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MABS2024-25UG Anti-Acetyl CoA Carboxylase 1 Antibody, clone 10H2.1

Anti-Acetyl CoA Carboxylase 1, clone 10H2.1, Cat. No. MABS2024, is a mouse monoclonal antibody that detects Acetyl-CoA carboxylase 1 and has been tested for use in Western Blotting and Immunohistochemistry (Paraffin).

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Signaling Gene Symbol: ACACA, ACAC, ACC1, ACCA UniProt Number: Q13085
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MABS2024-25UG
25 μg  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HWB, IH(P)MPurifiedMonoclonal Antibody
      Description
      Catalogue NumberMABS2024-25UG
      DescriptionAnti-Acetyl CoA Carboxylase 1 Antibody, clone 10H2.1
      Alternate Names
      • ACC1
      • ACC-alpha
      Background InformationAcetyl-CoA carboxylase 1 (UniProt: Q13085; also known as ACC1, ACC-alpha) is encoded by the ACACA (also known as ACAC, ACC1, ACCA) gene (Gene ID: 31) in human. It catalyzes the rate-limiting reaction in the biogenesis of long-chain fatty acids. ACC1 is a multi-subunit, biotin-dependent enzyme that catalyzes carboxylation of acetyl-CoA to produce malonyl-CoA through its two catalytic activities, biotin carboxylase (BC) and carboxyltransferase (CT). The activity of ACC can be controlled at the transcriptional level as well as by small molecule modulators and covalent modification. Two isoforms fof ACC have been reported, alpha and beta that are encoded by two different genes. ACC-alpha is highly enriched in lipogenic tissues. ACC1 levels are regulated under long term control at the transcriptional and translational levels and under short term regulation by the phosphorylation/dephosphorylation of targeted serine residues and by allosteric transformation by citrate or palmitoyl-CoA. ACC1 activity is inhibited upon phosphorylation, which occurs when glucagon or epinephrine binds to the receptors or when the energy status of the cell is low, leading to the activation of the AMP-activated protein kinase. The presence of fatty acids inhibits the activities of ACC1. ACC is considered as an important target in the design of new anti-obesity drugs.
      References
      Product Information
      FormatPurified
      PresentationPurified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Applications
      ApplicationAnti-Acetyl CoA Carboxylase 1, clone 10H2.1, Cat. No. MABS2024, is a mouse monoclonal antibody that detects Acetyl-CoA carboxylase 1 and has been tested for use in Western Blotting and Immunohistochemistry (Paraffin).
      Key Applications
      • Western Blotting
      • Immunohistochemistry (Paraffin)
      Application NotesImmunohistochemistry Analysis: A 1:50 dilution from a representative lot detected Acetyl CoA Carboxylase 1 in human liver and human kidney tissue sections.
      Biological Information
      ImmunogenGST-tagged recombinant fragment corresponding to 245 amino acids from the C-terminal region of human Acetyl-CoA carboxylase 1.
      Clone10H2.1
      ConcentrationPlease refer to lot specific datasheet.
      HostMouse
      SpecificityClone 10H2.1 detects human Acetyl-CoA carboxylase 1. It targets an epitope within 245 amino acids from the C-terminal end.
      IsotypeIgG1κ
      Species Reactivity
      • Human
      Species Reactivity NoteHuman.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • ACACA
      • ACAC
      • ACC1
      • ACCA
      Purification MethodProtein G purified
      UniProt Number
      Molecular Weight~265 kDa observed; 265.55 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in A431 cell lysate.

      Western Blotting Analysis: 0.5 µg/mL of this antibody detected Acetyl CoA Carboxylase 1 in A431 cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size25 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      MABS2024-25UG 04054839468933