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ABE2613-25UL Anti-SETDB2

This rabbit polyclonal Anti-SETDB2, Cat. No. ABE2613 is tested for use in Western Blotting and Chromatin Immunoprecipitation (ChIP), Immunohistochemistry (Paraffin) for the detection of Histone-lysine N-methyltransferase SETDB2.

ABE2613-25UL
25 µL  
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      Overview

      Replacement Information
      Description
      Catalogue NumberABE2613-25UL
      DescriptionAnti-SETDB2
      Alternate Names
      • Histone-lysine N-methyltransferase SETDB2
      • Chronic lymphocytic leukemia deletion region gene 8 protein
      • Lysine N-methyltransferase 1F
      • SET domain bifurcated 2
      Background InformationHistone-lysine N-methyltransferase SETDB2 (UniProt: Q96T68; also known as Chronic lymphocytic leukemia deletion region gene 8 protein, Lysine N-methyltransferase 1F, SET domain bifurcated 2) is encoded by the SETDB2 (also known as C13orf4, CLLD8, KMT1F) gene (Gene ID: 83852) in human. SETDB2 is a member of the class V-like SAM-binding methyltransferase superfamily that is involved in the left-right axis specification in early development and mitosis. Specifically, it trimethylates lysine 9 of histone H3 (H3K9me3). H3K9me3 is a specific tag for epigenetic transcriptional repression that recruits HP1 (CBX1, CBX3 and/or CBX5) proteins to methylated histones and keeps chromatin in a conformation where the promoter is not accessible for transcription factor binding. SETDB2 is reported to contribute to H3K9me3 in both the interspersed repetitive elements and in centromere-associated repeats and plays a role in chromosome condensation and segregation during mitosis. SETDB2 is shown to be induced by STAT1 in response to type I interferon (IFN /β) signalling to suppress the expression of NF-B target genes to attenuate the inflammatory response. In wound macrophages the expression of SETDB2 is also reported to depend on type I interferon/JAK/STAT1 signaling. SETDB2 has been associated with stimulating cell proliferation, especially in cancer tissue. It is reported to promote hyperproliferation and oncogenesis in acute lymphoblastic leukemia by suppressing CDKN2C activity. (Ref.: Lin, C-H., et al. (2018). Cell Rep. 23(4); 1166-1177; Kimball, AS., et al. (2019). Immunity. 51(2); 258-271; Torrano, J., et al. (2019). Clin. Epigenet. 11; Article 43).
      References
      Product Information
      FormatSerum
      PresentationRabbit polyclonal antibody in serum with 0.05% sodium azide.
      Quality LevelMQ200
      Applications
      ApplicationThis rabbit polyclonal Anti-SETDB2, Cat. No. ABE2613 is tested for use in Western Blotting and Chromatin Immunoprecipitation (ChIP), Immunohistochemistry (Paraffin) for the detection of Histone-lysine N-methyltransferase SETDB2.
      Key Applications
      • Western Blotting
      • Chromatin Immunoprecipitation (ChIP)
      • Immunohistochemistry (Paraffin)
      Application NotesImmunohistochemistry Applications: A representative lot detected SETDB2 in Immunohistochemistry applications (Kimball, A.S., et. al. (2019). Immunity. 51(2):258-271).

      Immunohistochemistry (Paraffin) Analysis: A 1:1,000 dilution from a representative lot detected SETDB2 in mouse testis tissue sections.

      Western Blotting Analysis: A representative lot detected SETDB2 in Western Blotting applications (Kimball, A.S., et. al. (2019). Immunity. 51(2):258-271).

      Chromatin Immunoprecipitation Analysis (ChIP): A representative lot detected SETDB2 in Chromatin Immunoprecipitation applications (Kimball, A.S., et. al. (2019). Immunity. 51(2):258-271).

      Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
      Biological Information
      ImmunogenHis-tagged recombinant fragment corresponding to 107 amino acids from the N-terminal region of human Histone-lysine N-methyltransferase SETDB2.
      EpitopeUnknown
      ConcentrationPlease refer to guidance on suggested starting dilutions and/or titers per application and sample type.
      HostRabbit
      SpecificityThis rabbit polyclonal antibody specifically detects Histone-lysine N-methyltransferase SETDB2. It targets an epitope within the N-terminal region.
      Species Reactivity
      • Human
      • Mouse
      Species Reactivity NoteHuman, Mouse.
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • SETDB2
      • C13orf4
      • CLLD8
      • KMT1F
      Purification MethodUnpurified
      UniProt Number
      Molecular Weight~80 kDa observed; 81.89 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in human heart tissue lysate.

      Western Blotting Analysis: A 1:1,000 dilution of this antibody detected SETDB2 in human heart tissue lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -10°C to -25°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
      Packaging Information
      Material Size25 µL
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      ABE2613-25UL 04065265398121