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05-101 Anti-EGFR Antibody, neutralizing, clone LA1

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05-101
250 µg  
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      Overview

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HICC, IHC, IP, NEUT, WBMPurifiedMonoclonal Antibody
      Description
      Catalogue Number05-101
      Replaces04-337; 04-338
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionAnti-EGFR Antibody, neutralizing, clone LA1
      OverviewReacts with external domain of EGF receptor on all human cells. Competes with EGF and TGF-α for binding on human cells.
      Background InformationEpidermal growth factor receptor (UniProt: P00533; also known as EC:2.7.10.1, Proto-oncogene c-ErbB-1, Receptor tyrosine-protein kinase erbB-1) is encoded by the EGFR (also known as ERBB, ERBB1, HER1) gene (Gene ID: 1956) in human. EGFR is a single-pass type I membrane glycoprotein that is ubiquitously expressed. It is synthesized with a signal peptide (1-24), which is subsequently cleaved off to produce the mature form that contains an extracellular domain (aa 25-645), a transmembrane domain (aa 646-668), and a long cytoplasmic domain (669-1210). It possesses receptor tyrosine kinase activity and upon binding of ligands activates several signaling cascades. Ligand binding is known to trigger receptor homo- and/or heterodimerization, which stimulates its intrinsic intracellular protein-tyrosine kinase activity. As a result, autophosphorylation of tyrosine residues in the C-terminal domain of EGFR occurs, which results in activation of several downstream signaling cascades, including the Ras-RAF-MEK-ERK, PI3 kinase-Akt, PLCg-PKC, and STAT. EGFR phosphorylation at serine 695 is reported to be only partial and occurs only if threonine 693 is phosphorylated. EGFR also undergoes mono and polyubiquitination upon EGF stimulation. However, this ubiquitination does not affect tyrosine kinase activity of the receptor or its signaling capacity. EGFR is commonly overexpressed and is mutated in many human malignancies and is often associated with aggressive phenotypes.
      Product Information
      FormatPurified
      Control
      • Positive Antigen Control: Catalog #12-301, non-stimulated A431 cell lysate. Add 2.5µL of 2-mercaptoethanol/100µL of lysate and boil for 5 minutes to reduce the preparation. Load 20µg of reduced lysate per lane for mingels.
      PresentationLyophilized protein G Purified mouse immunoglobulin. Lyophilized from 0.1M Tris-glycine, pH 7.4, 0.15M NaCl containing no preservatives.
      Quality LevelMQ100
      Applications
      ApplicationDetect EGFR using this Anti-EGFR Antibody, neutralizing, clone LA1 validated for use in Immuncytochemistry, Immunohistochemistry, Immunoprecipitation, Neutralization, and Western Blotting.
      Key Applications
      • Immunocytochemistry
      • Immunohistochemistry
      • Immunoprecipitation
      • Neutralizing
      • Western Blotting
      Application NotesImmunocytochemistry (IC) Analysis: 2µg/mL of a representative lot detected EGFR in A431 cells.
      Immunohistochemistry (Paraffin) (IHC) Analysis: A 1:50 dilution of this antibody detected EGFR in Human placenta tissue sections.
      Immunoprecipitation Analysis: A representative lot of this antibody immunoprecipitated EGFR in Huh-7.5 cells. (Diao, J., et al. (2012). J. Virol. 86(20); 10935-10949).
      Western Blotting Analysis: 1 mg/mL of a representative lot detected EGFR in A431 cell lysate.
      Biological Information
      ImmunogenHuman A431 membrane proteins.
      CloneLA1
      HostMouse
      SpecificityRecognizes both phosphorylated and non-phosphorylated human EGF receptors.
      IsotypeIgG1
      Species Reactivity
      • Human
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • EGFR
      • ERBB1
      • ERBB
      • mENA
      Purification MethodProtein G Purified
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P00533 # Isoform 2/truncated isoform may act as an antagonist.
      SIZE: 1210 amino acids; 134277 Da
      SUBUNIT: Binds RIPK1. CBL interacts with the autophosphorylated C- terminal tail of the EGF receptor. Part of a complex with ERBB2 and either PIK3C2A or PIK3C2B. The autophosphorylated form interacts with PIK3C2B, maybe indirectly. Interacts with PELP1.
      SUBCELLULAR LOCATION: Cell membrane; Single-pass type I membrane protein. & Isoform 2: Secreted.
      TISSUE SPECIFICITY: Expressed in placenta. Isoform 2 is also expressed in ovarian cancers.
      PTM: Phosphorylation of Ser-695 is partial and occurs only if Thr- 693 is phosphorylated. & Monoubiquitinated and polyubiquitinated upon EGF stimulation; which does not affect tyrosine kinase activity or signaling capacity but may play a role in lysosomal targeting. Polyubiquitin linkage is mainly through 'Lys-63', but linkage through 'Lys-48', 'Lys-11' and 'Lys-29' also occur.DISEASE:SwissProt: P00533 # Defects in EGFR are associated with lung cancer [MIM:211980].
      SIMILARITY: SwissProt: P00533 ## Belongs to the protein kinase superfamily. Tyr protein kinase family. EGF receptor subfamily. & Contains 1 protein kinase domain.
      MISCELLANEOUS: Binding of EGF to the receptor leads to dimerization, internalization of the EGF-receptor complex, induction of the tyrosine kinase activity, stimulation of cell DNA synthesis, and cell proliferation.
      Molecular Weight180 kDa
      Product Usage Statements
      Quality Assuranceroutinely evaluated on RIPA lysates from non-stimulated human A431 cells
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsLyophilized: 2 years at -20°C; Rehydrated: 1 month at 4°C or 6 months at -20°C
      Packaging Information
      Material Size250 µg
      Global Trade Item Number
      Catalogue Number GTIN
      05-101 04053252470356