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NS01L Normal Rabbit Serum

NS01L
Purchase on Sigma-Aldrich

Overview

Products

Catalogue NumberPackaging Qty/Pack
NS01L-1ML Plastic ampoule 1 ml
Description
OverviewNormal carrier sera from healthy, non-immunized rabbits.
Catalogue NumberNS01L
Brand Family Calbiochem®
Product Information
FormLyophilized
FormulationSerum diluted in 250 mM NaCl, 10 mM PBS, pH 7.6.
Preservative≤0.1% sodium azide
Applications
Key Applications Enzyme-Linked Immunosorbent Assay
Immunoblotting (Western Blotting)
Immunofluorescence
Immunohistochemistry
Immunoprecipitation
Application NotesELISA (see comments)
Flow Cytometry (see comments)
Immunoblotting (see comments)
Immunofluorescence (see comments)
Immunohistochemistry (see comments)
Immunoprecipitation (see comments)
Application CommentsNormal sera are provided to be used as blocking agents in immunohistochemical procedures or as negative controls for undiluted serum/rabbit polyclonal antibodies from the Calbiochem® brand. For use as a negative control, perform experiments in parallel using primary antibody and normal serum at the same concentrations. A signal obtained with the normal serum indicates a nonspecific interaction; any additional signal with the primary antibody indicates a specific antibody-antigen interaction. This antibody has also been reported to work for ELISA, flow cytometry, immunoblotting, immunofluorescence, immunohistochemistry, and immunoprecipitation.
Biological Information
HostRabbit
Storage and Shipping Information
Ship Code Ambient Temperature Only
Toxicity Standard Handling
Storage +2°C to +8°C
Do not freeze Ok to freeze
Special InstructionsReconstitute the lypohilized diluted serum in 1 ml sterile distilled water with occasional gentle mixing for at least 2 h at 4°C. Following reconstitution, aliquot and freeze (-20°C) for long-term storage or refrigerate (4°C) with 0.1% azide for short-term storage. Avoid freeze/thaw cycles of solutions.
Global Trade Item Number
Catalogue Number GTIN
NS01L-1ML 04055977210019