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345731
Sigma-AldrichGDF-5, Mouse, Recombinant
GDF-5, Mouse, Recombinant, consists of amino acids 376-495 and is expressed in E. coli with and without an N-terminal methionine. Involved in myogenesis, bone morphogenesis & neuron differentiatio
More>>GDF-5, Mouse, Recombinant, consists of amino acids 376-495 and is expressed in E. coli with and without an N-terminal methionine. Involved in myogenesis, bone morphogenesis & neuron differentiation. Less<<
GDF-5, Mouse, Recombinant MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Recombinant, mouse growth differentiation factor 5 (GDF-5) consisting of amino acids 376-495, expressed in E. coli with and without an N-terminal methionine. GDF-5, a member of the bone morphogenetic protein family, is involved in regulation of myogenesis, bone morphogenesis, neuron differentiation and survival, and chondrogenesis regulation.
Catalogue Number
345731
Brand Family
Calbiochem®
Synonyms
Cartilage-derived morphogenetic protein 1 (CDMP-1)
References
References
Settle, S.H., Jr., et al. 2003. Dev. Biol.254, 116. Massague, J., et al. 2000. Genes and Dev.14, 627. FrancisWest, P.H., et al. 1999. Development126, 1035.
Inada, M., et al. 1996. BBRC222, 317. Nishitoh, H., et al. 1996. J. Biol. Chem.271, 21345. Storm, E.E., et al. 1994. Nature368, 639.
Product Information
Form
Solid
Formulation
Lyophilized from 0.2 µm filter-sterilized Acetonitrile, TFA, BSA.
GDF-5, Mouse, Recombinant, consists of amino acids 376-495 and is expressed in E. coli with and without an N-terminal methionine. Involved in myogenesis, bone morphogenesis & neuron differentiation.
Biological Information
Biological activity
ED₅₀ = 1-4 µg/ml as measured by induction of alkaline phosphatase production by ATDC5 mouse chondrogenic cells. (Nakamura, K. et al. 1999. Exp. Cell Res.250, 351.)
Purity
≥95% by SDS-PAGE
Physicochemical Information
Contaminants
Endotoxin: <1.0 EU/µg protein
Storage and Shipping Information
Ship Code
Ambient Temperature Only
Toxicity
Standard Handling
Storage
-20°C
Do not freeze
Ok to freeze
Special Instructions
Following reconstitution refrigerate (4°C) for short-term storage or aliquot and freeze (-20°C) for long-term storage. Stock solutions are stable for up to 1 month at 4°C or for up to 3 months at -20°C.
GDF-5, Mouse, Recombinant Certificates of Analysis
Title
Lot Number
345731
References
Reference overview
Settle, S.H., Jr., et al. 2003. Dev. Biol.254, 116. Massague, J., et al. 2000. Genes and Dev.14, 627. FrancisWest, P.H., et al. 1999. Development126, 1035.
Inada, M., et al. 1996. BBRC222, 317. Nishitoh, H., et al. 1996. J. Biol. Chem.271, 21345. Storm, E.E., et al. 1994. Nature368, 639.
Data Sheet
Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.
Revision
13-September-2011 RFH
Synonyms
Cartilage-derived morphogenetic protein 1 (CDMP-1)
Description
Recombinant, mouse growth differentiation factor 5 (GDF-5) consisting of amino acids 376-495, expressed in E. coli with and without an N-terminal methionine. GDF-5, a member of the bone morphogenetic protein family, is involved in regulation of myogenesis, bone morphogenesis, neuron differentiation and survival, and chondrogenesis regulation.
Form
Solid
Formulation
Lyophilized from 0.2 µm filter-sterilized Acetonitrile, TFA, BSA.
Purity
≥95% by SDS-PAGE
Contaminants
Endotoxin: <1.0 EU/µg protein
Biological activity
ED₅₀ = 1-4 µg/ml as measured by induction of alkaline phosphatase production by ATDC5 mouse chondrogenic cells. (Nakamura, K. et al. 1999. Exp. Cell Res.250, 351.)
Solubility
Reconstitute to 150 µg/ml in sterile 4 mM HCl containing at least 0.1% human or bovine serum albumin.
Storage
-20°C
Do Not Freeze
Ok to freeze
Special Instructions
Following reconstitution refrigerate (4°C) for short-term storage or aliquot and freeze (-20°C) for long-term storage. Stock solutions are stable for up to 1 month at 4°C or for up to 3 months at -20°C.
Toxicity
Standard Handling
References
Settle, S.H., Jr., et al. 2003. Dev. Biol.254, 116. Massague, J., et al. 2000. Genes and Dev.14, 627. FrancisWest, P.H., et al. 1999. Development126, 1035.
Inada, M., et al. 1996. BBRC222, 317. Nishitoh, H., et al. 1996. J. Biol. Chem.271, 21345. Storm, E.E., et al. 1994. Nature368, 639.
