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ST1508 Anti-PCK1 Mouse mAb (3E4)

This Anti-PCK1 Mouse mAb (3E4) is validated for use in ELISA, Immunoblotting, Immunocytochemistry for the detection of PCK1.

Anti-PCK1 Mouse mAb (3E4) MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: Anti-Phosphenolpyruvate Carboxykinase 1

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ST1508
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Overview

Replacement Information

Key Spec Table

Species ReactivityHostAntibody Type
HMMonoclonal Antibody

Pricing & Availability

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ST1508-100UGCN
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      		 100 μg
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      Description
      OverviewRecognizes the ~70 kDa PCK1 protein in HepG2 cells.
      Catalogue NumberST1508
      Brand Family Calbiochem®
      SynonymsAnti-Phosphenolpyruvate Carboxykinase 1
      Application Data
      Detection of human PCK1 by immunoblotting. Sample: Whole cell lysate from HepG2 cells. Primary antibody: Anti-PCK1 Mouse mAb (3E4) (Cat. No. ST1508) (1-5 µg/ml). Detection: chemiluminescence.

      Detection of human PCK1 by immunocytochemistry. Sample: HepG2 cells fixed in paraformaldehyde. Primary antibody: Anti-PCK1 Mouse mAb (3E4) (Cat. No. ST1508) (10 µg/ml). Detection: fluorescence.
      References
      Product Information
      FormLiquid
      FormulationIn PBS, pH 7.2.
      Negative control293T cells
      Positive controlHepG2 cells
      PreservativeNone
      Quality LevelMQ100
      Applications
      Key Applications Enzyme-Linked Immunosorbent Assay
      Immunoblotting (Western Blotting)
      Immunofluorescence
      Application NotesELISA (see comments)
      Immunoblotting (1-5 µg/ml)
      Immunocytochemistry (10 µg/ml)
      Application CommentsVariables associated with assay conditions will dictate the proper working dilution.
      Biological Information
      ImmunogenFull-length, recombinant human PCK1 (aa 1-623), expressed as a GST fusion protein
      Clone3E4
      HostMouse
      IsotypeIgG₁
      Species Reactivity
      • Human
      Antibody TypeMonoclonal Antibody
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Blue Ice Only
      Toxicity Regulatory Review
      Storage -20°C
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      ST1508-100UGCN 04055977224160

      Documentation

      Anti-PCK1 Mouse mAb (3E4) SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-PCK1 Mouse mAb (3E4) Certificates of Analysis

      TitleLot Number
      ST1508
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision05-November-2010 RFH
      SynonymsAnti-Phosphenolpyruvate Carboxykinase 1
      ApplicationELISA (see comments)
      Immunoblotting (1-5 µg/ml)
      Immunocytochemistry (10 µg/ml)
      Application Data
      Detection of human PCK1 by immunoblotting. Sample: Whole cell lysate from HepG2 cells. Primary antibody: Anti-PCK1 Mouse mAb (3E4) (Cat. No. ST1508) (1-5 µg/ml). Detection: chemiluminescence.

      Detection of human PCK1 by immunocytochemistry. Sample: HepG2 cells fixed in paraformaldehyde. Primary antibody: Anti-PCK1 Mouse mAb (3E4) (Cat. No. ST1508) (10 µg/ml). Detection: fluorescence.
      DescriptionPurified mouse monoclonal antibody. Recognizes the ~70 kDa PCK1 protein.
      BackgroundPCK1 is a main control point for the regulation of gluconeogenesis. The cytosolic enzyme encoded by this gene, along with GTP, catalyzes the formation of phosphoenolpyruvate from oxaloacetate, with the release of carbon dioxide and GDP. The expression of this gene can be regulated by insulin, glucocorticoids, glucagon, cAMP, and diet. Defects in this gene are a cause of cytosolic phosphoenolpyruvate carboxykinase deficiency.
      HostMouse
      ImmunogenFull-length, recombinant human PCK1 (aa 1-623), expressed as a GST fusion protein
      Clone3E4
      IsotypeIgG₁
      Specieshuman
      Positive controlHepG2 cells
      Negative control293T cells
      FormLiquid
      FormulationIn PBS, pH 7.2.
      PreservativeNone
      CommentsVariables associated with assay conditions will dictate the proper working dilution.
      Storage Avoid freeze/thaw
      -20°C
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Toxicity Regulatory Review