Millipore Sigma Vibrant Logo

AB5583-50UL Anti-Nerve Growth Factor Antibody, pro

This Anti-Nerve Growth Factor Antibody, pro is validated for use in WB, IH for the detection of Nerve Growth Factor.

Anti-Nerve Growth Factor Antibody, pro MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Neuroscience Research Sub-Category: Neurochemistry & Neurotrophins Gene Symbol: NTF3, HDNF, NGF-2, NT3, NGF2, MGC129711, NT-3 UniProt Number: P20783
View Products on Sigmaaldrich.com
AB5583-50UL
50 µL  
Price could not be retrieved
Minimum Quantity is a multiple of
Maximum Quantity is
Upon Order Completion More Information
You Saved ()
 
—
Request Pricing
—
Limited Availability Limited Availability
In Stock 
Discontinued
Limited Quantities Available
Availability to be confirmed
    Remaining : Will advise
      Remaining : Will advise
      Will advise
      Contact Customer Service
      Contact Customer Service

      Special Offers

       

      Contact Customer Service

      Overview

      Special Offers

      100% Performance Guaranteed

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      RWB, IHCRbAffinity PurifiedPolyclonal Antibody
      Description
      Catalogue NumberAB5583-50UL
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionAnti-Nerve Growth Factor Antibody, pro
      Alternate Names
      • NGF, pro
      Product Information
      FormatAffinity Purified
      Control
      • Included free of charge with the antibody is 40 μg of control antigen (lyophilized powder). The stock solution of the antigen can be made up using 100 μL of sterile distilled water. For negative control, preincubate 1 μg of purified peptide with 1 μg of antibody for one hour at room temperature. Optimal concentrations must be determined by the end user.
      PresentationAffinity purified immunoglobulin. Lyophilized from PBS, pH 7.4, containing 1% BSA and 0.025% sodium azide. Reconstitute with 50 mL of sterile distilled water. Centrifuge antibody preparation before use (10,000 x g for 5 min).
      Quality LevelMQ100
      Applications
      ApplicationThis Anti-Nerve Growth Factor Antibody, pro is validated for use in WB, IH for the detection of Nerve Growth Factor.
      Key Applications
      • Western Blotting
      • Immunohistochemistry
      Application NotesWestern blotting: 1:200-1:500 using ECL on His-fusion protein containing the entire prodomain of rat proNGF.

      Immunohistochemistry on rat brain sections.

      Dilutions should be made using a carrier protein such as BSA (1-3%).

      Optimal working dilutions must be determined by the end user.
      Biological Information
      ImmunogenSynthetic corresponding to amino acids 84-104 of rat proNGF (Accession P25427).
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostRabbit
      SpecificityRecognizes proNGF (Nerve Growth Factor).

      SPECIES REACTIVITIES:

      The immunogen sequence has 100% homology with mouse and 76% homology with human proNGF. Reactivity with other species has not yet been tested.
      Species Reactivity
      • Rat
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThe protein encoded by this gene is a member of the neurotrophin family, that controls survival and differentiation of mammalian neurons. This protein is closely related to both nerve growth factor and brain-derived neurotrophic factor. It may be involved in the maintenance of the adult nervous system, and may affect development of neurons in the embryo when it is expressed in human placenta. NTF3-deficient mice generated by gene targeting display severe movement defects of the limbs. The mature peptide of this protein is identical in all mammals examined including human, pig, rat and mouse.
      Gene Symbol
      • NTF3
      • HDNF
      • NGF-2
      • NT3
      • NGF2
      • MGC129711
      • NT-3
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P20783 # Seems to promotes the survival of visceral and proprioceptive sensory neurons.
      SIZE: 257 amino acids; 29355 Da
      SUBCELLULAR LOCATION: Secreted.
      TISSUE SPECIFICITY: Brain and peripheral tissues.
      SIMILARITY: SwissProt: P20783 ## Belongs to the NGF-beta family.
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsMaintain lyophilized material at -20°C for up to 6 months after date of receipt. After reconstitution maintain antibody and control peptide at -20°C in undiluted aliquots for up to 6 months. Avoid repeated freeze/thaw cycles.
      Packaging Information
      Material Size50 µL
      Global Trade Item Number
      Catalogue Number GTIN
      AB5583-50UL 04053252588389