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109253 Papanicolaou's solution 1a Harris' hematoxylin solution

for cytology

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: Hematoxylin solution acc. to Harris, Colour solution acc. to Papanicolaou, Harris' Hematoxylin solution

109253
Purchase on Sigma-Aldrich

Overview

Replacement Information

Products

Catalogue NumberPackaging Qty/Pack
1092530500 Plastic bottle 500 ml
1092531000 Plastic bottle 1 l
1092532500 Plastic bottle 2.5 l
Description
Catalogue Number109253
SynonymsHematoxylin solution acc. to Harris, Colour solution acc. to Papanicolaou, Harris' Hematoxylin solution
DescriptionPapanicolaou’s solution 1a Harris hematoxylin solution
OverviewPapanicolaou’s solution 1a Harris hematoxylin solution - for cytology, is a ready-to-use solution used for human-medical cell diagnosis and serve the purpose of the cytological investigation of sample material of human origin, for example, gynecological and clinico-cytological specimen materials. Papanicolaou’s technique is the most used staining procedure for cytological specimen is and is intended for the the staining of exfoliative cells in cytological specimens. In the first step, the cell nuclei are stained either progressively or regressively with a hematoxylin solution, (Hematoxylin solution modified acc.to Gill II, (Cat.no. 105175), Papanicolaou’s solution 1a Harris hematoxylin solution, (Cat.no. 109253) or Papanicolaou’s solution 1b Hematoxylin solution S, (Cat.no. 109254)). Nuclei are stained blue to dark violet. In the progressive hematoxylin staining method, staining is carried out to the endpoint, after which the slide is blued in tapwater. With the regressive method the material is over-stained and the excess of staining solution is removed by acid rinsing steps, followed by the bluing step. The structures of nuclei are more differentiated and better visible by the regressive method. The second staining step is cytoplasmic staining by (Papanicolaou’s solution 2b Orange II solution, (Cat. no. 1106887) or Papanicolaou’s solution 2a Orange G solution (OG6), (Cat. no. 106888)), especially for demonstration of mature and keratinized cells. The target structures are stained orange in different intensities. In the third staining step is used the so-called polychrome solution, a mixture of eosin, light green SF and Bismarck brown, Papanicolaou’s solution 3a polychromatic solution EA 31, (Cat.no 109271), Papanicolaou’s solution 3b polychromatic solution EA 50, (Cat.no. 109272), Papanicolaou’s solution 3c polychromatic solution EA 65,(Cat.no. 109270), or Papanicolaou’s solution 3d polychromatic solution EA 65, (Cat.no.109269). The polychrome solution is used for demonstration of differentiation of squamous cells. Both Papanicolaou’s solution 1a Harris hematoxylin solution(Cat.no. 109253) and Papanicolaou’s solution 1b Hematoxylin solution S (Cat.no. 109254) give a blue to dark violet staining result with clinical specimen material.
A 500 ml bottle of either solution is sufficient for 1500 -2500 stainings. It is registered as IVD and CE certified product and can be used for clinical diagnostics. For more details, please see instructions for use (IFU). The IFU can be downloaded from this webpage.
References
Product Information
HS Code3212 90 00
Quality LevelMQ400
Applications
Applicationfor cytology
Biological Information
Physicochemical Information
Boiling point100 °C (1013 hPa)
Density1.04 g/cm3 (20 °C)
pH value2.5 - 3.0 (H₂O, 20 °C)
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Hazard Pictogram(s) Hazard Pictogram(s)
Hazard Statement(s)H318: Causes serious eye damage.
EUH210: Safety data sheet available on request.
Precautionary Statement(s)P280: Wear eye protection/ face protection.
P305 + P351 + P338: IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.
Signal WordDanger
Storage class10 - 13 Other liquids and solids
WGKWGK 1 slightly hazardous to water
Disposal28
Aqueous solutions: Container D.
Safety Information
Product Usage Statements
Storage and Shipping Information
StorageStore at +15°C to +25°C.
Packaging Information
Transport Information
Supplemental Information
Specifications
Suitability for microscopy (Vaginal smear)passes test
Nucleiblue to dark violet
Cyanophilic Cytoplasm (basophilic)blue-green
Eosinophilic Cytoplasm (acidophilic)pink
Global Trade Item Number
Catalogue Number GTIN
1092530500 04022536112466
1092531000 04022536676661
1092532500 04022536112480

Documentation

Papanicolaou's solution 1a Harris' hematoxylin solution SDS

Title

Safety Data Sheet (SDS) 

Papanicolaou's solution 1a Harris' hematoxylin solution Certificates of Analysis

Product Number PackagingSpecificationLot Number
1092530500 Plastic bottlePDF Specification Document
1092531000 Plastic bottlePDF Specification Document
1092532500 Plastic bottlePDF Specification Document

User Guides

Title
User Guide (IFU) - 109253

Instructions for Use

Title
109253 Papanicolaous lösning 1a Harris’ hematoxylinlösning för cytologi

Related Products & Applications

Accessories (Primary)

Catalogue Number Description
106887 Papanicolaou’s solution 2b Orange II solution
106888 Papanicolaou's solution 2a Orange G solution (OG 6)
109254 Papanicolaou's solution 1b Hematoxylin solution S
109269 Papanicolaou's solution 3d polychromatic solution EA 65
109271 Papanicolaou's solution 3a polychromatic solution EA 31
109272 Papanicolaou's solution 3b polychromatic solution EA 50
115938 Hematoxylin monohydrate (C.I.75290)
109270 Papanicolaou's solution 3c Polychromatic solution EA 65

Accessories (Secondary)

Catalogue Number Description
104302 Hematoxylin cryst. (C.I. 75290)
105175 Hematoxylin solution modified acc. to Gill II
106887 Papanicolaou’s solution 2b Orange II solution
106888 Papanicolaou's solution 2a Orange G solution (OG 6)
109254 Papanicolaou's solution 1b Hematoxylin solution S
109269 Papanicolaou's solution 3d polychromatic solution EA 65
109271 Papanicolaou's solution 3a polychromatic solution EA 31
109272 Papanicolaou's solution 3b polychromatic solution EA 50
109270 Papanicolaou's solution 3c Polychromatic solution EA 65

Related Products By: Application Facete

Clinical Cytology
Cytodiagnosis
Papanicolaou

Categories

IVD/OEM Materials & Reagents > Microscopy > Cytology > Cytological Staining Reagents