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05-777 Anti-Phosphotyrosine Antibody, recombinant clone 4G10®

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05-777
1 mg  
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      Overview

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      VrtIP, WBMPurifiedMonoclonal Antibody
      Description
      Catalogue Number05-777
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionAnti-Phosphotyrosine Antibody, recombinant clone 4G10®
      Background InformationSome of the tyrosine residues can be tagged with a phosphate group (phosphorylated) by protein kinases. (In its phosphorylated state, it is referred to as phosphotyrosine.). Tyrosine phosphorylation is considered as one of the key steps in signal transduction and regulation of enzymatic activity. The advent of anti-phospho-tyrosine antibodies is one of significant events in signal transduction research. Before the availability of anti-phosphotyrosine antibodies, tyrosyl phospyhorylation of proteins and enzymes was investigated through hazardous and time-consuming radioactive experiments. Anti-phosphotyrosine antibodies are commonly used in western blots after the targeted proteins have been immunoprecipitated to measure the tyrosyl phosphorylation of the proteins. Anti-phosphotyrosine antibodies are also directly used on cell lysate to examine the overall change of tyrosine phosphorylation level in reponse to various treatments.
      Product Information
      FormatPurified
      Control
      • Positive Control Included: EGF stim A431 Cell Lysate (12-302).
      PresentationProtein G purified recombinant 4G10 mouse IgG2bk in 0.01M Phosphate Buffer containing no preservative, pH 7.1
      Quality LevelMQ100
      Applications
      ApplicationAnti-Phosphotyrosine Antibody, clone 4G10 is a recombinant antibody that detects tyrosine phosphorylated proteins in all species. This unique recombinant antibody is validated for use in IC, IH, IP, WB and is published in peer review journals.
      Key Applications
      • Immunoprecipitation
      • Western Blotting
      Biological Information
      ImmunogenProduced from CHO cells expressing the 4G10 antibody heavy and light chain cDNAs. Heavy chain C-terminus has a hexa-histidine tag for purification and immobilization via Nickel affinity matrices.
      Clone4G10®
      HostMouse
      SpecificityRecognizes tyrosine-phosphorylated proteins from all species.
      IsotypeIgG2bκ
      Species Reactivity
      • Vertebrates
      Antibody TypeMonoclonal Antibody
      Modifications
      • Phosphorylation
      Purification MethodProtein G Purified
      Molecular Weightvaries
      Product Usage Statements
      Quality Assuranceroutinely evaluated by immunoblot on a modified RIPA lysate from EGF-treated human A431 carcinoma cells
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsMaintain for 2 years at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
      Packaging Information
      Material Size1 mg
      Global Trade Item Number
      Catalogue Number GTIN
      05-777 04053252371110