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MABT217 Anti-Calreticulin Antibody, clone 16B11.1

This Anti-Calreticulin Antibody, clone 16B11.1 is validated for use in Western Blotting, IHC, Flow Cytometry for the detection of Calreticulin.

Anti-Calreticulin Antibody, clone 16B11.1 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Cell Structure Research Sub-Category: Adhesion (CAMs) Gene Symbol: CALR, CRTC UniProt Number: P27797
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MABT217
100 µL  
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      Overview

      Replacement Information

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      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HWB, IHC, FCMPurifiedMonoclonal Antibody
      Description
      Catalogue NumberMABT217
      ReplacesMABT145
      DescriptionAnti-Calreticulin Antibody, clone 16B11.1
      Alternate Names
      • Calreticulin
      • CRP55
      • Calregulin
      • Endoplasmic reticulum resident protein 60
      • ERp60
      • HACBP
      • grp60
      Background InformationCalreticulin is a molecular Ca+ binding chaperone promoting folding, oligomeric assembly, and quality control in ER. Calreticulin is a highly versatile lectin-like chaperone, and it participates during the synthesis of a variety of molecules, including ion channels, surface receptors, integrins and transporters. Calreticulin indeed provides up to 45% of the Ca2+-buffering capacity for a pool of the IP3-sensitive Ca2+ inside the ER. Calreticulin also functions in cell adhesion, integrin-dependent Ca2+ signaling and steroid-sensitive gene expression.
      References
      Product Information
      FormatPurified
      Control
      • HeLa cell lysate
      PresentationPurified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationThis Anti-Calreticulin Antibody, clone 16B11.1 is validated for use in Western Blotting, IHC, Flow Cytometry for the detection of Calreticulin.
      Key Applications
      • Western Blotting
      • Immunohistochemistry
      • Flow Cytometry
      Application NotesImmunohistochemistry Analysis: A 1:300-1,000 dilution from a representative lot detected Calreticulin in human colorectal cancer and human tonsil tissues.

      Flow Cyometry Analysis: A 1:500 dilution from a representative lot detected Calreticulin in non serum starved HeLa cells.
      Biological Information
      ImmunogenGST-tagged recombinant protein corresponding to human Calreticulin.
      Clone16B11.1
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostMouse
      IsotypeIgG1κ
      Species Reactivity
      • Human
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • CALR
      • CRTC
      Purification MethodProtein G Purified
      UniProt Number
      Molecular Weight~48 kDa observed
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blot in HeLa cell lysate.

      Western Blot Analysis: A 1:1,000 dilution of this antibody detected Calreticulin on 10 µg of HeLa cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      MABT217 04053252646324

      Documentation

      Anti-Calreticulin Antibody, clone 16B11.1 SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-Calreticulin Antibody, clone 16B11.1 Certificates of Analysis

      TitleLot Number
      Anti-Calreticulin, clone 16B11.1 2487734
      Anti-Calreticulin, clone 16B11.1 - 2452664 2452664
      Anti-Calreticulin, clone 16B11.1 - 2464644 2464644
      Anti-Calreticulin, clone 16B11.1 - 3234490 3234490
      Anti-Calreticulin, clone 16B11.1 - 4101908 4101908
      Anti-Calreticulin, clone 16B11.1 - Q2015737 Q2015737
      Anti-Calreticulin, clone 16B11.1 -2683318 2683318
      Anti-Calreticulin, clone 16B11.1 -2736482 2736482
      Anti-Calreticulin, clone 16B11.1 -2740941 2740941
      Anti-Calreticulin, clone 16B11.1 -2761621 2761621
      Show more

      Brochure

      Title
      New Products: Volume 3, 2012

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies