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HTS100RTA Ready-to-Assay™ delta Opioid Receptor Frozen Cells

Human Delta / OP1 / DOP / DOR GPCR frozen cells for Calcium Flux FLIPR Assays.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Host Cell: Chem-1 Entrez Gene Number: NM_000911
HTS100RTA
1 kit  
Purchase on Sigma-Aldrich

Overview

Replacement Information

Key Spec Table

SpeciesHost CellsProtein Target
Human Chem-1Delta / OP1 / DOP / DOR
Description
Catalogue NumberHTS100RTA
Trade Name
  • Ready-to-Assay
DescriptionReady-to-Assay™ delta Opioid Receptor Frozen Cells
Background InformationMillipore’s Ready-to-Assay GPCR frozen cells are designed for simple, rapid calcium assays with no requirement for intensive cell culturing. Millipore has optimized the freezing conditions to provide cells with high viability and functionality post-thaw. The user simply thaws the cells and resuspends them in media, dispenses cell suspension into assay plates and, following over night recovery, assays for calcium response.

Opiates derived from the opium poppy, Papaver somniferum, have been used in for millenia for their anti-diarrheal, analgesic and euphoric properties. More recently, endogenous peptides, enkephalins, dynorphins, and endorphins, were found to bind to the same sites as opiate alkaloids. The receptors for the classical opioids are three related GPCRs, δ, κ, andμ also known as OP1, OP2 and OP3, respectively), that activate Gi/o to reduce intracellular cAMP levels. Although most clinically used opioids function by activation of the μ opioid receptor, agonist of spinal δ opioid receptors have antinociceptive activity that is independent of μ. In addition, activation of δ increases locomotor activity, inhibits gastrointestinal motility, and decreases respiratory frequency (Dhawan et al., 1996). Agonists for δ opioid receptors also exhibit antidepressant-like activity in animal models (Broom et al., 2002). Millipore’s cloned human δ opioid -expressing cell line is made in the Chem-1 host, which supports high levels of recombinant δ opioid expression on the cell surface and contains high levels of the promiscuous G protein Gα15 to couple the receptor to the calcium signaling pathway. Thus, the cell line is an ideal tool for screening for agonists, antagonists and modulators at δ.
References
Product Information
Components
  • Pack contains 2 vials of mycoplasma-free cells, 1 ml per vial.
  • Fifty (50) mL of Media Component.
Detection methodFluorescent
Quality LevelMQ100
Applications
ApplicationHuman Delta / OP1 / DOP / DOR GPCR frozen cells for Calcium Flux FLIPR Assays.
Key Applications
  • Calcium Flux Assays
Biological Information
Entrez Gene Number
GPCR ClassA
Host CellsChem-1
Protein TargetDelta / OP1 / DOP / DOR
Target Sub-FamilyOpioid
Cell Line Type
  • GPCR Cell Lines
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Usage Statement
  • This product contains genetically modified organisms (GMO). Within the EU GMOs are regulated by Directives 2001/18/EC and 2009/41/EC of the European Parliament and of the Council and their national implementation in the member States respectively. This legislation obliges Merck to request certain information about you and the establishment where the GMOs are being handled. Click here for Enduser Declaration (EUD) Form.
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
  • Limited to a single use and shall not be propagated and/or re-frozen by licensee.
Storage and Shipping Information
Packaging Information
Material Size1 kit
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
HTS100RTA 04053252518737

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