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GF021 Stem Cell Factor Protein, Recombinant human

The Stem Cell Factor (SCF) Protein, Recombinant human, is a hematopoietic growth factor that exerts its activity at the early stages of hematopoiesis.

Stem Cell Factor Protein, Recombinant human MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
UniProt Number: P21583 Entrez Gene Number: NM_000899.3, NM_003994.4
GF021
10 µg  
Purchase on Sigma-Aldrich

Overview

Replacement Information

Key Spec Table

SpeciesSourcePurity
Human E.coliGreater than 98% by SDS-PAGE and HPLC analyses. Endotoxin level is less than 0.1 ng per μg (1EU/μg) of SCF.
Description
Catalogue NumberGF021
Brand Family Chemicon®
Trade Name
  • Chemicon
DescriptionStem Cell Factor Protein, Recombinant human
OverviewStem Cell Factor (SCF) is a hematopoietic growth factor that exerts its activity at the early stages of hematopoiesis. SCF stimulates the proliferation of myeloid, erythroid, and lymphoid progenitors in bone marrow cultures and has been shown to act synergistically with colony stimulating factors. Recombinant human SCF is an 18.4 kDa protein containing 165 amino acid residues corresponding to the soluble secreted form of SCF.
Alternate Names
  • SCF
  • Mast Cell Growth Factor
  • MGF
  • c-kit Ligand
References
Product Information
HS Code3504 00 90
PresentationThe sterile filtered solution was lyophilized from 10mM Acetic acid.
Quality LevelMQ100
Applications
ApplicationThe Stem Cell Factor (SCF) Protein, Recombinant human, is a hematopoietic growth factor that exerts its activity at the early stages of hematopoiesis.
Application NotesFor most in vitro applications, SCF exerts its biological activity in the concentration range of 0.2 to 20.0 ng/mL. Responding cells are (partial list): Stem cells, BM precursor cells.
Biological Information
PurityGreater than 98% by SDS-PAGE and HPLC analyses. Endotoxin level is less than 0.1 ng per μg (1EU/μg) of SCF.
SourceE.coli
Specific ActivityChemicon's human SCF is fully biologically active when compared to standards. The ED50 was determined by the dose-dependent stimulation of the proliferation of human TF-1 cells is <= 2 ng/mL, corresponding to a specific activity of >= 5 x 10e5 units/mg.
Entrez Gene Number
Entrez Gene SummaryThis gene encodes the ligand of the tyrosine-kinase receptor encoded by the KIT locus. This ligand is a pleiotropic factor that acts in utero in germ cell and neural cell development, and hematopoiesis, all believed to reflect a role in cell migration. In adults, it functions pleiotropically, while mostly noted for its continued requirement in hematopoiesis. Two transcript variants encoding different isoforms have been found for this gene.
Gene Symbol
  • KITLG
  • DKFZp686F2250
  • SCF
  • SF
  • MGF
  • Kitl
  • KL-1
UniProt Number
UniProt SummaryFUNCTION: SwissProt: P21583 # Stimulates the proliferation of mast cells. Able to augment the proliferation of both myeloid and lymphoid hematopoietic progenitors in bone marrow culture. Mediates also cell-cell adhesion. Acts synergistically with other cytokines, probably interleukins.
SIZE: 273 amino acids; 30899 Da
SUBUNIT: Homodimer, non-covalently linked (Probable).
SUBCELLULAR LOCATION: Isoform 1: Cell membrane; Single-pass type I membrane protein (By similarity). Note=Also exists as a secreted soluble form (isoform 1 only) (By similarity). & Isoform 2: Cell membrane; Single-pass type I membrane protein (By similarity).
DEVELOPMENTAL STAGE: Acts in the early stages of hematopoiesis.
PTM: A soluble form is produced by proteolytic processing of isoform 1 in the extracellular domain.
SIMILARITY: SwissProt: P21583 ## Belongs to the SCF family.
Stem Cell Type
  • Hematopoietic Stem Cells
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsThe lyophilized powder, though stable for a few weeks at room temperature, is best stored at -20°C. Reconstitute in water to a concentration of 0.1-1.0 mg/mL. This solution can be further diluted into other aqueous buffers and stored at 2-8°C for 1 week or -20°C for future use. Reconstituted SCF should be stored in undiluted aliquots at -20°C. Avoid repeated freeze/thaw cycles.
Packaging Information
Material Size10 µg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
GF021 08436037124846

Documentation

Stem Cell Factor Protein, Recombinant human SDS

Title

Safety Data Sheet (SDS) 

Stem Cell Factor Protein, Recombinant human Certificates of Analysis

TitleLot Number
RECOMBINANT HUMAN STEM CELL FACTOR 3066210
RECOMBINANT HUMAN STEM CELL FACTOR 3089586
RECOMBINANT HUMAN STEM CELL FACTOR 2914553
RECOMBINANT HUMAN STEM CELL FACTOR 2475586
RECOMBINANT HUMAN STEM CELL FACTOR 3030265
RECOMBINANT HUMAN STEM CELL FACTOR 2952620
RECOMBINANT HUMAN STEM CELL FACTOR 3117787
RECOMBINANT HUMAN STEM CELL FACTOR 3050811
RECOMBINANT HUMAN STEM CELL FACTOR 3774184
RECOMBINANT HUMAN STEM CELL FACTOR - 2012882 2012882
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Data Sheet

Title
Reprogramming Cell Fate and Function Novel Strategies for iPSC Generation, Characterization, and Differentiation

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