Our broad portfolio consists of multiplex panels that allow you to choose, within the panel, analytes that best meet your needs. On a separate tab you can choose the premixed cytokine format or a single plex kit.
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Choose fixed kits that allow you to explore entire pathways or processes. Or design your own kits by choosing single plex MAPmates™, following the provided guidelines.
The following MAPmates™ should not be plexed together:
-MAPmates™ that require a different assay buffer
-Phospho-specific and total MAPmate™ pairs, e.g. total GSK3β and GSK3β (Ser 9)
-PanTyr and site-specific MAPmates™, e.g. Phospho-EGF Receptor and phospho-STAT1 (Tyr701)
-More than 1 phospho-MAPmate™ for a single target (Akt, STAT3)
-GAPDH and β-Tubulin cannot be plexed with kits or MAPmates™ containing panTyr
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48-602MAG
Buffer Detection Kit for Magnetic Beads
1 Kit
Space Saver Option Customers purchasing multiple kits may choose to save storage space by eliminating the kit packaging and receiving their multiplex assay components in plastic bags for more compact storage.
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Anti-CD3e Antibody, clone UCHT1 is an antibody against CD3e for use in Flow Cytometry, Immunoprecipitation, ELISA, Immunocytochemistry, Immunohistochemistry, Affects Function.
More>>Anti-CD3e Antibody, clone UCHT1 is an antibody against CD3e for use in Flow Cytometry, Immunoprecipitation, ELISA, Immunocytochemistry, Immunohistochemistry, Affects Function. Less<<
Anti-CD3e Antibody, clone UCHT1 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
T-cell surface glycoprotein CD3 epsilon chain (UniProt P07766; also known as CD3-epsilon, CD3e, T-cell surface antigen T3/Leu-4 epsilon chain) is encoded by the CD3E (also known as T3E) gene (Gene ID 916) in human. The T cell receptor or TCR is a plasma membrane protein complex responsible for recognizing antigens bound to major histocompatibility complex (MHC) on antigen presenting cells. In 95% of T cells, the TCR is composed of a disulfide-linked alpha (α) and beta (β) dimer, while among 5% of T cells the TCR is composed of a gamma (γ) and a delta (δ) dimer. T cells are activated when their surface TCRs engage with antigen/MHC complex on antigen presenting cells. Each TCR alpha and beta chain contains a variable (V) and a constant (C) extracellular immunoglobulin-like domain, followed by a transmembrane region and a short cytoplasmic tail. The alpha and beta chains have very short cytoplasmic tail and rely on the CD3 (cluster of differentiation 3) co-receptor complex for transducing signaling further intracellularly. The CD3 co-receptor complex is composed of a CD3γ chain, a CD3δ chain, and two CD3ε chains. The CD3 complex, TCR dimer, and a ζ-chain together comprise the functional TCR complex. The CD3 epsilon chain is initially produced with a signal peptide (a.a. 1-22), the removal of which yields the mature chain (a.a. 23-207) with an Ig-like (a.a. 32-112) domain-containing extracellular region (a.a. 23-126), a transmembrane segment (a.a. 127-152), and a cytoplasmic tail (a.a. 153-207), which contains the immunoreceptor tyrosine-based activation motif or ITAM (a.a. 178-205) that is essential for mediating ZAP-70 interaction and transducing TCR signaling.
References
Product Information
Format
Purified
Presentation
Purified mouse monoclonal IgG1κ antibody in PBS without preservatives.
Anti-CD3e Antibody, clone UCHT1 is an antibody against CD3e for use in Flow Cytometry, Immunoprecipitation, ELISA, Immunocytochemistry, Immunohistochemistry, Affects Function.
Key Applications
Flow Cytometry
Immunoprecipitation
ELISA
Immunocytochemistry
Immunohistochemistry
Affects Function
Application Notes
Immunoprecipitation Analysis: Representative lots immunoprecipitated CD3 epsilon-associated TCR complex (Schamel, W.W., et al. (2005). J. Exp. Med. 202(4):493-503; Poggi, A., et al. (1996). Int. Immunol. 8(12):1947-1953). ELISA Analysis: A representative lot was used as the capture antibody for the detection of epsilon-associated TCR complex proteins by sandwich ELISA (Poggi, A., et al. (1996). Int. Immunol. 8(12):1947-1953). Immunocytochemistry Analysis: A representative lot detected the exogensously expressed human CD3 epsilon chain on the surface of murine T cells by flourescent immunocytochemistry (Clevers, H., et al. (1988). Eur. J. Immunol. 18(5):705-710). Immunocytochemistry Analysis: A representative lot was employed to determined the plasma membrane and cytoplasmic CD3 immunoreactivity in T cell populations and T-ALL blast cells by flourescent immunocytochemistry (Campana, D., et al. (1987). J. Immunol. 138(2):648-655). Immunohistochemistry Analysis: A representative lot detected CD3+ T cells (T3+) in the periglandular stromal aggregates in frozen dometrium sections from female donors undergone routine dilation and curettage procedures (Kamat, B.R., and Isaacson, P.G. (1987). Am. J. Pathol. 127(1):66-73). Affects Function: A representative lot induced surface IL-2R expression and proliferation of human peripheral blood T lymphocytes (Van Wauwe, J.P., et al. (1984). J. Immunol. 133(1):129-132). Flow Cytometry Analysis: A representative lot was employed for fluorescence activated cell sorting (FACS) of CD3+ human lymphocytes (Beverley, P.C., and Callard, R.E. (1981). Science. 11(4):329-334).
Biological Information
Immunogen
Human infant thymocytes and lymphocytes from a patient with Sézary disease.
Epitope
Extracellular domain.
Clone
UCHT1
Concentration
Please refer to lot specific datasheet.
Host
Mouse
Specificity
Clone UCHT1 reacts with the extracellular domain of human CD3 epsilon chain (Clevers, H., et al. (1988). Eur. J. Immunol. 18(5):705-710).
23.15 kDa (Proform) and 20.74 (mature form) calculated. ~27 kDa reported in UCHT1 immunoprecipitate (Poggi, A., et al. (1996). Int. Immunol. 8(12):1947-1953).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality Assurance
Evaluated by Flow Cytometry in human PBMC.
Flow Cytometry Analysis: 0.1 µg of this antibody detected CD3e in 1X10E6 human PBMC.
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions
Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
