Millipore Sigma Vibrant Logo
Attention: We have moved. Merck Millipore products are no longer available for purchase on MerckMillipore.com.Learn More

MAGE1MAG-25K MILLIPLEX® MAP Mouse Aging Magnetic Bead Panel 1

The analytes available for this multiplex kit are: CTACK/CCL27, FGF-21, GnRH, IL-6, Jag1, Leptin, Notch1, TNFα.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Key Applications: Multiplexing Detection Methods: Luminex xMAP Research Category: Metabolism Research Sub-Category: Aging, Immune Response, Metabolism
MAGE1MAG-25K
Purchase on Sigma-Aldrich

Overview

Replacement Information

Key Spec Table

Analytes AvailableSpecies ReactivityKey ApplicationsDetection Methods
CTACK/CCL27 FGF-21 GnRH IL-6 Jag1 Leptin Notch1 TNF-α MMplexLuminex xMAP
Description
Catalogue NumberMAGE1MAG-25K
Trade Name
  • MILLIPLEX
DescriptionMILLIPLEX® MAP Mouse Aging Magnetic Bead Panel 1
OverviewThe MILLIPLEX® MAP Mouse Aging Magnetic Bead Panel 1 is an 8-plex kit to be used for the simultaneous quantification of any or all of the following analytes in mouse serum, plasma and cell/tissue culture samples: C-C Motif Chemokine 27 (CTACK), Fibroblast Growth Factor 21 (FGF21), Progonadoliberin (GnRH), Interleukin-6 (IL-6), Protein Jagged-1 (Jagged1), Leptin, Neurogenic Locus Notch Homolog Protein 1 (Notch1), and Tumor Necrosis Factor Alpha (TNFα).
Background InformationMice are widely used models for both aging and senescence because of their similarity to
humans. Aging associated physiological changes and the pathological consequences of these
changes make the elderly population vulnerable to many chronic diseases, such as cancer, heart
disease, arthritis, osteoporosis, and cognitive decline. Many of these conditions have been
modelled in mice. Over the years, intensive research of model organisms has provided
fundamental evidence that aging can be delayed, and longevity extended. These findings gave
rise to a new era in aging research aiming to elucidate the underlying mechanisms that drive the
aging process.
The MILLIPLEX® MAP Mouse Aging Magnetic Bead Panel 1 covers multifaceted biomarkers of
aging-related conditions. Monitoring these novel biomarkers helps researchers to further
understand the complex biological process which may lead to new therapies postponing age-related diseases.
References
Product Information
Detection methodLuminex xMAP
ConfigurationDesign your multiplex kit by choosing available analytes within this panel.
Precision, %
  • CTACK/CCL27: Intra-Assay <10% CV; Inter-Assay <20% CV
    FGF-21: Intra-Assay <10% CV; Inter-Assay <20% CV
    GnRH: Intra-Assay <10% CV; Inter-Assay <20% CV
    IL-6: Intra-Assay <10% CV; Inter-Assay <20% CV
    Jag1: Intra-Assay <10% CV; Inter-Assay <20% CV
    Leptin: Intra-Assay <10% CV; Inter-Assay <20% CV
    Notch1: Intra-Assay <10% CV; Inter-Assay <20% CV
    TNFα: Intra-Assay <10% CV; Inter-Assay <20% CV
Panel TypeMAGNETIC Metabolism
Applications
ApplicationThe analytes available for this multiplex kit are: CTACK/CCL27, FGF-21, GnRH, IL-6, Jag1, Leptin, Notch1, TNFα.
Key Applications
  • Multiplexing
Application NotesFor optimal sensitivity, use an overnight (16-18 hour) incubation. Serum and plasma samples require (recommend) a 1:2 dilution with Assay Buffer. Tissue culture supernatants may be run neat or appropriately diluted in control medium. Each well requires 25 μL of sample, standard or control.
Biological Information
SpecificityThere was no or negligible cross-reactivity between the antibodies for an analyte and any of the other analytes in this panel.
Species Reactivity
  • Mouse
Analytes Available
  • CTACK/CCL27
  • FGF-21
  • GnRH
  • IL-6
  • Jag1
  • Leptin
  • Notch1
  • TNF-α
Cross ReactivityThere was no or negligible cross-reactivity between the antibodies for an analyte and any of the other analytes in this panel.
Accuracy
  • CTACK/CCL27 95%
    FGF-21 100%
    GnRH 93%
    IL-6 98%
    Jag1 101%
    Leptin 96%
    Notch1 103%
    TNFα 106%
Physicochemical Information
Sensitivity
  • CTACK/CCL27: 0.7 pg/mL
    FGF-21: 5.6
    GnRH: 1.8
    IL-6: 3.3
    Jag1: 90.0
    Leptin: 3.4
    Notch1: 76.0
    TNFα: 2.4.
Standard Curve Range
  • CTACK/CCL27 0.49 – 2,000 pg/mL
    FGF-21 5.86 – 24,000 pg/mL
    GnRH 1.95 – 8,000 pg/mL
    IL-6 5.86 – 24,000 pg/mL
    Jag1 195 – 800,000 pg/mL
    Leptin 2.93 – 12,000 pg/mL
    Notch1 97.7 – 400,000 pg/mL
    TNFα 1.95 – 8,000 pg/mL
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsRecommended storage for kit components is 2 - 8°C.
Packaging Information
Material Size96-well plate
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
MAGE1MAG-25K 04061841153955