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AB5407 Anti-Opsin Antibody, blue

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AB5407
100 µg  
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      Overview

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, M, MkIH(P)RbPurifiedPolyclonal Antibody
      Description
      Catalogue NumberAB5407
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionAnti-Opsin Antibody, blue
      Background InformationThe full range of color discrimination in humans is based on the presence and function of three cone photoreceptor mechanisms. Each cone type possesses a photo-sensitive pigment-protein complex consisting of 11-cis retinal and a unique opsin protein, which gives sensitivity in the short (S cone, peak sensitivity about 420nm), middle (M cone, peak sensitivity about 530nm with polymorphism; Winderckx et al., 1993; Neitz & Neitz, 1998), and long (L cone, peak sensitivity about 560nm with polymorphism; Neitz & Jacobs, 1990) wavelengths of the light spectrum. All three opsins are transmembrane proteins with seven membrane-spanning regions. Genes for the three types of cone opsins and the rod photoreceptor rhodopsin gene seem to be homologous with varying amounts of conservation. Strongest conservation is between the middle (green) and long (red) wavelength sensitive pigments on the X chromosome, suggesting a relatively recent duplication/divergence event (Nathans, 1989; Nathans et al., 1992). The S cone (blue) opsin is located on chromosome 7 and seems to have stronger conservation with rhodopsin. Cone photoreceptor distribution in humans is dominated by the M and L cone pigments.
      Product Information
      FormatPurified
      HS Code3002 15 90
      Control
      • Retina
      PresentationPurified immunoglobulinin PBS {0.02M phosphate, 0.25M NaCl, pH 7.6} with 0.1% sodium azide as a preservative
      Quality LevelMQ100
      Applications
      ApplicationThis Anti-Opsin Antibody, blue is validated for use in IH(P) for the detection of Opsin.
      Key Applications
      • Immunohistochemistry (Paraffin)
      Application NotesImmunohistochemistry: 1:200-1:300 on formalin-fixed, paraffin-embedded mouse retina tissue. Antigen retrieval method recommend is HIER with steam heat; other fixation and retrieval methods are untested.

      Optimal working dilutions must be determined by the end user.
      Biological Information
      ImmunogenRecombinant human blue opsin.
      Epitopeblue
      HostRabbit
      SpecificityRecognizes Opsin, blue.
      Species Reactivity
      • Human
      • Mouse
      • Monkey
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • OPN1SW
      • CBT
      • BOP
      • BCP
      Purification MethodProtein A Purfied
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P03999 # Visual pigments are the light-absorbing molecules that mediate vision. They consist of an apoprotein, opsin, covalently linked to cis-retinal.
      SIZE: 348 amino acids; 39135 Da
      SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein.
      TISSUE SPECIFICITY: The three color pigments are found in the cone photoreceptor cells.
      PTM: Phosphorylated on some or all of the serine and threonine residues present in the C-terminal region.
      DISEASE:SwissProt: P03999 # Defects in OPN1SW are the cause of tritan color blindness (tritanopia) [MIM:190900].SIMILARITY:SwissProt: P03999 ## Belongs to the G-protein coupled receptor 1 family. Opsin subfamily.
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsMaintain for 1 year at 2–8°C from date of shipment.
      Packaging Information
      Material Size100 µg
      Global Trade Item Number
      Catalogue Number GTIN
      AB5407 04053252468544