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516560 Pertussis Toxin, Bordetella pertussis - CAS 70323-44-3 - Calbiochem

This Pertussis toxin, CAS 70323-44-3, catalyzes ADP-ribosylation of guanine nucleotide-binding regulatory proteins Gi, Go, and Gt.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: Islet-activating Protein

516560
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Overview

Key Spec Table

CAS #
70323-44-3

Products

Catalogue NumberPackaging Qty/Pack
516560-50UG 50 μg
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Description
OverviewObtain a uniform suspension before use. A protein endotoxin that catalyzes ADP-ribosylation of guanine nucleotide-binding regulatory proteins Gi, Go, and Gt. Used in the study of adenylate cyclase regulation and the role of Gi proteins. Holotoxin activity is determined in a CHO cell assay.
NOTE: This product is not activated, hence for use in cell-free systems, activation is required.
Catalogue Number516560
Brand Family Calbiochem®
SynonymsIslet-activating Protein
References
ReferencesJoshi, S.A., et al. 1998. FEBS Lett. 441, 67.
Fryer, M.W. 1992. Neurosci. Lett. 146, 84.
Gierschik, P. 1992. Curr. Topics Microbiol. Immunol. 175, 69.
Harada, J., et al. 1992. Eur. J. Pharmacol. 227, 301.
Hausman, S.Z., and Burns, D.L. 1992. J. Biol. Chem. 267, 13735.
Kaslow, H.R., and Burns, D.L., 1992. FASEB J. 6, 2684.
Zhu, J., et al. 1992. J. Pharmacol. Exp. Therap. 263, 1479.
Hewlett, E.L., et al. 1983. Infect. Immun. 40, 1198.
Moss, J., et al. 1983. J. Biol. Chem. 258, 11879.
Product Information
CAS number70323-44-3
ATP CompetitiveN
FormLyophilized
FormulationLyophilized from 50 mM NaCl, 10 mM sodium phosphate buffer, pH 7.0.
ReversibleN
Quality LevelMQ100
Biological Information
Primary TargetGuanine nucleotide-binding regulatory proteins Gi, Go, and Gt
PurityFive distinct bands by SDS-PAGE
Physicochemical Information
Cell permeableN
ContaminantsAdenylate cyclase activity: ≤35 pmol/min/µg in the presence of calmodulin
Safety Information according to GHS
RTECSXW5883750
Safety Information
R PhraseR: 23/24/25

Toxic by inhalation, in contact with skin and if swallowed.
S PhraseS: 36/37/39-45

Wear suitable protective clothing, gloves and eye/face protection.
In case of accident or if you feel unwell, seek medical advice immediately (show the label where possible).
Storage and Shipping Information
Ship Code Ambient Temperature Only
Toxicity Toxic
Hazardous Materials Attention: Due to the nature of the Hazardous Materials in this shipment, additional shipping charges may be applied to your order. Certain sizes may be exempt from the additional hazardous materials shipping charges. Please contact your local sales office for more information regarding these charges.
Storage +2°C to +8°C
Do not freeze Yes
Special InstructionsFollowing reconstitution, refrigerate (4°C). For long-term storage in solution, reconstitute with a high ionic strength buffer, such as 100 mM sodium phosphate, 500 mM sodium chloride, pH 7.0. Stock solutions are stable for at least 6 months for use in CHO cell assays and for 1-2 months for use in non-cellular systems.
Global Trade Item Number
Catalogue Number GTIN
516560-50UG 04055977198157