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MAB2077Z Anti-Integrin αVβ6 Antibody, clone 10D5, azide free

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MAB2077Z
100 µg  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, M, RFC, IP, FUNCMPurifiedMonoclonal Antibody
      Description
      Catalogue NumberMAB2077Z
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionAnti-Integrin αVβ6 Antibody, clone 10D5, azide free
      Alternate Names
      • MAB2077
      Background InformationThe integrin family of cell adhesion receptors consists of at least 19 membrane-associated heterodimers, composed of an alpha and beta subunit that associate in a non-covalent manner. To date, 16 different alpha-subunits and 8 different beta-subunits have been identified. The structure and functional diversity of the integrin family are based upon the pairing abilities of the individual alpha and beta subunits. Key to these molecular interactions between the integrin receptors and their respective ligands is the recognition of the Arg-Gly-Asp (RGD) sequence, known to be present in the extracellular matrix components fibronectin, vitronectin, collagen, fibrinogen, and von Willebrand factor (Cheresh, 1991). The involvement of integrins in vascular proliferation, adhesion, and wound repair has been well documented. Integrin alphaV is a type I membrane glycoptroein with no I (inserted) domain. The alphaV protein is post-translationally cleaved into a heavy and light chain, which is expressed on the cell surface as a disulfide linked polypeptide.

      The integrin alphavbeta6 is expressed on a variety of epithelial cells during dynamic processes including organogenesis, tissue injury and malignant transformation.
      References
      Product Information
      FormatPurified
      Control
      • HT-29 colon carcinoma cells
      PresentationProtein A Purified mouse immunoglobulin in 20 mM sodium phosphate, 250 mM NaCl, pH. 7.6, with no preservatives.
      Quality LevelMQ100
      Applications
      ApplicationAnti-Integrin αVβ6 Antibody, clone 10D5, azide free is an antibody against Integrin αVβ6 for use in FC & IP.
      Key Applications
      • Flow Cytometry
      • Immunoprecipitation
      • Affects Function
      Applications Not Recommended
      • Western Blotting
      • Immunohistochemistry
      Application NotesFlow cytometry: Effective for mouse and human.

      Immunoprecipitation: Effective; however because this antibody is an IgG2a, protein G or rabbit anti-mouse IgG is helpful for capture

      Function blocking: 100% inhibition of alphaVbeta6-mediated cell adhesion and cell migration are achieved at a concentration of 100 μg/mL.

      Not effective for western blot or immunohistochemistry.

      Optimal working dilutions must be determined by end user.
      Biological Information
      ImmunogenImmunization of beta 6 knockout mice with wild type murine keratinocytes.
      Clone10D5
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostMouse
      SpecificityMonoclonal antibody MAB2077Z, raised by immunizing beta6 knockout mice with wild type murine keratinocytes, recognizes human and murine alphaVbeta6 by flow cytometry and immunoprecipitation and has blocking activity against murine and human alphaVbeta6. 100% inhibition of alphaVbeta6-mediated cell adhesion and cell migration are achieved at a concentration of <10 μg/mL. Because the antibody was raised in knockout mice, it is likely to recognize the receptor in other species, however it has been evaluated only in human and mouse.The antibody is specific for alphaVbeta6 and does not recognize aV or other aV integrins.
      IsotypeIgG2a
      Species Reactivity
      • Human
      • Mouse
      • Rat
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryITAGV encodes integrin alpha chain V. Integrins are heterodimeric integral membrane proteins composed of an alpha chain and a beta chain. The I-domain containing integrin alpha V undergoes post-translational cleavage to yield disulfide-linked heavy and light chains, that combine with multiple integrin beta chains to form different integrins. Among the known associating beta chains (beta chains 1,3,5,6, and 8; 'ITGB1', 'ITGB3', 'ITGB5', 'ITGB6', and 'ITGB8'), each can interact with extracellular matrix ligands; the alpha V beta 3 integrin, perhaps the most studied of these, is referred to as the Vitronectin receptor (VNR). In addition to adhesion, many integrins are known to facilitate signal transduction.
      Gene Symbol
      • ITGAV
      • DKFZp686A08142
      • MSK8
      • CD51
      • VNRA
      Purification MethodProtein A Purfied
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P06756 # The alpha-V integrins are receptors for vitronectin, cytotactin, fibronectin, fibrinogen, laminin, matrix metalloproteinase-2, osteopontin, osteomodulin, prothrombin, thrombospondin and von Willebrand factor. They recognize the sequence R-G-D in a wide array of ligands. In case of HIV-1 infection, the interaction with extracellular viral Tat protein seems to enhance angiogenesis in Kaposi's sarcoma lesions.
      SIZE: 1048 amino acids; 116038 Da
      SUBUNIT: Heterodimer of an alpha and a beta subunit. The alpha subunit is composed of an heavy and a light chain linked by a disulfide bond. Alpha-V associates with either beta-1, beta-3, beta-5, beta-6 or beta-8 subunit. Interacts with HIV-1 Tat.
      SUBCELLULAR LOCATION: Membrane; Single-pass type I membrane protein.
      SIMILARITY: SwissProt: P06756 ## Belongs to the integrin alpha chain family. & Contains 7 FG-GAP repeats.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsMaintain for 1 year at 2–8°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      MAB2077Z 04053252359415

