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S8001M CpGenome™ Human Methylated DNA Standard Set

It is intended for use as a positive control in gene methylation studies, such as bisulfite conversion of DNA with the CpGenome Turbo Bisulfite Modification Kit.

CpGenome™ Human Methylated DNA Standard Set MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
S8001M
5 µg   
Purchase on Sigma-Aldrich

Overview

Replacement Information
Description
Catalogue NumberS8001M
Brand Family Upstate
Trade Name
  • CpGenome
  • Upstate
DescriptionCpGenome™ Human Methylated DNA Standard Set
OverviewThe CpGenome™ Human Methylated DNA Standard is purified from HCT116 DKO cells that contain genetic knockouts of DNA methyltransferases, DNMT1 (-/-) and DNMT3b (-/-) , and have less than 5% methylated DNA. This Standard has been methylated enzymatically at all CpG dinucleotides by M. SssI methyltransferase. It is intended for use as a positive control in gene methylation studies, such as bisulfite conversion of DNA with the CpGenome™ Turbo Bisulfite Modification Kit (Cat. No. S7847). Bisulfite-modified DNA can be evaluated by methylation-specific PCR (MSP) technology using the CpG WIZ® amplification kits.

Materials Provided:

One vial containing 5 µg (20 µL) of CpGenome™ Human Methylated DNA Standard at a concentration of 250 ng/µL.

Validation:

Methylation-specific PCR (MSP) was performed on the CpGenome™ Human Methylated DNA after bisulfite modification with the CpGenome™ Turbo Bisulfite kit (Cat. No. S7847). Three sets of primers from the CpG WIZ® BRCA1 amplification kit (Cat. No. S7830) were used in this assay: the U primer set, which anneals to unmethylated bisulfite-modified DNA; M primers, which anneal to methylated bisulfite-modified sequences; and W primers, which anneal to unmethylated or methylated DNA that has not undergone bisulfite modification. Only the M primers generated product for the methylated DNA standard.

CpGenome and CpG WIZ are trademarks of Serologicals Corporation. CpG WIZ Methylation Products apply to technologies exclusively licensed from The Johns Hopkins University School of Medicine. Methylation-specific PCR (MSP) technology is covered under U.S. Patent # 5,786,146.
References
Product Information
PresentationLiquid in buffer containing 10 mM Tris-HCl, 1 mM EDTA, pH 8.0
Quality LevelMQ100
Applications
ApplicationIt is intended for use as a positive control in gene methylation studies, such as bisulfite conversion of DNA with the CpGenome Turbo Bisulfite Modification Kit.
Application NotesFor MSP primer design, please use the MethPrime software package. Click here
Biological Information
Species Reactivity
  • Human
Modifications
  • Methylation
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsRecommended Storage: Stable at -20°C for up to 6 months from date of receipt.
Packaging Information
Material Size5 µg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
S8001M 04053252648403

Documentation

CpGenome™ Human Methylated DNA Standard Set SDS

Title

Safety Data Sheet (SDS) 

CpGenome™ Human Methylated DNA Standard Set Certificates of Analysis

TitleLot Number
CpGenome Human Methylated DNA Standard - Q2023991 Q2023991
CpGenome™ Human Methylated 3069337
CpGenome™ Human Methylated -2615095 2615095
CpGenome™ Human Methylated -2785785 2785785
CpGenome™ Human Methylated -2808231 2808231
CpGenome™ Human Methylated DNA Standard 2918432
CpGenome™ Human Methylated DNA Standard 2928419
CpGenome™ Human Methylated DNA Standard 2964802
CpGenome™ Human Methylated DNA Standard 3147003
CpGenome™ Human Methylated DNA Standard 3131189
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Brochure

Title
Shaping Epigenetics Discovery - DNA Methylation Brochure

Technical Info

Title
White Paper - The Message in the Marks: Deciphering Cancer Epigenetics