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553504 RAD51, His•Tag®, Pneumocystis carinii, Recombinant, E. coli

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
553504
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Overview

Replacement Information

Products

Catalogue NumberPackaging Qty/Pack
553504-10KU Plastic ampoule 10 ku
Description
OverviewRecombinant, Pneumocystis carinii RAD51 fused at the N-terminus to a His•Tag® sequence and expressed in E. coli. The Pneumocystis cariniiRAD51 is equivalent to bacterial RecA and the homolog of yeast RAD51. It plays a pivotal role in the homologous recombination pathway for DNA double-strand break (DSB) repair. RAD51 has been shown to colocalize with tumor suppressor proteins BRCA1 (Cat. No. 203659) and BRCA2 in DNA-damage-induced foci and RAD51 is known to interact directly with BRCA2.
Catalogue Number553504
Brand Family Calbiochem®
References
ReferencesWest, S.C. 2003. Nat. Rev. Mol. Cell Biol. 4, 435.
Jasin, M. 2002. Oncogene 21, 8981.
Symington, L.S., et al. 2002. Microbiol. Mol. Biol. Rev. 66, 630.
Daboussi, F., et al. 2002. Cell. Signal. 14, 969.
Liu, Y. and West, S.C. 2002. Breast Cancer Res. 4, 9.
Product Information
Unit of DefinitionOne unit equals 1 ng of purified protein. 20 units are sufficient for a DNA-protein assay and 100 units are sufficient for a protein-protein interaction assay.
FormLiquid
FormulationIn 100 mM KCl, 20 mM Tris-HCl, 1 mM DTT, 0.2 mM EDTA, 20% glycerol, pH 8.0.
Quality LevelMQ100
Applications
Biological Information
Purity≥95% by SDS-PAGE
Concentration Label Please refer to vial label for lot-specific concentration
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Dry Ice Only
Toxicity Standard Handling
Storage ≤ -70°C
Avoid freeze/thaw Avoid freeze/thaw
Do not freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-70°C).
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
553504-10KU 04055977193657

Documentation

RAD51, His•Tag®, Pneumocystis carinii, Recombinant, E. coli SDS

Title

Safety Data Sheet (SDS) 

RAD51, His•Tag®, Pneumocystis carinii, Recombinant, E. coli Certificates of Analysis

TitleLot Number
553504

References

Reference overview
West, S.C. 2003. Nat. Rev. Mol. Cell Biol. 4, 435.
Jasin, M. 2002. Oncogene 21, 8981.
Symington, L.S., et al. 2002. Microbiol. Mol. Biol. Rev. 66, 630.
Daboussi, F., et al. 2002. Cell. Signal. 14, 969.
Liu, Y. and West, S.C. 2002. Breast Cancer Res. 4, 9.
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision30-April-2016 JSW
DescriptionRecombinant, Pneumocystis carinii RAD51 fused at the N-terminus to a His•Tag® sequence and expressed in E. coli. The Pneumocystis carinii RAD51 is equivalent to bacterial RecA and the homologue of yeast RAD51. It plays a pivotal role in the homologous recombination pathway for DNA double-strand break (DSB) repair. RAD51 has been shown to colocalize with tumor suppressor proteins BRCA1 (Cat. No. 203659) and BRCA2 in DNA-damage-induced foci and RAD51 is known to interact directly with BRCA2.
FormLiquid
FormulationIn 100 mM KCl, 20 mM Tris-HCl, 1 mM DTT, 0.2 mM EDTA, 20% glycerol, pH 8.0.
Concentration Label Please refer to vial label for lot-specific concentration
Purity≥95% by SDS-PAGE
Unit definitionOne unit equals 1 ng of purified protein. 20 units are sufficient for a DNA-protein assay and 100 units are sufficient for a protein-protein interaction assay.
Storage Avoid freeze/thaw
≤ -70°C
Do Not Freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-70°C).
Toxicity Standard Handling
ReferencesWest, S.C. 2003. Nat. Rev. Mol. Cell Biol. 4, 435.
Jasin, M. 2002. Oncogene 21, 8981.
Symington, L.S., et al. 2002. Microbiol. Mol. Biol. Rev. 66, 630.
Daboussi, F., et al. 2002. Cell. Signal. 14, 969.
Liu, Y. and West, S.C. 2002. Breast Cancer Res. 4, 9.