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MABE251 Anti-5-hydroxymethylcytosine (5hmC) Antibody, clone HMC 31

Detect 5-hydroxymethylcytosine (5hmC) with Anti-5-hydroxymethylcytosine (5hmC) Antibody, clone HMC 31 (Mouse Monoclonal Antibody), that has been shown to work in DB, MeDIP, ELISA.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Epigenetics & Nuclear Function Research Sub-Category: Chromatin Biology
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MABE251
100 µg  
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      Overview

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      100% Performance Guaranteed

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      ADB, Methylated DNA Immunoprecipitation (MeDIP), ELISAMPurifiedMonoclonal Antibody
      Description
      Catalogue NumberMABE251
      DescriptionAnti-5-hydroxymethylcytosine (5hmC) Antibody, clone HMC 31
      Background Information5-Hydroxymethylcytosine is a DNA pyrimidine nitrogen base formed from the enzymatic conversion of 5-methylcytosine into 5-hydroxymethylcytosine by the TET family of iron-dependent oxygenases. Data suggests that every mammilian cell contains 5-hydroxymethylcytosine, but the levels vary depending on the cell type; data also suggests that levels of 5-hydroxymethylcytosine increases with age. The highest levels are found in neuronal cells of the central nervous system and certain mammalian tissues such as mouse Purkinje and granule neurons. Although the exact function has not been fully elucidated, studies suggest that 5-hydroxymethlcytosine may regulate gene expression or initiate DNA demethylation.
      Product Information
      FormatPurified
      Control
      • Unmodified and modified cytosine DNA.
      PresentationPurified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationDetect 5-hydroxymethylcytosine (5hmC) with Anti-5-hydroxymethylcytosine (5hmC) Antibody, clone HMC 31 (Mouse Monoclonal Antibody), that has been shown to work in DB, MeDIP, ELISA.
      Key Applications
      • Dot Blot
      • Methylated DNA Immunoprecipitation (MeDIP)
      • ELISA
      Application NotesELISA Analysis: A representative lot detected 5-hydroxymethylcytosine in ELISA.

      Methylated DNA Immunoprecipitation (MeDIP) Analysis: A representative lot immunoprecipitated 5-hydroxymethylcytosine in ChIP.
      Biological Information
      ImmunogenBSA-conjugated 5-hydroxymethylcytosine.
      Epitope5-hydroxymethylcytosine
      CloneHMC 31
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostMouse
      SpecificityThis antibody recognizes the 5-hydroxymethylcytosine of 5-Hydroxymethylcytosine DNA.
      IsotypeIgG1κ
      Species Reactivity
      • All
      Antibody TypeMonoclonal Antibody
      Purification MethodProtein G Purified
      Molecular WeightThis antibody detects 5-hydroxymethylcytosine.
      Product Usage Statements
      Quality AssuranceEvaluated by Dot Blot in unmodified and modified cytosine DNA.

      Dot Blot Analysis: 0.1 µg/mL of this antibody detected 5-hydroxymethylcytosine in 10 ng and 50 ng of unmodified and modified cytosine DNA.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µg
      Global Trade Item Number
      Catalogue Number GTIN
      MABE251 04053252691188