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ABN327 Anti-SCRAPPER Antibody

This SCRAPPER antibody is validated for use in WB & IHC for the detection of the SCRAPPER protein.

Anti-SCRAPPER Antibody MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Neuroscience Research Sub-Category: Developmental Neuroscience Gene Symbol: FBXL20, SCRAPPER UniProt Number: Q96IG2
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ABN327
100 µg  
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      Overview

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      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HWB, IHCRbAffinity PurifiedPolyclonal Antibody
      Description
      Catalogue NumberABN327
      DescriptionAnti-SCRAPPER Antibody
      Alternate Names
      • F-box
      • Leucine rich repeat protein 20
      • FBXL20
      • FBl2
      • FBL20
      Background InformationMembers of the F-box protein family, such as SCRAPPER, are characterized by an approximately 40-amino acid F-box motif. SCRAPPER is selectively expressed in the brain, broadly expressed within the mouse CNS, and is abundant at the presynaptic membrane. It has orthologs in C. elegans, D. melanogaster, and mammals which suggests that it might function as an important membrane-localized E3 ligase in various species. SCRAPPER is a major presynaptic E3 ubiquitin ligase that acts through RIM1a via degradation and the ubiquitin-proteasome-system (UPS) pathway to critically regulate synaptic transmission. This identifies protein degradation as a mechanism for holding synaptic communication in check.
      References
      Product Information
      FormatAffinity Purified
      Control
      • Human spleen tissue lysate
      PresentationPurified rabbit polyclonal in buffer containing PBS with 0.02% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationThis SCRAPPER antibody is validated for use in WB & IHC for the detection of the SCRAPPER protein.
      Key Applications
      • Western Blotting
      • Immunohistochemistry
      Application NotesWestern Blot Analysis: 1 µg/mL from a representative lot detected SCRAPPER in 10 µg/mL of RAW264.7 cell lysate.

      Immunohistochemistry Analysis: A 1:500 dilution from a representative lot detected SCRAPPER in human midbrain tissue.
      Biological Information
      ImmunogenKLH-conjugated linear peptide corresponding to human SCRAPPER.
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostRabbit
      Species Reactivity
      • Human
      Antibody TypePolyclonal Antibody
      Gene Symbol
      • FBXL20
      • SCRAPPER
      Purification MethodAntigen Affinity Purified
      UniProt Number
      Molecular Weight~48 kDa observed. Uncharacterized bands may appear at ~52 kDa, ~58 kDa and ~70 kDa in some lysates.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blot in human spleen tissue lysate.

      Western Blot Analysis: 1 µg/mL of this antibody detected SCRAPPER in 10 µg/mL of human spleen tissue lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      ABN327 04053252831096