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ABS1645 Anti-Chromatin-Modifying Protein 6

Anti-Chromatin-Modifying Protein 6, Cat. No. ABS1645, is a rabbit polyconal antibody that is used to detect Chromatin-Modifying Protein 6 and has been tested for use in Immunofluorescence and Western Blotting.

Anti-Chromatin-Modifying Protein 6 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Signaling Gene Symbol: CHMP6, VPS20 UniProt Number: Q96FZ7
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ABS1645
100 μg  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, MkWB, IFRbPurifiedPolyclonal Antibody
      Description
      Catalogue NumberABS1645
      DescriptionAnti-Chromatin-Modifying Protein 6
      Alternate Names
      • Chromatin-modifying protein 6
      • Charged multivesicular body protein 6
      • Vacuolar protein sorting-associated protein 20
      • Vps20
      • hVps20
      Background InformationCharged multivesicular body protein 6 (UniProt: Q96FZ7; also known as Chromatin-modifying protein 6, Vacuolar protein sorting-associated protein 20, Vps20, hVps20, CHMP6) is encoded by the CHMP6 (also known as VPS20) gene (Gene ID: 79643) in human. CHMP6 is a human orthologue of yeast Vps (vacuolar protein sorting) 20, a component of ESCRT (endosomal sorting complex required for transport)-III. Various CHMP6 orthologues in organisms ranging from yeast to humans contain the N-myristoylation consensus sequence at each N-terminus. CHMP6 is an ubiquitously expressed protein that has an acidic C-terminus and the basic N-terminus that render the protein in a closed, soluble and inactive conformation through an auto-inhibitory intramolecular interaction. The open and active conformation, which enables membrane binding and oligomerization, is achieved by interaction with other cellular binding partners, probably including other ESCRT components. CHMP6 is probable core component of the endosomal sorting required for transport complex III (ESCRT-III), which is involved in multivesicular bodies (MVBs) formation and sorting of endosomal cargo proteins into MVBs. MVBs contain intraluminal vesicles that are generated by invagination and scission from the limiting membrane of the endosome and mostly are delivered to lysosomes enabling degradation of membrane proteins.
      References
      Product Information
      FormatPurified
      PresentationPurified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationAnti-Chromatin-Modifying Protein 6, Cat. No. ABS1645, is a rabbit polyconal antibody that is used to detect Chromatin-Modifying Protein 6 and has been tested for use in Immunofluorescence and Western Blotting.
      Key Applications
      • Western Blotting
      • Immunofluorescence
      Application NotesImmunofluorescence Analysis: A 1:1,000 dilution from a representative lot detected Chromatin-Modifying Protein 6 in Cos-7 cells (Langelier, C., et. al. (2006). J Virol. 80(19):9465-80).

      Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected Chromatin-Modifying Protein 6 in HEK293 cell lysate (Langelier, C., et. al. (2006). J Virol. 80(19):9465-80).
      Biological Information
      ImmunogenGST-tagged full length recombinant human Chromatin-modifying protein 6.
      Epitopeunknown
      ConcentrationPlease refer to lot specific datasheet.
      HostRabbit
      SpecificityThis polyclonal antibody detects chromatin-modifying protein 6 (CHMP6) in human and monkey cells.
      IsotypeIgG
      Species Reactivity
      • Human
      • Monkey
      Species Reactivity NoteHuman, Monkey.
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • CHMP6
      • VPS20
      Purification MethodAffinity Purified
      UniProt Number
      Molecular Weight~32 kDa observed; 23.48 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in HEK293 cell lysate.

      Western Blotting Analysis: A 1:2,000 dilution of this antibody detected Chromatin-Modifying Protein 6 in 10 µg of HEK293 cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      ABS1645 04054839201370