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EZHPI-15K Human Total Proinsulin ELISA

This Human Total Proinsulin ELISA is used to measure & quantify Proinsulin levels in Metabolism & Endocrine & Cell Signaling research.

Human Total Proinsulin ELISA MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Key Applications: ELISA Detection Methods: Fluorescent, Colorimetric Research Category: Metabolism Research Sub-Category: Obesity, Metabolic Disorders
EZHPI-15K
96-well strip plate  
Purchase on Sigma-Aldrich

Overview

Replacement Information

Key Spec Table

Analytes AvailableSpecies ReactivityKey ApplicationsDetection Methods
Proinsulin HELISAFluorescent Colorimetric
Description
Catalogue NumberEZHPI-15K
DescriptionHuman Total Proinsulin ELISA
Background InformationProinsulin, the precursor of insulin and C-peptide, is made by the beta cells of the pancreas. This kit measures the intact and intermediate metabolic forms of human proinsulin.
References
Product Information
Detection methodFluorescent Colorimetric
HS Code3002 15 90
Precision, %
  • Inter-assay: 2.9–8.3
  • Intra-assay: 0.8–8.5
Quality LevelMQ200
Applications
ApplicationThis Human Total Proinsulin ELISA is used to measure & quantify Proinsulin levels in Metabolism & Endocrine & Cell Signaling research.
Key Applications
  • ELISA
Application Notes20 μL sample volume 3 hour assay, room temperature
Biological Information
Species Reactivity
  • Human
Analytes Available
  • Proinsulin
Entrez Gene Number
Entrez Gene SummaryAfter removal of the precursor signal peptide, proinsulin is post-translationally cleaved into two chains (peptide A and peptide B) that are covalently linked via two disulfide bonds. Binding of this mature form of insulin to the insulin receptor (INSR) stimulates glucose uptake. A variety of mutant alleles with changes in the coding region have been identified. There is a read-through gene, INS-IGF2, which overlaps with this gene at the 5' region and with the IGF2 gene at the 3' region.
Gene Symbol
  • INS
  • proinsulin
  • insulin
UniProt Number
UniProt SummaryFUNCTION: SwissProt: P01308 # Insulin decreases blood glucose concentration. It increases cell permeability to monosaccharides, amino acids and fatty acids. It accelerates glycolysis, the pentose phosphate cycle, and glycogen synthesis in liver.
SIZE: 110 amino acids; 11981 Da
SUBUNIT: Heterodimer of a B chain and an A chain linked by two disulfide bonds.
SUBCELLULAR LOCATION: Secreted.
DISEASE:SwissProt: P01308 # Defects in INS are the cause of familial hyperproinsulinemia [MIM:176730].
SIMILARITY: SwissProt: P01308 ## Belongs to the insulin family.
Accuracy
  • 88–103%
Physicochemical Information
Sensitivity
  • 0.5 pM
Linearity of Dilution84–120%
Standard Curve Range
  • 2–200 pM
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Usage Statement
  • For research use only. Not for use in diagnostic procedures.
Storage and Shipping Information
Packaging Information
Material Size96-well strip plate
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
EZHPI-15K 04053252320965