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14-545 NEK2 Protein, active, 10 µg

Active, N-terminal His6-tagged, recombinant, full-length, human NEK2, for use in Kinase Assays.

NEK2 Protein, active, 10 µg MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
14-545
10 µg  Also available in 250 µg size --call for pricing and availability and reference catalog number 14-545M when ordering the 250 µg size.
Purchase on Sigma-Aldrich

Overview

Replacement Information
Description
Catalogue Number14-545
Brand Family Upstate
Trade Name
  • Upstate
DescriptionNEK2 Protein, active, 10 µg
OverviewN-terminal His6-tagged, recombinant, full-length, human NEK2
References
Product Information
Quality LevelMQ100
Applications
ApplicationActive, N-terminal His6-tagged, recombinant, full-length, human NEK2, for use in Kinase Assays.
Key Applications
  • Kinase Assay
Biological Information
Sourceexpressed by baculovirus in Sf21 cells
Specific ActivityFor Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme.
Entrez Gene Number
Gene Symbol
  • NEK2
  • NLK1
  • NLK
  • NEK2A
  • HsPK21
Protein TargetNEK2
Purification MethodNi2+/NTA-agarose
Target Sub-FamilyMisc
UniProt Number
UniProt SummaryFUNCTION: SwissProt: P51955 # Protein kinase that is involved in mitotic regulation. May have a role at the G2-M transition. May also play a role in meiosis. Isoform 1 but not isoform 2 appears to play a role in centrosome splitting. Isoform 1 phosphorylates and activates NEK11 in G1/S-arrested cells. Isoform 2, which is not present in the nucleolus, does not.
COFACTOR: Magnesium.
SIZE: 445 amino acids; 51763 Da
SUBUNIT: Interacts with TERF1. Isoform 1 and isoform 2 form homo- and heterodimers.
SUBCELLULAR LOCATION: Nucleus. & Isoform 1: Nucleus, nucleolus. Note=Has a nucleolar targeting/ retention activity via a coiled-coil domain at the C-terminal end. & Isoform 2: Cytoplasm. Note=Predominantly cytoplasmic.
TISSUE SPECIFICITY: Isoform 1 and isoform 2 are expressed in peripheral blood T-cells and a wide variety of transformed cell types.
DEVELOPMENTAL STAGE: Accumulates throughout S phase and shows maximal levels in late G2. This expression pattern is highly reminiscent of that of A and B cyclins. Expression of both isoform 1 and isoform 2 is low in the G1 phase and increases in the S/G2 phases. Isoform 1 is absent from cells arrested in the G2/M prometaphase, whereas isoform 2 remains present.
SIMILARITY: SwissProt: P51955 ## Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. NIMA subfamily. & Contains 1 protein kinase domain.
Molecular Weight52.8kDa
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality Assuranceroutinely evaluated by phosphorylation of MBP
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions6 months at -70°C
Packaging Information
Material Size10 µg
Material PackageAlso available in 250 µg size --call for pricing and availability and reference catalog number 14-545M when ordering the 250 µg size.
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
14-545 04053252401626

Related Products & Applications

Alternative Packsize

Catalogue Number Description
14-545M NEK2 Protein, active, 250 µg

Product Families

Kinase & Phosphatase Products

Millipore offers a large portfolio of Kinases, Phophatases, Substrates, Inhibitors, Kits, reagents & tools for Signaling research. See below for a list of these products, in multiple pack sizes.
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Categories

Life Science Research > Proteins and Enzymes > Purified Kinases
Life Science Research > Drug Discovery and Development > Kinase & Phosphatase Screening > Purified Kinases