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MABN43 Anti-Apolipoprotein E4 (ApoE4) Antibody, clone 4E4

This Anti--Apolipoprotein E4 antibody is validated for use in IP, WB, IH for the detection of ApoE4.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Neuroscience Research Sub-Category: Developmental Neuroscience Gene Symbol: APOE UniProt Number: P02649
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MABN43
100 µL  
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      Overview

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      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HWB, IHC, IPMPurifiedMonoclonal Antibody
      Description
      Catalogue NumberMABN43
      DescriptionAnti-Apolipoprotein E4 (ApoE4) Antibody, clone 4E4
      Alternate Names
      • Apolipoprotein E
      • Apo-E
      • ApoE4
      • Apolipoprotein E4
      Background InformationApolipoprotein is a polymorphic plasma protein that exists as three isoforms: ApoE4, ApoE3, and ApoE2, which differ in the combination of cysteine and arginine residues located at position 122 and 158. The 35 kDa ApoE4 isoform contains arginine at both locations, and is produced in the liver and brain, although it is widely expressed in other tissues, such as the lung, spleen, and ovary. Like other isoforms, ApoE4 is a ligand for low density lipoprotein receptors (LDL) and plays a critical role in the transport and metabolism of lipids. In addition to its role in maintaining cardiovascular function, ApoE4 is also critically involved in neuronal plasticity, synaptogenesis, aging, and neurodegenerative disorders. Mice deficient in ApoE4 show age-dependent abnormalities in synaptic structure and impaired long term potentiation. Additionally, ApoE4 has a well-documented role in the pathology of Alzheimer’s disease.
      References
      Product Information
      FormatPurified
      Control
      • Recombinant Apolipoprotein E protein
      PresentationPurified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationThis Anti--Apolipoprotein E4 antibody is validated for use in IP, WB, IH for the detection of ApoE4.
      Key Applications
      • Western Blotting
      • Immunohistochemistry
      • Immunoprecipitation
      Application NotesImmunohistochemistry Analysis: A 1:2,000-4,000 dilution from a representative lot detected ApoE4 in human Alzheimer's diseased brain and human cerebellum tissues.

      Immunoprecipitation Analysis: A representative lot was used by an independent laboratory to detect ApoE4 in CHO cells secreting ApoE4. (Image courtesy of Dr. Egon Ogris, Medical University of Vienna)
      Biological Information
      ImmunogenKLH-conjugated linear peptide corresponding to human ApoE4.
      Clone4E4
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostMouse
      SpecificityThis antibody recognizes ApoE4 but not ApoE3 or ApoE2.
      IsotypeIgG1κ
      Species Reactivity
      • Human
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • APOE
      Purification MethodProtein G Purified
      UniProt Number
      Molecular Weight~35 kDa observed
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blot in recombinant ApoE4 protein.

      Western Blot Analysis: A 1:100,000 dilution detected ApoE4 in 10 µg of recombinant ApoE4 protein.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      MABN43 04053252695889

      Documentation

      Anti-Apolipoprotein E4 (ApoE4) Antibody, clone 4E4 SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-Apolipoprotein E4 (ApoE4) Antibody, clone 4E4 Certificates of Analysis

      TitleLot Number
      Anti-Apolipoprotein E4 (ApoE4), clone 4E4 - 2464648 2464648
      Anti-Apolipoprotein E4 (ApoE4), -2604838 2604838
      Anti-Apolipoprotein E4 (ApoE4), -2820249 2820249
      Anti-Apolipoprotein E4 (ApoE4), clone 4E4 2478024
      Anti-Apolipoprotein E4 (ApoE4), clone 4E4 - 3231009 3231009
      Anti-Apolipoprotein E4 (ApoE4), clone 4E4 - 3290432 3290432
      Anti-Apolipoprotein E4 (ApoE4), clone 4E4 - 3445791 3445791
      Anti-Apolipoprotein E4 (ApoE4), clone 4E4 - 3515609 3515609
      Anti-Apolipoprotein E4 (ApoE4), clone 4E4 - 3708265 3708265
      Anti-Apolipoprotein E4 (ApoE4), clone 4E4 - 3957440 3957440
      Show more

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies