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MAB4444 Anti-Epithelial Cell Adhesion Molecule (EpCAM) Antibody, clone 8

This Anti-Epithelial Cell Adhesion Molecule (EpCAM) Antibody, clone 8 is validated for use in FC, IC, WB for the detection of Epithelial Cell Adhesion Molecule (EpCAM).

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Stem Cell Research Research Sub-Category: Pluripotent & Early Differentiation, Adhesion (CAMs) Gene Symbol: EPCAM, 17-1A, 323/A3, CD326, CO-17A, CO17-1A, EGP, EGP-2, EGP34, EGP40, ESA, Ep-CAM, GA733-2, HEA125, KS1/4, KSA, Ly74, M1S2, M4S1, MH99, MIC18, MK-1, MOC31, TACST-1, TACSTD1, TROP1, hEGP-2 UniProt Number: P16422
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MAB4444
100 µg  
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      Overview

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      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HFC, ICC, WBMPurifiedMonoclonal Antibody
      Description
      Catalogue NumberMAB4444
      DescriptionAnti-Epithelial Cell Adhesion Molecule (EpCAM) Antibody, clone 8
      Alternate Names
      • Adenocarcinoma-associated antigen
      • CD326 antigen
      • Cell surface glycoprotein Trop-1
      • Epithelial cell surface antigen
      • Epithelial glycoprotein
      • KS 1/4 antigen
      • Major gastrointestinal tumor-associated protein GA733-2
      • antigen identified by monoclonal antibody AUA1
      • carcinoma-associated antigen GA733-2
      • epithelial cell adhesion molecule
      • human epithelial glycoprotein-2
      • membrane component, chromosome 4, surface marker (35kD glycoprotein)
      • tumor-associated calcium signal transducer 1
      Background InformationEpithelial Call Adhesion Molecule (EpCAM, CD326) is a transmembrane glycoprotein of approximately 40 kDa. EpCAM was initially identified as a tumor associated marker having high expression in quickly proliferating epithelial tumors. As such, EpCAM has been of significant interest as a means for tumor diagnosis and therapy. EpCAM is also expressed in normal epithelial cells, but in comparatively lower levels. Preliminary studies limited EpCAM’s function to cell-cell adhesion; however more recent studies indicate it to be involved in diverse processes that include cell migration, cell signaling, differentiation and proliferation.
      References
      Product Information
      FormatPurified
      Control
      • Human embryonic stem cells
      PresentationPurified mouse monoclonal IgG2aκ in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationThis Anti-Epithelial Cell Adhesion Molecule (EpCAM) Antibody, clone 8 is validated for use in FC, IC, WB for the detection of Epithelial Cell Adhesion Molecule (EpCAM).
      Key Applications
      • Flow Cytometry
      • Immunocytochemistry
      • Western Blotting
      Application NotesFlow Cytometry Analysis: 0.5 µg from a previous lot detected Epithelial cell adhesion molecule in human embryonic stem cells.

      Western Blot Analysis: A previous lot was used by an independent laboratory in WB. (Ng, V., et al., 2010)
      Biological Information
      ImmunogenHES-3 Cells (Choo, et al. (2008) Stem Cells. Jun;26(6):1454-63)
      EpitopeUnknown
      Clonemab 8
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostMouse
      SpecificityThis antibody recognizes epithelial cell adhesion molecule (EpCAM), specifically on undifferentiated human embryonic stem cells.
      IsotypeIgG2aκ
      Species Reactivity
      • Human
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThis gene encodes a carcinoma-associated antigen and is a member of a family that includes at least two type I membrane proteins. This antigen is expressed on most normal epithelial cells and gastrointestinal carcinomas and functions as a homotypic calcium-independent cell adhesion molecule. The antigen is being used as a target for immunotherapy treatment of human carcinomas. Mutations in this gene result in congenital tufting enteropathy. [provided by RefSeq].
      Gene Symbol
      • EPCAM
      • 17-1A
      • 323/A3
      • CD326
      • CO-17A
      • CO17-1A
      • EGP
      • EGP-2
      • EGP34
      • EGP40
      • ESA
      • Ep-CAM
      • GA733-2
      • HEA125
      • KS1/4
      • KSA
      • Ly74
      • M1S2
      • M4S1
      • MH99
      • MIC18
      • MK-1
      • MOC31
      • TACST-1
      • TACSTD1
      • TROP1
      • hEGP-2
      Purification MethodProtein G Purified
      UniProt Number
      UniProt SummaryFUNCTION: May act as a physical homophilic interaction molecule between intestinal epithelial cells (IECs) and intraepithelial lymphocytes (IELs) at the mucosal epithelium for providing immunological barrier as a first line of defense against mucosal infection.

      SUBUNIT STRUCTURE: Monomer.

      SUBCELLULAR LOCATION: Membrane; Single-pass type I membrane protein.

      TISSUE SPECIFICTY: This protein is expressed in almost all epithelial cell membranes but not on mesodermal or neural cell membranes. Found on the surface of adenocarcinomas.

      SEQUENCE SIMILARITIES: Belongs to the EPCAM family.

      Contains 1 thyroglobulin type-1 domain.
      Molecular Weight~ 40 kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Immunocytochemistry in human embryonic stem cells.

      Immunocytochemistry Analysis: 1-10 µg/mL from a previous lot detected epithelial cell adhesion molecule (EpCAM) in human embryonic stem cells.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      MAB4444 04053252617447

      Documentation

      Anti-Epithelial Cell Adhesion Molecule (EpCAM) Antibody, clone 8 SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-Epithelial Cell Adhesion Molecule (EpCAM) Antibody, clone 8 Certificates of Analysis

      TitleLot Number
      Anti-Epithelial Cell Adhesion Molecule (EpCAM), clone mAb 8 (KC) Monoclonal Antibody 2957938
      Anti-Epithelial Cell Adhesion Molecule (EpCAM), clone mAb 8 - 2145979 2145979
      Anti-Epithelial Cell Adhesion Molecule (EpCAM), clone mAb 8 - 2397157 2397157
      Anti-Epithelial Cell Adhesion Molecule (EpCAM), clone mAb 8 - 2017301 2017301
      Anti-Epithelial Cell Adhesion Molecule (EpCAM), clone mAb 8 - 2088984 2088984
      Anti-Epithelial Cell Adhesion Molecule (EpCAM), clone mAb 8 - 2291714 2291714
      Anti-Epithelial Cell Adhesion Molecule (EpCAM), clone mAb 8 - 2561012 2561012
      Anti-Epithelial Cell Adhesion Molecule (EpCAM), clone mAb 8 - 3204853 3204853
      Anti-Epithelial Cell Adhesion Molecule (EpCAM), clone mAb 8 - 3698151 3698151
      Anti-Epithelial Cell Adhesion Molecule (EpCAM), clone mAb 8 - 3833724 3833724
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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies