Mobius® Chrom 20 System
For Purification of Clinical and Process Scale Biologics
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For Purification of Clinical and Process Scale Biologics Less
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Overview
The Mobius® Chrom 20 System is a flexible, automated, single-use chromatography system that enables consistent and reliable separation and purification of mAbs, vaccines, plasma, and therapeutic proteins at clinical and process scales.
The Mobius® Chrom 20 System delivers optimal operational flexibility for processes up to 2000L with flow rates up to 20L/min and enhanced isocratic and gradient accuracy.
Single-Use Disposable Products
Easy to Use
Thanks to our unique clamshell and single-use Flexware® assemblies Design
User Friendly
with operator comfort and safe design
Safe & Reliable Process
With 100% single-use, gamma-irradiated Flexware® and high gradient accuracy
Flexible
Easy scale-down to lower column sizes, and ability to cover multiple scales and applications
Specifications
Mobius® Chrom 20 System and Flexware® Assemblies Specifications
General System specifications
| Specification | Chrom 20 System | |
| Flow Range | In gradient mode within accuracy specifications: 240-1200L/h In Isocratic mode: 24-1200L/h |
|
| Pumps | Model | Quattroflow 1200S x 2 |
| Maximum Pressure | 4 bar | |
| Valves | On/Off Valves | Inside Clamshell: Automatic Pneumatic piston Inlet Manifold: Automatic Pinch Valve for 5/8” ID |
| Valve control (after Bubble Trap) | Pneumatic piston with pressure regulator | |
Mechanical Specifications: System and Assemblies
| Specification | Mobius® Chrom 20 System | |
| System Dimensions | Pump & Base carts connected H x W x D in mm | 1727 x 1715 x 800 ± 20 mm (68 x 67.5 x 31.5 in.) |
| Net Weight | Base cart with Clamshell | 365 kg (805 lbs) |
| Pump cart with 2 Pumps and Manifold | 160 kg (353 lbs) | |
| Materials of Construction | Wetted Components USP<88> Class VI compliant material | |
| Tubing | Silicone | |
| Flexware® Clamshell Assembly | Pureflex™ Film, polyethylene fittings and silicone gaskets, polypropylene | |
| Pump Head | EPDM, Santoprene® and polypropylene | |
| Connectors | Polypropylene, polysulfone | |
| Flowmeter | Polysulfone and Hastelloy C22 electrodes | |
| UV, Conductivity and pH Sensor (MU) | Quartz, EPDM and stainless steel 316L | |
| UV, Conductivity and pH Sensor (SUC) | Polysulfone Quartz, EPDM and stainless steel 316L (pins only) | |
| Non-Wetted Components | ||
| Carts frame & panels | Stainless steel 304L | |
| Clamshell (rear) | POM | |
| Clamshell (front) | PMMA | |
| Valve Pads | Silicone | |
| Casters | 8 (4 free, 4 locking)Inner : Polyamide 6Rolling area : Polyurethane | |
| Connections | Inlets (10) | ¾" TC |
| Outlets, 3 Fractions + 1 waste | ¾" TC | |
| Filters | ¾" TC | |
| Column | ¾" TC | |
| Pneumatic | ¾" TC | |
Operating Specifications
| Specification | Mobius® Chrom 20 System | |
| Total Operating Time | Flexware® Assemblies | Validated for 24 hours operation 50 cycles (50 valve openings and 50 valve closings) per valve The integrity of the installation may be compromised if used for more than 50 cycles |
| Valve Pads | Do not exceed 1200 cycles or 6 months of operation | |
| Product Temperature Range | 20 to 30°C (68 to 86°F) | |
| Maximum Pressure | Pump 1 Manifold | 0 to 2 bar (0 to 29 psi) |
| Pump 2 Manifold | 0 to 2 bar (0 to 29 psi) | |
| Pump Assembly | 0 to 4 bar (0 to 58 psi) | |
| Bubble Trap Assembly | 0 to 4 bar (0 to 58 psi) | |
| Clamshell assembly | 0 to 4 bar (0 to 58 psi) | |
| Precolumn Filter Assembly | 0 to 4 bar (0 to 58 psi) | |
| Pre-column Instrumentation Assembly | 0 to 4 bar (0 to 58 psi) | |
| Post-column Instrumentation Assembly | 0 to 2 bar (0 to 29 psi) | |
| Column Assembly | 0 to 4 bar (0 to 58 psi) | |
| System Operating Temperature | 20 to 30°C (68 to 86°F) | |
| Operating Humidity | 10 to 90% (non condensing) | |
Single-Use Disposable Products
| Specification | Mobius® Chrom 20 System |
| Power Supply | 220-240VAC, 50/60 Hz, 1 phase, 6.5 A max or 100–120VAC, 50/60 Hz, 1 phase, 13.6 A max Maximum consumption 1.6 KW |
| Pneumatic Air Supply | 6 bar (87 psi) min, 10 bar (145 psi) maximum, oil free Maximum consumption 4 L/min |
Single-Use Disposable Products
| Specification | Tag* | Range/Setting/Type/Accuracy | Process Connection |
| Pressure Indicators | PIT001PIT002PT003 | 0–4 bar (0-58 psi) ± 0.2 bar (2.9 psi) | Non-intrusive |
| High Pressure Switches | PSH001PSH002 | 4.2 bar (60 psi) | Non-intrusive |
| Single-Use Magnetic Flowmeters | FT001FT002 | ± 2% MV between0,4 L/min and 20 L/min | In-line |
| Temperature Sensors | TE201 | 2–30°C (35.6°F to 86°F) ± 1°C (33.8°F) | Combined with conductivity sensor |
| Single-use Conductivity Sensors | AE102AE201AAE201B | Process: 0-100 mS/cm ±2% FSCleaning: 0–50 μS/cm ±2% FS | In-line |
| pH Sensors | AE103AE202 | 1–14 pH ±0.1 pH | In-line |
| Air Sensors | XS001XS002 | N/A | Non-intrusive |
| UV Sensors | AE203/204 | AF45 : 0-3 AU ±2% FSOPL: 1 or 2.5 mmWavelength: 280 nm AF46 : 0-2 AU ±2% FSOPL: 1 or 2.5 mmWavelength: Dual 254/280 nm or 280/300 nm |
|
| Do not use UV Sensors in a condensing atmosphere. Condensation may lead to erroneous sensor readings. | |||
Ordering Information
Documentation
Related Products & Applications
Mobius® Chrom 20 System and Flexware® Assemblies Specifications
General System specifications
| Specification | Chrom 20 System | |
| Flow Range | In gradient mode within accuracy specifications: 240-1200L/h In Isocratic mode: 24-1200L/h |
|
| Pumps | Model | Quattroflow 1200S x 2 |
| Maximum Pressure | 4 bar | |
| Valves | On/Off Valves | Inside Clamshell: Automatic Pneumatic piston Inlet Manifold: Automatic Pinch Valve for 5/8” ID |
| Valve control (after Bubble Trap) | Pneumatic piston with pressure regulator | |
Mechanical Specifications: System and Assemblies
| Specification | Mobius® Chrom 20 System | |
| System Dimensions | Pump & Base carts connected H x W x D in mm | 1727 x 1715 x 800 ± 20 mm (68 x 67.5 x 31.5 in.) |
| Net Weight | Base cart with Clamshell | 365 kg (805 lbs) |
| Pump cart with 2 Pumps and Manifold | 160 kg (353 lbs) | |
| Materials of Construction | Wetted Components USP<88> Class VI compliant material | |
| Tubing | Silicone | |
| Flexware® Clamshell Assembly | Pureflex™ Film, polyethylene fittings and silicone gaskets, polypropylene | |
| Pump Head | EPDM, Santoprene® and polypropylene | |
| Connectors | Polypropylene, polysulfone | |
| Flowmeter | Polysulfone and Hastelloy C22 electrodes | |
| UV, Conductivity and pH Sensor (MU) | Quartz, EPDM and stainless steel 316L | |
| UV, Conductivity and pH Sensor (SUC) | Polysulfone Quartz, EPDM and stainless steel 316L (pins only) | |
| Non-Wetted Components | ||
| Carts frame & panels | Stainless steel 304L | |
| Clamshell (rear) | POM | |
| Clamshell (front) | PMMA | |
| Valve Pads | Silicone | |
| Casters | 8 (4 free, 4 locking)Inner : Polyamide 6Rolling area : Polyurethane | |
| Connections | Inlets (10) | ¾" TC |
| Outlets, 3 Fractions + 1 waste | ¾" TC | |
| Filters | ¾" TC | |
| Column | ¾" TC | |
| Pneumatic | ¾" TC | |
Operating Specifications
| Specification | Mobius® Chrom 20 System | |
| Total Operating Time | Flexware® Assemblies | Validated for 24 hours operation 50 cycles (50 valve openings and 50 valve closings) per valve The integrity of the installation may be compromised if used for more than 50 cycles |
| Valve Pads | Do not exceed 1200 cycles or 6 months of operation | |
| Product Temperature Range | 20 to 30°C (68 to 86°F) | |
| Maximum Pressure | Pump 1 Manifold | 0 to 2 bar (0 to 29 psi) |
| Pump 2 Manifold | 0 to 2 bar (0 to 29 psi) | |
| Pump Assembly | 0 to 4 bar (0 to 58 psi) | |
| Bubble Trap Assembly | 0 to 4 bar (0 to 58 psi) | |
| Clamshell assembly | 0 to 4 bar (0 to 58 psi) | |
| Precolumn Filter Assembly | 0 to 4 bar (0 to 58 psi) | |
| Pre-column Instrumentation Assembly | 0 to 4 bar (0 to 58 psi) | |
| Post-column Instrumentation Assembly | 0 to 2 bar (0 to 29 psi) | |
| Column Assembly | 0 to 4 bar (0 to 58 psi) | |
| System Operating Temperature | 20 to 30°C (68 to 86°F) | |
| Operating Humidity | 10 to 90% (non condensing) | |
Single-Use Disposable Products
| Specification | Mobius® Chrom 20 System |
| Power Supply | 220-240VAC, 50/60 Hz, 1 phase, 6.5 A max or 100–120VAC, 50/60 Hz, 1 phase, 13.6 A max Maximum consumption 1.6 KW |
| Pneumatic Air Supply | 6 bar (87 psi) min, 10 bar (145 psi) maximum, oil free Maximum consumption 4 L/min |
Single-Use Disposable Products
| Specification | Tag* | Range/Setting/Type/Accuracy | Process Connection |
| Pressure Indicators | PIT001PIT002PT003 | 0–4 bar (0-58 psi) ± 0.2 bar (2.9 psi) | Non-intrusive |
| High Pressure Switches | PSH001PSH002 | 4.2 bar (60 psi) | Non-intrusive |
| Single-Use Magnetic Flowmeters | FT001FT002 | ± 2% MV between0,4 L/min and 20 L/min | In-line |
| Temperature Sensors | TE201 | 2–30°C (35.6°F to 86°F) ± 1°C (33.8°F) | Combined with conductivity sensor |
| Single-use Conductivity Sensors | AE102AE201AAE201B | Process: 0-100 mS/cm ±2% FSCleaning: 0–50 μS/cm ±2% FS | In-line |
| pH Sensors | AE103AE202 | 1–14 pH ±0.1 pH | In-line |
| Air Sensors | XS001XS002 | N/A | Non-intrusive |
| UV Sensors | AE203/204 | AF45 : 0-3 AU ±2% FSOPL: 1 or 2.5 mmWavelength: 280 nm AF46 : 0-2 AU ±2% FSOPL: 1 or 2.5 mmWavelength: Dual 254/280 nm or 280/300 nm |
|
| Do not use UV Sensors in a condensing atmosphere. Condensation may lead to erroneous sensor readings. | |||