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MABN1547 Anti-CASK Antibody, clone 2G5.1

This Anti-CASK Antibody, clone 2G5.1 is validated for use in Western Blotting, Immunohistochemistry (Paraffin) for the detection of CASK.

Anti-CASK Antibody, clone 2G5.1 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Neuroscience Research Sub-Category: Developmental Signaling Gene Symbol: CASK, LIN2 UniProt Number: O14936
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MABN1547
100 μg  
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      Overview

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      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      R, HWB, IH(P)MPurifiedMonoclonal Antibody
      Description
      Catalogue NumberMABN1547
      ReplacesMAB5230
      DescriptionAnti-CASK Antibody, clone 2G5.1
      Alternate Names
      • Peripheral plasma membrane protein CASK
      • hCASK
      • Calcium/calmodulin-dependent serine protein kinase
      • Protein lin-2 homolog
      • CASK
      Background InformationPeripheral plasma membrane protein CASK (EC 2.7.11.1; UniProt O14936; also known as Calcium/calmodulin-dependent serine protein kinase, Membrane-associated guanylate kinase, hCASK, Protein lin-2 homolog, Trinucleotide repeat containing 8) is encoded by the CASK (also known as CAGH39, CAMGUK, CMG, FGS4, LIN2, MICPCH, MRXSNA, TNRC8) gene (Gene ID 8573) in human. CASK is a membrane-associated guanylate kinase (MAGUK) family member involved in the regulation of cytoskeletal remodeling and cell migration. It consists of a Ca2+-calmodulin kinase, a PDZ, an SH3, and a guanylate kinase domain. CASK localizes at cell–cell adhesion sites, including synaptic junctions, epithelial junctions, and tight junctions, where it is reported to associate with other cell adhesion molecules, such as junctional adhesion molecule (JAM), cadherins, catenins, and syndecan-2. CASK and syndecan-2 expressions are upregulated in colorectal cancer and their upregulation significantly correlates with advanced tumor stage, lymphatic invasion, lymph node metastasis, vascular invasion, liver metastasis, and unresectable metastatic CRC.
      References
      Product Information
      FormatPurified
      PresentationPurified mouse monoclonal IgG2aκ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationThis Anti-CASK Antibody, clone 2G5.1 is validated for use in Western Blotting, Immunohistochemistry (Paraffin) for the detection of CASK.
      Key Applications
      • Western Blotting
      • Immunohistochemistry (Paraffin)
      Application NotesImmunohistochemistry Analysis: A 1:50 dilution from a representative lot detected CASK in human kidney cortex tissue.
      Biological Information
      ImmunogenGST-tagged recombinant protein corresponding to an internal fragment within the C-terminal half of human CASK.
      EpitopeInternal (C-terminal half)
      Clone2G5.1
      ConcentrationPlease refer to lot specific datasheet.
      HostMouse
      IsotypeIgG2aκ
      Species Reactivity
      • Rat
      • Human
      Species Reactivity NoteRat, Human. Predicted to react with Mouse based on 100% sequence homology.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • CASK
      • LIN2
      Purification MethodProtein G Purified
      UniProt Number
      Molecular Weight~115 kDa observed
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in rat brain tissue lysate.

      Western Blotting Analysis: 1.0 µg/mL of this antibody detected CASK in 10 µg of rat brain tissue lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      MABN1547 04055977182811

      Documentation

      Anti-CASK Antibody, clone 2G5.1 SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-CASK Antibody, clone 2G5.1 Certificates of Analysis

      TitleLot Number
      Anti-CASK, clone 2G5.1 - 3432453 3432453
      Anti-CASK, clone 2G5.1 - 4113022 4113022
      Anti-CASK, clone 2G5.1 -Q2589602 Q2589602
      Anti-CASK, clone 2G5.1 -Q2589617 Q2589617

      Technical Info

      Title
      Characterization of Estrogen Receptor α Phosphorylation Sites in Breast Cancer Tissue Using the SNAP i.d® 2.0 System
      White Paper: Further considerations of antibody validation and usage.