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382159 PhosphoDetect™ Anti-Histone H3 (pSer¹⁰) (7-20) Rabbit pAb

This PhosphoDetect™ Anti-Histone H3 (pSer¹⁰) (7-20) Rabbit pAb is validated for use in Immunoblotting, ICC, IHC, IP for the detection of Histone H3 (pSer¹⁰) (7-20).

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382159
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Overview

Key Spec Table

Species ReactivityHostAntibody Type
Nem, Dr, H, T, XnRbPolyclonal Antibody

Products

Catalogue NumberPackaging Qty/Pack
382159-50UG Plastic ampoule 50 μg
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Description
OverviewRecognizes the ~17 kDa histone H3 protein phosphorylated at Ser10.
Catalogue Number382159
Brand Family Calbiochem®
Application Data
Detection of human histone H3, phosphorylated on Ser10, by immunoprecipitation. Sample: Acid extracted proteins from HeLa cells. Primary antibody: PhosphoDetect™ Anti-Histone H3 (pSer10) (7-20) Rabbit pAb (Cat. No. 382159) (assay dependent). Detection: chemiluminescence.
References
ReferencesAjiro, K., et al. 1996. J. Biol. Chem. 271, 13197.
Chadee, D.N., et al. 1995. J. Biol. Chem. 270, 20098.
Mahadevan, L.C., et al. 1991. Cell 65, 775.
Product Information
FormLiquid
FormulationIn 150 mM NaCl, 100 mM Tris-glycine, pH 7.4.
Positive controlAcid-extracted proteins from colcemid-arrested HeLa cells
Preservative≤0.1% sodium azide
Quality LevelMQ100
Applications
Key Applications Immunoblotting (Western Blotting)
Immunocytochemistry
Immunohistochemistry
Immunoprecipitation
Application NotesImmunoblotting (0.5 µg/ml)
Immunocytochemistry (5 µg/ml)
Immunohistochemistry (see comments)
Immunoprecipitation (4 µg/10 µg acid-extracted proteins from colcemid-arrested HeLa cells)
Application CommentsFor immunocytochemistry, colcemid-arrested HeLa cells were fixed with 1% paraformaldehyde. Also detects histone H3 in Tetrahymena micronuclei. The antibody has been reported to detect mitotic cells in Xenopus, Caenorhabditis elegans, and Drosophila embryos. Does not cross-react with unphosphorylated histone H3. This antibody has also been reported to work for immunohistochemistry. Variables associated with assay conditions will dictate the optimal working dilution.
Biological Information
Immunogena synthetic phosphopeptide [ARKpSTGGKAPRKQL(C)] corresponding to amino acids 7-20 surrounding the Ser¹⁰ phosphorylation site of human histone H3, conjugated to KLH
ImmunogenHuman
HostRabbit
IsotypeIgG
Species Reactivity
  • Nematode
  • Drosophila
  • Human
  • Tetrahymena
  • Xenopus
Antibody TypePolyclonal Antibody
Concentration Label Please refer to vial label for lot-specific concentration
Storage and Shipping Information
Ship Code Dry Ice Only
Toxicity Standard Handling
Storage -20°C
Avoid freeze/thaw Avoid freeze/thaw
Do not freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
Global Trade Item Number
Catalogue Number GTIN
382159-50UG 04055977213218