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03-186 HDAC8 human recombinant

Human recombinant HDAC8 expressed in E. Coli.

HDAC8 human recombinant MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Key Applications: Enzyme Assays
03-186
100 Units  
Purchase on Sigma-Aldrich

Overview

Replacement Information

Key Spec Table

Key ApplicationsSpecies
Enzyme AssaysHuman
Description
Catalogue Number03-186
Brand Family Upstate
Trade Name
  • Upstate
DescriptionHDAC8 human recombinant
OverviewHuman recombinant HDAC8 expressed in E. Coli
Background InformationHDAC8 is phosphorylated in vivo by PKA, resulting in decreased activity and hyperacetylation of HDAC8 targets, such as histones H3 and H4. In addition, HDAC8 can also be inhibited by trichostatin A. HDAC8 has been implicated in acute myeloid leukemia.
References
Product Information
FormatPurified
PresentationIn liquid containing 10 mM Tris, pH 7.5, 100 mM NaCl, 3 mM MgCl2 and 10% glycerol. Frozen solution.

Unit Definition: 1U = 1 pmol/min at 37°C using a standard flourometric activity assay to measure deacetylation activity
Quality LevelMQ300
Applications
ApplicationHuman recombinant HDAC8 expressed in E. Coli.
Key Applications
  • Enzyme Assays
Application NotesHDAC deacetylase assays
Biological Information
HostE. coli
PurityPurity >50% by SDS-PAGE
Specific Activity[Varies with lot. For Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme.]
Molecular Weight~42 kDa
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStore at -80°C. The enzyme is stable on ice for 30 to 60 min, but may lose activity with dilution and/or prolonged storage on ice. It is recommended that thawing and addition of the enzyme to assay wells be done within as short a time as possible before start of the assay and that the thawed enzyme be kept on ice throughout. Best performance will be obtained by adding the chilled, undiluted enzyme directly to pre-warmed buffer and proceeding immediately to the addition of pre-warmed substrate. The remaining, unused enzyme should be refrozen quickly by, for example, snap freezing in a dry/ice ethanol bath or liquid nitrogen. The enzyme is stable to at least 4 freeze/thaw cycles. To minimize the number of freeze/thaw cycles, aliquot the HDAC8 into separate tubes before storing at -80°C.
Packaging Information
Material Size100 Units
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
03-186 04053252550805

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