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MABT388 Anti-ADAM 17 Antibody, clone 16C11.1 | MABT388

This mouse Monoclonal Anti-ADAM 17 Antibody, clone 16C11.1, Cat. No. MABT388 detects levels of ADAM17, and has been published and validated for use in Western Blotting, Immunohistochemistry (Paraffin)

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Research Category: Cell Structure Research Sub-Category: Adhesion (CAMs) Gene Symbol: ADAM17, CSVP, TACE UniProt Number: P78536
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MABT388
100 μg  
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      Overview

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      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HWB, IH(P)MPurifiedMonoclonal Antibody
      Description
      Catalogue NumberMABT388
      DescriptionAnti-ADAM 17 Antibody, clone 16C11.1 | MABT388
      Alternate Names
      • Disintegrin and metalloproteinase domain-containing protein 17
      • ADAM 17
      • Snake venom-like protease
      • TNF-alpha convertase
      • TNF-alpha-converting enzyme
      • CD156b
      Background InformationDisintegrin and metalloproteinase domain-containing protein 17 (EC 3.4.24.86; UniProt P78536; also known as ADAM 17, CD156b, Snake venom-like protease. TNF-alpha convertase, TNF-alpha-converting enzyme, Tumor necrosis factor alpha-converting enzyme) is encoded by the ADAM17 (also known as CD156B, CSVP, NISBD, NISBD1, TACE) gene (Gene ID 6868) in human. Originally described as a TNF–alpha converting enzyme, ADAM17 is known to process the cleavage of many transmembrane proteins, including membrane-anchored cytokines, cell adhesion molecules, receptors, ligands, and enzymes. ADAM17 was the first 'sheddase' identified. ADAM17 activity is regulated by multiple post-translational modifications. It has three phosphorylation sites on its C-terminal cytoplasmic domain (T735, S791, and (S819). ERK1/2-mediated T735 phosphorylation is reported to regulate ADAM17 maturation and surface expression. ADAM 17 is initially translated in its prepro-form and subsequent cleavages of the signal (aa 1-17) and propeptide (18-214) sequences produces the 610 aa mature ADAM 17 (aa 215-824).
      Product Information
      FormatPurified
      PresentationPurified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationThis mouse Monoclonal Anti-ADAM 17 Antibody, clone 16C11.1, Cat. No. MABT388 detects levels of ADAM17, and has been published and validated for use in Western Blotting, Immunohistochemistry (Paraffin).
      Key Applications
      • Western Blotting
      • Immunohistochemistry (Paraffin)
      Application NotesWestern Blotting Analysis: 5.0 µg/mL from a representative lot detected ADAM 17 in 10 µg of human acute myelogenous leukemia KG-1 cell lysate.
      Immunohistochemistry Analysis: A 1:50-250 dilution from a representative lot detected ADAM 17 in human testis and colorectal cancer tissues.
      Biological Information
      ImmunogenGST-tagged recombinant protein corresponding to human ADAM 17.
      Clone16C11.1
      ConcentrationPlease refer to lot specific datasheet.
      HostMouse
      SpecificityExpected to react with both spliced isoforms.
      IsotypeIgG1κ
      Species Reactivity
      • Human
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • ADAM17
      • CSVP
      • TACE
      Purification MethodProtein G Purified
      UniProt Number
      Molecular Weight~120 kDa observed. A lower band ~100 kDa is also observed when using a higher antibody concentration (5 µg/mL). Due to glycosylation, target band size appears larger than the calculated molecular weights (93.0 kDa/prepro-isoform A, 68.2 kDa/mature isoform A, 78.5kDa/prepro-isoform B, 63.7 kDa/mature isoform B). Uncharacterized band(s) may appear in some lysates.
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in human acute myelogenous leukemia KG-1 cell lysate.

      Western Blotting Analysis: 0.5 µg/mL of this antibody detected ADAM 17 in 10 µg of human acute myelogenous leukemia KG-1 cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 μg
      Global Trade Item Number
      Catalogue Number GTIN
      MABT388 04055977176506