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SCM162 DMEM/F-12 PLUS Basal Medium

Advanced DMEMF12 basal media used in low-serum or serum-free media formulations including stem cell and 3D organoid cell cultures.

SCM162
500 ml  
Purchase on Sigma-Aldrich

Overview

Description
Catalogue NumberSCM162
DescriptionDMEM/F-12 PLUS Basal Medium
Alternate Names
  • Organoid Basal Media
  • DMEMF12
  • DMEM F12
  • Dulbecco's Modified Eagle Medium/Ham's F-12
  • Serum-Free Basal Media
Background InformationDMEM/F-12 PLUS is an advanced formulation of Dulbecco's Modified Eagle Medium/Ham's F-12 basal media that can be used in a broad array of cell lines for applications that require reduced or no serum supplementation. Cell lines cultured in DMEM/F12 PLUS and reduced serum exhibit equivalent or superior cell growth while maintaining the same cell morphology and function as in high serum conditions. Applications for DMEM/F12 PLUS include but are not limited to the following:

• Culture of primary, stem cells and cell lines in applications that require reduced or serum-free medium.
• Optimization of transfections that require reduced or serum-free conditions.
• Production of conditioned medium in reduced serum or serum-free conditions.
• Optimized media for the culture and expansion of multiple 3D organoids and tissues systems.

DMEM/F12 PLUS is a proprietary formulation that contains human serum albumin (HSA), defined lipids, trace elements, chemicals and proteins essential for optimal cell growth in reduced serum conditions. The media contains high-glucose and does not contain L-glutamine or penicillin-streptomycin.
Applications
ApplicationAdvanced DMEMF12 basal media used in low-serum or serum-free media formulations including stem cell and 3D organoid cell cultures.
Key Applications
  • Cell Culture
  • Stem Cell Culture
Biological Information
Media FormLiquid
Product Usage Statements
Quality AssuranceAppearance (color): Clear, Red Liquid
Osmolality: 290-330 mOsm
pH: 7.2-7.4
Sterility Tested: No Growth/Pass
Endotoxin: <2 EU/mL
Mycoplasma: Negative
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStore at 2-8°C. Before use, aliquot into smaller volumes to avoid contamination. Protect from light when not in use. Acclimate the medium at room temperature for 15-20 minutes before use. Do not warm media at 37°C before use.
Packaging Information
Material Size500 ml
Global Trade Item Number
Catalogue Number GTIN
SCM162 04054839755576