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MAB3582 Anti-Fascin Antibody, clone 55K2

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MAB3582
100 µg  
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      Overview

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, Ma, Ech, Gr, SuWB, ICC, IH(P)MPurifiedMonoclonal Antibody
      Description
      Catalogue NumberMAB3582
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionAnti-Fascin Antibody, clone 55K2
      Product Information
      FormatPurified
      Control
      • POSITIVE CONTROL:

        Hela cells, neuronal tissues, antigen presenting dendritic cells.
      PresentationLiquid in 0.02M Phosphate buffer with 0.25M NaCl and 0.1% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationThis Anti-Fascin Antibody, clone 55K2 is validated for use in WB, IC, IH(P) for the detection of Fascin.
      Key Applications
      • Western Blotting
      • Immunocytochemistry
      • Immunohistochemistry (Paraffin)
      Applications Not Recommended
      • Immunoprecipitation
      Application NotesWestern blot. Recognizes a protein of 55 kDa. Suggested working dilution is 1:100-1:1,000. Suggested blocking buffer is 3% BSA in PBS or 3% non fat milk in PBS.

      Immunohistochemistry on formalin fixed, paraffin embedded tissue sections. Suggested working dilution is 1:100-1:1,000.

      Immunocytochemistry on cold methanol fixed cultured cells. Suggested working dilution is 1:100-1:1,000.

      Optimal working dilutions must be determined by the end user.
      Biological Information
      ImmunogenHuman fascin
      Clone55K2
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostMouse
      SpecificityFascin. The antibody is strongly positive when antigen-presenting dendritic cells are matured. MAB3582 is a sensitive marker for Reed-Sternberg Cells of Hodgkin's Disease. The protein is an actin bundling protein specifically localized in filopodia and growth cones.
      IsotypeIgG1
      Species Reactivity
      • Human
      • Mammals
      • Echinoderms
      • Gerbil
      • Sea Urchin
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • FSCN1
      • FAN1
      • HSN
      • FLJ38511
      • SNL
      • Fascin
      • p55
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: Q16658 # Organizes filamentous actin into bundles with a minimum of 4.1:1 actin/fascin ratio. Probably involved in the assembly of actin filament bundles present in microspikes, membrane ruffles, and stress fibers.
      SIZE: 493 amino acids; 54530 Da
      SUBUNIT: Associates with beta-catenin.
      TISSUE SPECIFICITY: Ubiquitous.
      PTM: Phosphorylation on Ser-39 inhibits the actin-binding ability of fascin.
      DISEASE: SwissProt: Q16658 # Marks and mediates breast cancer metastasis to the lungs. FSCN1 is not functionally validated but achieves the highest statistical significance (P less than 0.000001). Those subjects expressing the lung metastasis signature have a significantly poorer lung metastasis-free survival, but not bone metastasis-free survival, compared to subjects without the signature.
      SIMILARITY: SwissProt: Q16658 ## Belongs to the fascin family.
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsMaintain at 2-8°C in undiluted aliquots up to 6 months after date of receipt.
      Packaging Information
      Material Size100 µg
      Global Trade Item Number
      Catalogue Number GTIN
      MAB3582 04053252317866