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MAB1767 Anti-Human MT1-MMP catalytic domain Antibody, clone 3G4.2

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MAB1767
100 µg  
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      Overview

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HWB, ICC, IHCMPurifiedMonoclonal Antibody
      Description
      Catalogue NumberMAB1767
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionAnti-Human MT1-MMP catalytic domain Antibody, clone 3G4.2
      Alternate Names
      • MMP-14
      Background InformationProteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. However, MMP-14 is a member of the membrane-type MMP (MT-MMP) subfamily; each member of this subfamily contains a potential transmembrane domain suggesting that these proteins are expressed at the cell surface rather than secreted. MT1-MMP is capable of mediating pericellular proteolysis of extracellular matrix components and is therefore thought to be an important molecular tool for cellular remodeling of the surrounding matrix. This protein also activates MMP2 protein, and this activity may be involved in tumor invasion.
      Product Information
      FormatPurified
      PresentationProtein A purified immunoglobulin liquid in PBS, pH 7.2, containing 0.1% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationDetect Human MT1-MMP catalytic domain using this Anti-Human MT1-MMP catalytic domain Antibody, clone 3G4.2 validated for use in WB, IC, IH.
      Key Applications
      • Western Blotting
      • Immunocytochemistry
      • Immunohistochemistry
      Application NotesSuggested dilutions:
      Western blot: 1:1000 - 1:2000
      Biological Information
      ImmunogenRecombinant protein containing the catalytic domain of human MT1-MMP.
      Epitopecatalytic domain
      Clone3G4.2
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostMouse
      SpecificityClone 3G4.2 recognizes the catalytic domain of MT1-MMP, identifying the active form of the protein. MW is approximately 60 kDa.
      IsotypeIgG2bκ
      Species Reactivity
      • Human
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryProteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. However, the protein encoded by this gene is a member of the membrane-type MMP (MT-MMP) subfamily; each member of this subfamily contains a potential transmembrane domain suggesting that these proteins are expressed at the cell surface rather than secreted. This protein activates MMP2 protein, and this activity may be involved in tumor invasion.
      Gene Symbol
      • MMP14
      • MTMMP1
      • MMP-14
      • MT1MMP
      • MT1-MMP
      • MMP-X1
      • EC 3.4.24.80
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P50281 # Seems to specifically activate progelatinase A. May thus trigger invasion by tumor cells by activating progelatinase A on the tumor cell surface.
      COFACTOR: Binds 1 zinc ion per subunit (By similarity). & Calcium (By similarity).
      SIZE: 582 amino acids; 65884 Da
      SUBCELLULAR LOCATION: Membrane; Single-pass type I membrane protein (Potential). Melanosome. Note=Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
      TISSUE SPECIFICITY: In stromal cells of colon, breast, and head and neck.
      DOMAIN: SwissProt: P50281 The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.
      SIMILARITY: Belongs to the peptidase M10A family. & Contains 4 hemopexin-like domains.
      Molecular Weight60 kDa
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage Conditions1 year at 2-8°C from date of shipment
      Packaging Information
      Material Size100 µg
      Global Trade Item Number
      Catalogue Number GTIN
      MAB1767 04053252317798