes. At least 11 subunits of SWI/SNF complex have been discovered, including 2 enzymatic ATPase subunits (Brm and Brg1) and 10 Brg1/Brm associated factors (BAFs), which are named according to their molecular weights. BAF60B is shown to link chromatin opening to ATM activation by facilitating ATM recruitment to the open chromatin regions of a panel of hepatic gene loci. It is required for 3TF (transcription factors Foxa3, Hnf1 and Gata4) - induced activation of ATM and facilitates the recruitment of ATM to the SWI/ SNF complex. (Ref.: Ji, S., et al. (2017). Cell Res. 27(5); 642-656; Lores, P., et al. (2010). FEBS. J. 277(6); 1453-1464).
      References
      Product Information
      FormatAffinity Purified
      PresentationPurified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationAnti-SMARCD2 (BAF60B), Cat. No. ABE1318, is a highly specific rabbit polyclonal antibody that targets SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily D member 2 and has been tested for use in Immunocytochemistry, Immunohistochemistry (Paraffin), and Western Blotti
      Key Applications
      • Immunocytochemistry
      • Immunohistochemistry (Paraffin)
      • Western Blotting
      Application NotesWestern Blotting Analysis: 0.5 µg/mL from a representative lot detected SMARCD2 (BAF60B) in 10 µg of HeLa cell lysate.

      Immunocytochemistry Analysis: A 1:500 dilution from a representative lot detected SMARCD2 (BAF60B) in HeLa, A431, HUVEC and NIH/3T3 cell lines.

      Immunohistochemistry Analysis: A 1:1,000 dilution from a representative lot detected SMARCD2 (BAF60B) in human cerebral cortex, human testis, and human ovarian cancer tissue sections.
      Biological Information
      ImmunogenKLH-conjugated linear peptide corresponding to 13 amino acids from the N-terminal region of human SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily D member 2 (BAF60B) protein.
      EpitopeN-terminus
      ConcentrationPlease refer to lot specific datasheet.
      HostRabbit
      SpecificityThis rabbit polyclonal antibody detects SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily D member 2 (SMARCD2) in human cells. It targets an epitope within 13 amino acids from the N-terminal region.
      Species Reactivity
      • Human
      Species Reactivity NoteHuman. Predicted to react with Mouse, Rat, and Bovine based on 100% sequence homology.
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • SMARCD2
      • BAF60B
      • PRO2451
      Purification MethodAffinity Purified
      UniProt Number
      Molecular Weight~59 kDa observed; 58.92 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in Jurkat nuclear extract.

      Western Blotting Analysis: 0.5 µg/mL of this antibody detected SMARCD2 (BAF60B) in 10 µg of Jurkat cell nuclear extract.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      ABE1318 04054839415104