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EPA100 EPA Peroxidase Protection Buffer

500 mL EPA Peroxidase Protection Buffer for ELISA.

EPA Peroxidase Protection Buffer MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Key Applications: ELISA
EPA100
500 mL  
Purchase on Sigma-Aldrich

Overview

Replacement Information

Key Spec Table

Key Applications
ELISA
Description
Catalogue NumberEPA100
Brand Family Chemicon®
Trade Name
  • Chemicon
DescriptionEPA Peroxidase Protection Buffer
OverviewPeroxidase Protective Buffer is a diluent formulated to stabilize the activity of Chemicon horseradish peroxidase (HRP)-antibody conjugates at high conjugate dilution for extended periods.
References
Product Information
PresentationPeroxidase Protective Buffer contains 0.5% (w/v) bovine serum albumin, antimicrobial preservatives and stabilizing compounds in phosphate buffered saline, pH 7.2.
Applications
Application500 mL EPA Peroxidase Protection Buffer for ELISA.
Key Applications
  • ELISA
Application NotesUSE:

Chemicon HRP conjugated antibodies may be diluted up to 1:16,000 in Peroxidase Protective Buffer. Diluted conjugates are stable for at least 3 months when stored at 2 - 8oC. The buffer is suitable for direct use in ELISA. It is recommended that the HRP conjugated antibody is diluted with Peroxidase Protective Buffer and allowed to stand for 24 hours at 2-8°C to attain constant stability before use.

Peroxidase Protective Buffer is compatible with blocking agents such as skim milk powder, Tween 20 and normal serum. However HRP conjugates should not be stored in the presence of these blocking agents. They should be added just prior to use in the assay.
Biological Information
Physicochemical Information
Dimensions
Volume500 mL
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStore at +2 to +8°C, for up to 6 months.

PRECAUTIONS

HRP conjugates should be diluted and stored under clean conditions to reduce the level of microbial challenge.

Sodium azide must NOT be added to Peroxidase Protective Buffer.

Avoid contamination of HRP conjugate solutions with heavy metal ions, aromatic chlorocarbons or hypochlorous acids. Avoid containers made of soda glass or polystyrene. Storage of diluted conjugates in borosilicate glass is recommended.

Efficient washing steps should follow HRP conjugate incubations. In ELISA, at least four thorough wash cycles are recommended.
Packaging Information
Material Size500 mL
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
EPA100 04053252678509

Documentation

EPA Peroxidase Protection Buffer SDS

Title

Safety Data Sheet (SDS) 

EPA Peroxidase Protection Buffer Certificates of Analysis

TitleLot Number
EPA100 - 08051489354 08051489354
PEROXIDASE PROTECTIVE BUFFER -
PEROXIDASE PROTECTIVE BUFFER - 3274769 3274769
PEROXIDASE PROTECTIVE BUFFER - 3466088 3466088
PEROXIDASE PROTECTIVE BUFFER - 3607348 3607348
PEROXIDASE PROTECTIVE BUFFER - 3814362 3814362
PEROXIDASE PROTECTIVE BUFFER - 3853990 3853990
PEROXIDASE PROTECTIVE BUFFER - 4135719 4135719
PEROXIDASE PROTECTIVE BUFFER -2561040 2561040
PEROXIDASE PROTECTIVE BUFFER -2631125 2631125
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Data Sheet

Title
PEROXIDASE PROTECTIVE BUFFER CERTIFICATE OF ANALYSIS