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ABS517 Anti-PRMT8 Antibody

This Anti-PRMT8 Antibody is validated for use in Western Blotting and Immunohistochemistry for the detection of PRMT8.

Anti-PRMT8 Antibody MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Epigenetics & Nuclear Function, Epigenetics & Nuclear Function Research Sub-Category: Chromatin Biology, Histones Gene Symbol: PRMT8, HRMT1L3, HRMT1L4 UniProt Number: Q9NR22
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ABS517
100 µg  
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      Overview

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      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, MWB, IHCRbAffinity PurifiedPolyclonal Antibody
      Description
      Catalogue NumberABS517
      DescriptionAnti-PRMT8 Antibody
      Alternate Names
      • Protein arginine N-methyltransferase 8
      • Heterogeneous nuclear ribonucleoprotein methyltransferase-like protein 4
      • Protein arginine methyltransferase 8
      Background InformationAsymmetric dimethylation of arginine side chains in proteins is a frequent posttranslational modification, catalyzed by type I protein arginine methyltransferases (PRMTs). PRMT8 is a membrane-associated arginine methyltransferase that can both catalyze the formation of omega-N monomethylarginine (MMA) and asymmetrical dimethylarginine (aDMA). PRMT8 can form dimers with PRMT2 or PRMT1 and interacts with FYN kinase. PRMT8 is brain specific and it is a vertebrate restricted paralogue of PRMT1. In the past, arginine methylation was mainly observed on abundant proteins such as RNA-binding proteins and histones, but recent advances have revealed a plethora of arginine-methylated proteins implicated in a variety of cellular processes including signal transduction, epigenetic regulation and DNA repair pathways. PRMT8 has been shown to be critical for proper neuronal development and its expression is regionally restricted in the adult suggesting that PRMT8 is chiefly involved in the somatosensory and limbic systems, and a part of the motor nervous system development and maintenance.
      References
      Product Information
      FormatAffinity Purified
      PresentationPurified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationThis Anti-PRMT8 Antibody is validated for use in Western Blotting and Immunohistochemistry for the detection of PRMT8.
      Key Applications
      • Western Blotting
      • Immunohistochemistry
      Application NotesImmunohistochemistry Analysis: A 1:500 dilution from a representative lot detected PRMT8 in human and mouse brain tissuse, and mouse thalamus.
      Biological Information
      ImmunogenKLH-conjugated linear peptide corresponding to the SAM-dependent methyltransferase PRMT-type domain of Human PRMT8.
      EpitopeC-terminal region of SAM-dependent methyltransferase PRMT-type domain
      ConcentrationPlease refer to lot specific datasheet.
      HostRabbit
      Species Reactivity
      • Human
      • Mouse
      Species Reactivity NoteHuman. Predicted to react with Canine, Bovine, Porcine, Chicken, Baboon based on 100% sequence homology. Other homologies: Homology: predicted to react with rat.
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • PRMT8
      • HRMT1L3
      • HRMT1L4
      Purification MethodAntigen Affinity Purified
      UniProt Number
      Molecular Weight~48 kDa observed
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting using human fetal brain tissue lysate.

      Western Blotting Analysis: 0.1 µg/mL of this antibody detected PRMT8 in 10 µg of human fetal brain tissue lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      ABS517 04053252983726