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MABN2484-25UL Anti-ErbB4 Antibody, clone mAB10

Anti-ErbB4, clone mAB10, Cat. No. MABN2484, is a highly specific rabbit monoclonal antibody that targets Receptor tyrosine-protein kinase ErbB-4 and has been tested for use in ELISA, Immunohistochemistry (Paraffin), Immunoprecipitation, and Western Blotting.

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MABN2484-25UL
25 µL  
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      Overview

      Replacement Information
      Description
      Catalogue NumberMABN2484-25UL
      DescriptionAnti-ErbB4 Antibody, clone mAB10
      Alternate Names
      • Receptor tyrosine-protein kinase erbB-4
      • Proto-oncogene-like protein c-ErbB-4
      Background InformationReceptor tyrosine-protein kinase erbB-4 (UniProt: Q61527; also known as EC: 2.7.10.1, Proto-oncogene-like protein c-ErbB-4) is encoded by the Erbb4 (also known as Mer4) gene (Gene ID: 13869) in murine species. ErbB-4 is a single-pass type I membrane protein of the EGF receptor family that plays an essential role as cell surface receptor for neuregulins (NRG) and EGF family members. ErbB-4 is monomeric in the absence of bound ligand and it homo- or heterodimerizes with another ERBB family member upon ligand binding. ErbB-4 is autophosphorylated on tyrosine residues in response to ligand binding that triggers receptor dimerization and autophosphorylation at specific tyrosine residues, which then serve as binding sites for scaffold proteins and effectors. ErbB-4 is reported to regulate the development of the heart, the central nervous system, and the mammary gland. It is shown to be involved in gene transcription, cell proliferation, differentiation, migration, and apoptosis. It is expressed in the developing and adult cerebral cortex, and represents the major NRG receptor in central neurons and regulates a diverse array of neuronal processes, including migration, differentiation, neurotransmission, and synaptic plasticity. NRG-1 and ErbB-4 are essentials of a signaling pathway that is critical for the proper development of inhibitory cortical and hippocampal circuits. ErbB-4 expression is reported to be restricted to GABAergic neurons in the cerebral cortex where it plays a role in regulating GABAergic synaptic development and interneuron migration. ErbB-4 is synthesized with a signal peptide (aa 1-25), which is cleaved off in the mature form. The mature form has an extracellular domain (aa 26-652), a short transmembrane domain (aa 653-673), and a long cytoplasmic tail (aa 674-1308). Its protein kinase domain is localized in the cytoplasmic tail (aa 718-985). Three isoforms of ErbB-4 have been described that are produced by alternative splicing.
      References
      Product Information
      FormatPurified
      PresentationPurified rabbit monoclonal antibody IgG in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Applications
      ApplicationAnti-ErbB4, clone mAB10, Cat. No. MABN2484, is a highly specific rabbit monoclonal antibody that targets Receptor tyrosine-protein kinase ErbB-4 and has been tested for use in ELISA, Immunohistochemistry (Paraffin), Immunoprecipitation, and Western Blotting.
      Key Applications
      • ELISA
      • Immunohistochemistry (Paraffin)
      • Immunoprecipitation
      • Western Blotting
      Application NotesImmunohistochemistry (Paraffin) Analysis: A 1:250 dilution from a representative lot detected ErbB4 in mouse embryo tissue sections.

      Western Blotting Analysis: A representative lot detected ErbB4 in Western Blotting applications (Vullhorst, D., et. al. (2009). J Neurosci. 29(39):12255-64).

      ELISA Analysis: A representative lot detected ErbB4 in ELISA applications (Vullhorst, D., et. al. (2009). J Neurosci. 29(39):12255-64).

      Immunohistochemistry Analysis: A representative lot detected ErbB4 in Immunohistochemistry applications (Vullhorst, D., et. al. (2009). J Neurosci. 29(39):12255-64).

      Immunoprecipitation Analysis: A representative lot immunoprecipitated ErbB4 in Immunoprecipitation applications (Vullhorst, D., et. al. (2009). J Neurosci. 29(39):12255-64).
      Biological Information
      ImmunogenGST-tagged recombinant protein corresponding to 204 amino acids from the C-terminal region of murine Receptor tyrosine-protein kinase erbB-4.
      Clonemab10
      ConcentrationPlease refer to lot specific datasheet.
      HostRabbit
      SpecificityClone mAB10 is a rabbit monoclonal antibody that detects mouse Receptor tyrosine-protein kinase erbB-4. It targets an epitope with in the C-terminal region.
      IsotypeIgG
      Species Reactivity
      • Mouse
      Species Reactivity NoteMouse.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • Erbb4
      • Mer4
      Purification MethodProtein G purified
      UniProt Number
      Molecular Weight146.85 kDa calculated.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Immunohistochemistry (Paraffin) in mouse cerebral cortex tissue sections.

      Immunohistochemistry (Paraffin) Analysis: A 1:250 dilution of this antibody detected ErbB4 in mouse cerebral cortex tissue sections.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size25 µL
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      MABN2484-25UL 04054839613715

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