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SCC251 HL-60 DeltaF508-CF Human Promyelocytic Cell Line

HL-60 DeltaF508-CF human promyelocytic cell line is a valuable tool for drug screening and investigating the role of phagocytic defects in CF disease onset and progression.

SCC251
≥1X10⁶ cells/vial   
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Overview

Replacement Information
Description
Catalogue NumberSCC251
DescriptionHL-60 DeltaF508-CF Human Promyelocytic Cell Line
Alternate Names
  • DeltaF508-CF HL-60
Background InformationCystic fibrosis (CF) is caused by mutations in the CFTR gene, which encodes a chloride and bicarbonate ion channel (1). Among the approximately 150 known disease-causing mutations in CFTR, deletion of the phenylalanine-508 codon (ΔF508) is the most common and results in protein destabilization, misfolding and ultimately degradation (2). Although the most pronounced lung disease symptoms of CF, including chronic bacterial infection and purulent airway obstruction, are highly associated with neutrophils, the lack of cellular models for CF neutrophils has hindered progress in understanding the significance of these cells for CF pathogenesis (3).

The HL-60 DeltaF508-CF human promyelocytic cell line is a derivative of the HL-60 promyelocytic leukemia cell line that has been edited via CRISPR/Cas9 technology for homozygous deletion of CFTR F508 (3). Differentiation of HL-60 DeltaF508-CF cells into neutrophils occurs upon treatment with DMSO. Differentiated HL-60 DeltaF508-CF cells demonstrate normal levels of CD11b staining but substantially reduced CFTR surface expression compared to differentiated HL-60 control cells, in addition to compromised bactericidal activity (3).

Source:
The HL-60 DeltaF508-CF human promyelocytic cell line was derived from HL-60 promyelocytic leukemia cells transfected with a hCFTR-Cas9-eGFP plasmid. Single-cell clones were sorted for eGFP expression and screened for homozygous deletion of CFTR F508 via nuclease digestion and DNA sequencing (3).

References:
1. Nat Rev Dis Primers. 2015; 1:15010.0
2. Science. 2010; 329(5993): 805-810.
3. J Cyst Fibros. 2019; 18(1): 44-53.
References
Product Information
Applications
ApplicationHL-60 DeltaF508-CF human promyelocytic cell line is a valuable tool for drug screening and investigating the role of phagocytic defects in CF disease onset and progression.
Key Applications
  • Cell Culture
Application NotesThis product is intended for sale and sold solely to academic institutions for internal academic research use per the terms of the “Academic Use Agreement” as detailed in the product documentation. For information regarding any other use, please contact licensing@emdmillipore.com.
Biological Information
Cell Line Type
  • Cancer Cells
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality Assurance• Each vial contains ≥ 1X10⁶ viable cells.
• Cells are tested negative for infectious diseases by a Human Essential CLEAR panel by Charles River Animal Diagnostic Services.
• Cells are verified to be of human origin and negative for inter-species contamination from rat, mouse, chinese hamster, Golden Syrian hamster, and non-human primate (NHP) as assessed by a Contamination CLEAR panel by Charles River Animal Diagnostic Services.
• Cells are negative for mycoplasma contamination.
• Each lot of cells is genotyped by STR analysis to verify the unique identity of the cell line.
Usage Statement
  • This product contains genetically modified organisms (GMO). Within the EU GMOs are regulated by Directives 2001/18/EC and 2009/41/EC of the European Parliament and of the Council and their national implementation in the member States respectively. This legislation obliges Merck to request certain information about you and the establishment where the GMOs are being handled. Click here for Enduser Declaration (EUD) Form.
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStore in liquid nitrogen. The cells can be cultured for at least 10 passages after initial thawing without significantly affecting the cell marker expression and functionality.
Packaging Information
Material Size≥1X10⁶ cells/vial
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
SCC251 04061841689287