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MABS1249 Anti-SCAP Antibody, clone 9D5

Anti-SCAP Antibody, clone 9D5 is an antibody against SCAP for use in Western Blotting, Immunocytochemistry.

Anti-SCAP Antibody, clone 9D5 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Signaling Research Sub-Category: Lipid Signaling Gene Symbol: SCAP UniProt Number: P97260
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MABS1249
100 μg  
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      Overview

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      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HtWB, ICCMPurifiedMonoclonal Antibody
      Description
      Catalogue NumberMABS1249
      DescriptionAnti-SCAP Antibody, clone 9D5
      Alternate Names
      • Sterol regulatory element-binding protein cleavage-activating protein
      • SCAP
      • SREBP cleavage-activating protein
      Background InformationSterol regulatory element-binding protein cleavage-activating protein (UniProt P97260; also known as SCAP, SREBP cleavage-activating protein) is encoded by the SCAP gene (Gene ID 100689048) in hamster species. SCAP mediates the activation of membrane-bound transcription factors SREBPs (sterol-regulatory element binding proteins) for transcribing genes encoding cholesterol biosynthetic enzymes. In sterol-deprived cells, SCAP escorts SREBPs from ER to Golgi, where active fragments of SREBPs are released from membrane by proteolysis. Sterol accumulation inuduces a negative regulatory mechanism, where SCAP becomes bound by Insigs and trapped in the ER, preventing further delivery of SREBPs to the Golgi for proteolytic release. SCAP spans the ER membrane eight times (a.a. 19-39, 280-300, 313-333, 345-365, 402-422, 424-444, 519-539, 709-729) with
      4 lumenal and 5 cytoplasmic regions, having both its N- and C-terminal ends at the cytoplasmic side (a.a. 1-18 & 730-1276). The C-terminal domain of SCAP mediates association with SREBPs, while transmembrane helices 2–6 comprise the sterol-sensing domain that mediates sterol-induced binding of SCAP to Insigs. Mutations within the sterol-sensing region disrupt Insig binding and prevent sterol-mediated ER retention of SCAP-SREBP.
      References
      Product Information
      FormatPurified
      PresentationPurified mouse monoclonal IgG2bκ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationAnti-SCAP Antibody, clone 9D5 is an antibody against SCAP for use in Western Blotting, Immunocytochemistry.
      Key Applications
      • Western Blotting
      • Immunocytochemistry
      Application NotesWestern Blotting Analysis: 5 µg/mL from a representative lot detected SCAP in 25 µg of whole cell lysate and 50 µg of membrane extract from CHO-K1 cells, but not from the SCAP-deficient SRD-13A CHO cells (Courtesy of Linda Donnelly, Department of Molecular Genetics, UT Southwestern Medical Center, Dallas, TX, USA).
      Western Blotting Analysis: A representative lot detected exogenously expressed SCAP co-immunoprecipitated with the exogenously expressed Insig-1 and Insig-2 upon 25-hydroxycholesterol (25-HC) treatment of a SCAP-deficient CHO cell line SRD-13A that had been transfected to co-express SCAP with either myc-tagged Insig-1 or Insig-2 (Lee, P.C., and DeBose-Boyd, R.A. (2010). J. Lipid Res. 51(1):192-201).
      Western Blotting Analysis: Representative lots detected similar level of SCAP in the membrane extracts from CHO cells with or without SREBP processing inhibitor 25-hydroxycholesterol (25-HC) treatment regardless whether the cells' 25-HC-resistant status (Lee, P.C., and DeBose-Boyd, R.A. (2010). J. Lipid Res. 51(1):192-201; Lee, P.C., et al. (2007). J. Lipid Res. 48(9):1944-1954).
      Western Blotting Analysis: A representative lot detected the endogenous SCAP immunoprecipitated from CHO cell lysate by a polyclonal SCAP antibody (Sakai, J., et al. (1997). J. Biol. Chem. 272(32):20213-20221).
      Immunocytochemistry Analysis: A representative lot localized the overexpressed hamster SCAP at ER and nuclear envelope by fluorescent immunocytochemistry staining of 3% paraformaldehyde-fixed, 0.01% saponin-permeabilized CHO stable transfectants (Sakai, J., et al. (1997). J. Biol. Chem. 272(32):20213-20221).
      Biological Information
      ImmunogenHis-tagged recombinant fragment corresponding to the fourth lumenal domain of hamster SCAP.
      EpitopeThe fourth lumenal domain.
      Clone9D5
      ConcentrationPlease refer to lot specific datasheet.
      HostMouse
      SpecificityClone 9D5 detected the target band in lysate and membrane extract from CHO-K1 cells, but not from the SCAP-deficient SRD-13A CHO cell line.
      IsotypeIgG2bκ
      Species Reactivity
      • Hamster
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • SCAP
      Purification MethodProtein G purified.
      UniProt Number
      Molecular Weight~150 kDa observed. Target band size appears larger than the calculated molecular weight of 139.5 kDa due to glycosylation. Uncharacterized band(s) may appear in some lysates.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceIdentity Confirmation by Isotyping Test.

      Isotyping Analysis: The identity of this monoclonal antibody is confirmed by isotyping test to be IgG2bκ.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      MABS1249 04055977351019