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MABC667 Anti-GPC3 Antibody, clone 9C2

Detect Glypican using this mouse monoclonal antibody, Anti-GPC3 Antibody, clone 9C2 validated for use in western blotting, Immunofluorescence, IHC & Flow Cytometry.

Anti-GPC3 Antibody, clone 9C2 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Apoptosis & Cancer Research Sub-Category: Apoptosis - Additional Gene Symbol: GPC3, OCI5 UniProt Number: P51654
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MABC667
100 µL  
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      Overview

      Replacement Information

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      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, MWB, IF, IHC, FCMAscitesMonoclonal Antibody
      Description
      Catalogue NumberMABC667
      DescriptionAnti-GPC3 Antibody, clone 9C2
      Alternate Names
      • SGB
      • DGSX
      • MXR7
      • SDYS
      • SGBS
      • OCI-5
      • SGBS1
      • GTR2-2
      • Glypican-3
      • Intestinal protein OCI-5
      Background InformationGlypican-3 or GTR2-2 is the protein product of the gene GPC3. It is a cell surface heparin sulfate containing glycoprotein that plays an important role in the modulation of growth and development of mesodermal tissues and organs, as well as various cancers. Among its many known functions, it is a regulator of the dipeptidyl peptidase DPP4 (CD26). DPP4 plays an important role in glucose metabolism via its action on incretin and GLP-1 hormones. DPP4 also is a cancer marker and thought to function as a cancer suppressor. Thus acting as a regulator of DPP4, Glypican-3 (GPC3) participates in everything from glucose and blood sugar regulation to tumor growth. Glypican-3 (GPC3) is most highly expressed in the lung, liver and kidney, and typically a secreted form, secreted glypican-3, is found in the extracellular space generated as a cleavage product by the action of a convertase. Defects in GPC3 cause Simpson-Golabi-Behmel syndrome type 1 (SGBS1) or Simpson dysmorphia syndrome (SDYS). This is a condition characterized by pre-and postnatal overgrowth (gigantism) with visceral and skeletal anomalies. EMD-Millipore’s anti-GPC3 mouse monoclonal antibody, clone 9C2, has been tested in western blot against recombinant human protein, human HepG2, HEK293, Jurkat, and SK-N-SH cell line lysates, as well as rat PC-12 and mouse liver cell lysates. In addition, the clone as been successfully used in paraffin-embedded immunohistochemistry on human liver and breast cancer tissues and in immunofluorescent immunocytochemistry on human HeLa cells in culture. Finally, the clone has been tested successfully on human cells by flow cytometry and against the recombinant antigen by ELISA.
      References
      Product Information
      FormatAscites
      Control
      • Mouse liver, HepG2, HEK293, Jurkat, SK-N-SH, PC-12, and F9 cell lysates
      PresentationPurified mouse monoclonal in PBS with 0.05% sodium azide
      Quality LevelMQ100
      Applications
      ApplicationDetect Glypican using this mouse monoclonal antibody, Anti-GPC3 Antibody, clone 9C2 validated for use in western blotting, Immunofluorescence, IHC & Flow Cytometry.
      Key Applications
      • Western Blotting
      • Immunofluorescence
      • Immunohistochemistry
      • Flow Cytometry
      Application NotesImmunohistochemistry Analysis: A 1:100 dilution from a representative lot detected GPC3 in human liver and kidney tissues.

      Immunofluorescence Analysis: A 1:200-1,000 dilution from a representative lot detected GPC3 in HeLa cells.

      Flow Cytometry Analysis: A 1:200-400 dilution from a representative lot detected GPC3 in Jurkat cells.

      Optimal working dilutions must be determined by end user.
      Biological Information
      ImmunogenPurified recombinant fragment of human GPC3 expressed in E. Coli.
      Clone9C2
      HostMouse
      IsotypeIgG1
      Species Reactivity
      • Human
      • Mouse
      Antibody TypeMonoclonal Antibody
      Gene Symbol
      • GPC3
      • OCI5
      Purification MethodProtein G Purified
      UniProt Number
      Molecular Weight~66 kDa observed. Uncharacterized bands may appear in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in mouse liver, HepG2, HEK293, Jurkat, SK-N-SH, PC-12, and F9 cell lysates.

      Western Blotting Analysis: A 1:500-2,000 dilution of this antibody detected GPC3 in mouse liver, HepG2, HEK293, Jurkat, SK-N-SH, PC-12, and F9 cell lysates.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      MABC667 04053252918056

      Documentation

      Anti-GPC3 Antibody, clone 9C2 SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-GPC3 Antibody, clone 9C2 Certificates of Analysis

      TitleLot Number
      Anti-GPC3, clone 9C2 - VP1401035 VP1401035
      Anti-GPC3, clone 9C2 - VP1404281 VP1404281
      Anti-GPC3, clone 9C2 - QVP1305307 QVP1305307
      Anti-GPC3, clone 9C2 - VP1905161 VP1905161
      Anti-GPC3, clone 9C2 - VP1909200 VP1909200
      Anti-GPC3, clone 9C2 - VP2112011 VP2112011
      Anti-GPC3, clone 9C2 - VP2202171 VP2202171
      Anti-GPC3, clone 9C2 - VP2205192 VP2205192
      Anti-GPC3, clone 9C2 - VP2210043 VP2210043
      Anti-GPC3, clone 9C2 - VP2303020 VP2303020
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