      Documentation

      Anti-Integrin αVβ6 Antibody, clone 10D5, azide free SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-Integrin αVβ6 Antibody, clone 10D5, azide free Certificates of Analysis

      TitleLot Number
      MOUSE ANTI-INTEGRIN aVB6 MONOCLONAL ANTIBODY 2926173
      MOUSE ANTI-INTEGRIN aVB6 MONOCLONAL ANTIBODY 2914966
      MOUSE ANTI-INTEGRIN aVB6 Monoclonal Antibody 2890991
      MOUSE ANTI-INTEGRIN aVb6 MONOCLONAL ANTIBODY - 1991261 1991261
      MOUSE ANTI-INTEGRIN aVb6 MONOCLONAL ANTIBODY - 2034703 2034703
      MOUSE ANTI-INTEGRIN aVb6 MONOCLONAL ANTIBODY - 2090925 2090925
      MOUSE ANTI-INTEGRIN aVb6 MONOCLONAL ANTIBODY - 2207174 2207174
      MOUSE ANTI-INTEGRIN αVβ6 - 2530960 2530960
      MOUSE ANTI-INTEGRIN αVβ6 - 3439727 3439727
      MOUSE ANTI-INTEGRIN αVβ6 - 3474162 3474162

      References

      Reference overviewPub Med ID
      Altered microenvironment promotes progression of preinvasive breast cancer: myoepithelial expression of αvβ6 integrin in DCIS identifies high-risk patients and predicts recurrence.
      Allen, MD; Thomas, GJ; Clark, S; Dawoud, MM; Vallath, S; Payne, SJ; Gomm, JJ; Dreger, SA; Dickinson, S; Edwards, DR; Pennington, CJ; Sestak, I; Cuzick, J; Marshall, JF; Hart, IR; Jones, JL
      Clinical cancer research : an official journal of the American Association for Cancer Research  20  344-57  2014

      Show Abstract
      24150233 24150233
      Roles of the putative integrin-binding motif of the human metapneumovirus fusion (f) protein in cell-cell fusion, viral infectivity, and pathogenesis.
      Wei, Y; Zhang, Y; Cai, H; Mirza, AM; Iorio, RM; Peeples, ME; Niewiesk, S; Li, J
      Journal of virology  88  4338-52  2014

      Show Abstract
      24478423 24478423
      Dual inhibition of αV integrins and Src kinase activity as a combination therapy strategy for colorectal cancer.
      Jia, J; Starodub, A; Cushman, I; Liu, Y; Marshall, DJ; Hurwitz, HI; Nixon, AB
      Anti-cancer drugs  24  237-50  2013

      Show Abstract
      23275294 23275294
      Tailored integrin-extracellular matrix interactions to direct human mesenchymal stem cell differentiation.
      Frith, JE; Mills, RJ; Hudson, JE; Cooper-White, JJ
      Stem cells and development  21  2442-56  2012

      Show Abstract
      22455378 22455378
      Chemically programmed antibodies targeting multiple alpha(v) integrins and their effects on tumor-related functions in vitro.
      Goswami, RK; Bajjuri, KM; Forsyth, JS; Das, S; Hassenpflug, W; Huang, ZZ; Lerner, RA; Felding-Habermann, B; Sinha, SC
      Bioconjugate chemistry  22  1535-44  2011

      Show Abstract
      21774545 21774545
      Human cytomegalovirus induces TGF-β1 activation in renal tubular epithelial cells after epithelial-to-mesenchymal transition.
      Shimamura, M; Murphy-Ullrich, JE; Britt, WJ
      PLoS pathogens  6  e1001170  2010

      Show Abstract Full Text Article
      21079788 21079788
      Integrin alphaVbeta6 is a high-affinity receptor for coxsackievirus A9.
      Heikkilä, O; Susi, P; Stanway, G; Hyypiä, T
      The Journal of general virology  90  197-204  2009

      Show Abstract
      19088289 19088289
      Anti-integrin monoclonal antibody CNTO 95 enhances the therapeutic efficacy of fractionated radiation therapy in vivo.
      Ning, S; Nemeth, JA; Hanson, RL; Forsythe, K; Knox, SJ
      Molecular cancer therapeutics  7  1569-78  2008

      Show Abstract
      18566227 18566227
      Phenotypic and genetic characterization of a novel phenotype in pigs characterized by juvenile hairlessness and age dependent emphysema.
      Bruun, CS; Jørgensen, CB; Bay, L; Cirera, S; Jensen, HE; Leifsson, PS; Nielsen, J; Christensen, K; Fredholm, M
      BMC genomics  9  283  2008

      Show Abstract Full Text Article
      18549483 18549483
      A peptide selected by biopanning identifies the integrin alphavbeta6 as a prognostic biomarker for nonsmall cell lung cancer.
      Elayadi, AN; Samli, KN; Prudkin, L; Liu, YH; Bian, A; Xie, XJ; Wistuba, II; Roth, JA; McGuire, MJ; Brown, KC
      Cancer research  67  5889-95  2007

      Show Abstract
      17575158 17575158

      Data Sheet

      Title
      MOUSE ANTI-INTEGRIN alphaVbeta6

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies